Experiment: Gelatin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1530 and grxA are separated by 53 nucleotides grxA and Shew_1532 are separated by 65 nucleotides Shew_1532 and Shew_1533 are separated by 123 nucleotides
Shew_1530: Shew_1530 - porin (RefSeq), at 1,754,711 to 1,755,853
_1530
Shew_1531: grxA - glutaredoxin 1 (RefSeq), at 1,755,907 to 1,756,164
grxA
Shew_1532: Shew_1532 - type III restriction enzyme, res subunit (RefSeq), at 1,756,230 to 1,757,969
_1532
Shew_1533: Shew_1533 - acetyl-CoA synthetase (RefSeq), at 1,758,093 to 1,760,045
_1533
Position (kb)
1756
1757
1758 Strain fitness (log2 ratio)
-1
0
1 at 1755.354 kb on - strand, within Shew_1530 at 1755.730 kb on + strand, within Shew_1530 at 1755.756 kb on - strand at 1755.767 kb on + strand at 1756.001 kb on + strand, within grxA at 1756.124 kb on - strand, within grxA at 1756.206 kb on - strand at 1757.147 kb on + strand, within Shew_1532 at 1757.157 kb on + strand, within Shew_1532 at 1757.160 kb on - strand, within Shew_1532 at 1757.443 kb on + strand, within Shew_1532 at 1757.691 kb on + strand, within Shew_1532 at 1757.964 kb on - strand at 1758.232 kb on + strand at 1758.422 kb on + strand, within Shew_1533 at 1758.632 kb on - strand, within Shew_1533
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (C) remove 1,755,354 - Shew_1530 0.56 -0.1 1,755,730 + Shew_1530 0.89 -0.1 1,755,756 - -0.1 1,755,767 + -0.5 1,756,001 + grxA Shew_1531 0.36 +1.2 1,756,124 - grxA Shew_1531 0.84 +0.3 1,756,206 - -0.1 1,757,147 + Shew_1532 0.53 +0.8 1,757,157 + Shew_1532 0.53 -0.4 1,757,160 - Shew_1532 0.53 +0.1 1,757,443 + Shew_1532 0.70 -0.3 1,757,691 + Shew_1532 0.84 +0.1 1,757,964 - +0.3 1,758,232 + +0.4 1,758,422 + Shew_1533 0.17 +0.2 1,758,632 - Shew_1533 0.28 +0.6
Or see this region's nucleotide sequence