Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5473 and Pf6N2E2_5474 are separated by 198 nucleotides Pf6N2E2_5474 and Pf6N2E2_5475 are separated by 160 nucleotides
Pf6N2E2_5473 - hydrolase, alpha/beta fold family, at 6,289,509 to 6,290,528
_5473
Pf6N2E2_5474 - Glycine cleavage system transcriptional activator, at 6,290,727 to 6,291,608
_5474
Pf6N2E2_5475 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 6,291,769 to 6,293,406
_5475
Position (kb)
6290
6291
6292 Strain fitness (log2 ratio)
-2
-1
0
1 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.931 kb on - strand, within Pf6N2E2_5473 at 6290.196 kb on + strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.224 kb on + strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.454 kb on + strand at 6290.455 kb on - strand at 6290.614 kb on + strand at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.641 kb on + strand at 6291.641 kb on + strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.642 kb on - strand at 6291.644 kb on + strand at 6291.645 kb on - strand at 6291.671 kb on - strand at 6291.745 kb on - strand at 6291.788 kb on + strand at 6291.788 kb on + strand at 6291.789 kb on - strand at 6291.800 kb on + strand at 6291.801 kb on - strand at 6291.801 kb on - strand at 6291.862 kb on + strand at 6291.863 kb on - strand at 6292.603 kb on + strand, within Pf6N2E2_5475
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 6,289,860 + Pf6N2E2_5473 0.34 -0.0 6,289,860 + Pf6N2E2_5473 0.34 -1.2 6,289,860 + Pf6N2E2_5473 0.34 +1.5 6,289,861 - Pf6N2E2_5473 0.35 +0.1 6,289,861 - Pf6N2E2_5473 0.35 +0.1 6,289,862 + Pf6N2E2_5473 0.35 +0.9 6,289,862 + Pf6N2E2_5473 0.35 +0.3 6,289,862 + Pf6N2E2_5473 0.35 -0.4 6,289,862 + Pf6N2E2_5473 0.35 +0.2 6,289,862 + Pf6N2E2_5473 0.35 +0.5 6,289,862 + Pf6N2E2_5473 0.35 +0.4 6,289,862 + Pf6N2E2_5473 0.35 +1.0 6,289,862 + Pf6N2E2_5473 0.35 -0.6 6,289,862 + Pf6N2E2_5473 0.35 -0.9 6,289,863 - Pf6N2E2_5473 0.35 -0.2 6,289,863 - Pf6N2E2_5473 0.35 +0.2 6,289,863 - Pf6N2E2_5473 0.35 -1.0 6,289,863 - Pf6N2E2_5473 0.35 +0.4 6,289,863 - Pf6N2E2_5473 0.35 +0.0 6,289,863 - Pf6N2E2_5473 0.35 -0.3 6,289,863 - Pf6N2E2_5473 0.35 +0.5 6,289,930 + Pf6N2E2_5473 0.41 -0.0 6,289,930 + Pf6N2E2_5473 0.41 +1.1 6,289,930 + Pf6N2E2_5473 0.41 +0.1 6,289,931 - Pf6N2E2_5473 0.41 +0.3 6,290,196 + Pf6N2E2_5473 0.67 +0.5 6,290,197 - Pf6N2E2_5473 0.67 +0.7 6,290,197 - Pf6N2E2_5473 0.67 +0.3 6,290,197 - Pf6N2E2_5473 0.67 -0.0 6,290,224 + Pf6N2E2_5473 0.70 -0.8 6,290,225 - Pf6N2E2_5473 0.70 -0.3 6,290,225 - Pf6N2E2_5473 0.70 +0.3 6,290,454 + -1.9 6,290,455 - +0.3 6,290,614 + +0.7 6,291,144 + Pf6N2E2_5474 0.47 -0.8 6,291,144 + Pf6N2E2_5474 0.47 -0.0 6,291,144 + Pf6N2E2_5474 0.47 -0.2 6,291,144 + Pf6N2E2_5474 0.47 +0.1 6,291,144 + Pf6N2E2_5474 0.47 +0.4 6,291,144 + Pf6N2E2_5474 0.47 -0.5 6,291,144 + Pf6N2E2_5474 0.47 -0.4 6,291,144 + Pf6N2E2_5474 0.47 +0.0 6,291,144 + Pf6N2E2_5474 0.47 +0.7 6,291,144 + Pf6N2E2_5474 0.47 +0.8 6,291,144 + Pf6N2E2_5474 0.47 -0.3 6,291,144 + Pf6N2E2_5474 0.47 +0.7 6,291,145 - Pf6N2E2_5474 0.47 -1.9 6,291,145 - Pf6N2E2_5474 0.47 +1.0 6,291,145 - Pf6N2E2_5474 0.47 -0.3 6,291,145 - Pf6N2E2_5474 0.47 +0.8 6,291,145 - Pf6N2E2_5474 0.47 -0.4 6,291,145 - Pf6N2E2_5474 0.47 -0.2 6,291,145 - Pf6N2E2_5474 0.47 +0.8 6,291,145 - Pf6N2E2_5474 0.47 -0.1 6,291,145 - Pf6N2E2_5474 0.47 +0.9 6,291,641 + +0.1 6,291,641 + +0.4 6,291,642 - -0.9 6,291,642 - -0.0 6,291,642 - -0.4 6,291,642 - +0.4 6,291,642 - -1.0 6,291,644 + +1.0 6,291,645 - +0.9 6,291,671 - -1.2 6,291,745 - -0.8 6,291,788 + +0.0 6,291,788 + -0.7 6,291,789 - -0.2 6,291,800 + -0.2 6,291,801 - -1.7 6,291,801 - -0.1 6,291,862 + +1.2 6,291,863 - +0.2 6,292,603 + Pf6N2E2_5475 0.51 -0.0
Or see this region's nucleotide sequence