Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2363

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2362 and Pf6N2E2_2363 overlap by 26 nucleotidesPf6N2E2_2363 and Pf6N2E2_2364 are separated by 4 nucleotidesPf6N2E2_2364 and Pf6N2E2_2365 are separated by 8 nucleotidesPf6N2E2_2365 and Pf6N2E2_2366 overlap by 8 nucleotides Pf6N2E2_2362 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 2,797,341 to 2,798,375 _2362 Pf6N2E2_2363 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 2,798,350 to 2,799,153 _2363 Pf6N2E2_2364 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 2,799,158 to 2,799,412 _2364 Pf6N2E2_2365 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 2,799,421 to 2,800,080 _2365 Pf6N2E2_2366 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 2,800,073 to 2,800,453 _2366 Position (kb) 2798 2799 2800Strain fitness (log2 ratio) -1 0 1at 2797.417 kb on + strandat 2797.417 kb on + strandat 2797.492 kb on + strand, within Pf6N2E2_2362at 2797.492 kb on + strand, within Pf6N2E2_2362at 2797.492 kb on + strand, within Pf6N2E2_2362at 2797.570 kb on + strand, within Pf6N2E2_2362at 2797.570 kb on + strand, within Pf6N2E2_2362at 2797.766 kb on - strand, within Pf6N2E2_2362at 2797.948 kb on + strand, within Pf6N2E2_2362at 2797.949 kb on - strand, within Pf6N2E2_2362at 2798.207 kb on - strand, within Pf6N2E2_2362at 2798.248 kb on + strand, within Pf6N2E2_2362at 2798.249 kb on - strand, within Pf6N2E2_2362at 2798.465 kb on + strand, within Pf6N2E2_2363at 2798.659 kb on + strand, within Pf6N2E2_2363at 2798.693 kb on + strand, within Pf6N2E2_2363at 2798.745 kb on - strand, within Pf6N2E2_2363at 2798.908 kb on + strand, within Pf6N2E2_2363at 2798.985 kb on - strand, within Pf6N2E2_2363at 2799.125 kb on + strandat 2799.126 kb on - strandat 2799.159 kb on + strandat 2799.440 kb on + strandat 2799.599 kb on + strand, within Pf6N2E2_2365at 2799.599 kb on + strand, within Pf6N2E2_2365at 2799.599 kb on + strand, within Pf6N2E2_2365at 2799.605 kb on + strand, within Pf6N2E2_2365at 2799.605 kb on + strand, within Pf6N2E2_2365at 2799.605 kb on + strand, within Pf6N2E2_2365at 2799.606 kb on - strand, within Pf6N2E2_2365at 2799.768 kb on - strand, within Pf6N2E2_2365at 2799.779 kb on + strand, within Pf6N2E2_2365at 2799.788 kb on + strand, within Pf6N2E2_2365at 2799.789 kb on - strand, within Pf6N2E2_2365at 2799.789 kb on - strand, within Pf6N2E2_2365at 2799.838 kb on - strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.860 kb on + strand, within Pf6N2E2_2365at 2799.861 kb on - strand, within Pf6N2E2_2365at 2799.861 kb on - strand, within Pf6N2E2_2365at 2799.861 kb on - strand, within Pf6N2E2_2365at 2799.864 kb on - strand, within Pf6N2E2_2365at 2799.864 kb on - strand, within Pf6N2E2_2365at 2800.004 kb on + strand, within Pf6N2E2_2365at 2800.005 kb on - strand, within Pf6N2E2_2365

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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2,797,417 + +0.4
2,797,417 + +1.1
2,797,492 + Pf6N2E2_2362 0.15 +0.0
2,797,492 + Pf6N2E2_2362 0.15 -0.2
2,797,492 + Pf6N2E2_2362 0.15 -0.2
2,797,570 + Pf6N2E2_2362 0.22 -0.5
2,797,570 + Pf6N2E2_2362 0.22 +0.4
2,797,766 - Pf6N2E2_2362 0.41 +0.1
2,797,948 + Pf6N2E2_2362 0.59 -0.4
2,797,949 - Pf6N2E2_2362 0.59 +0.2
2,798,207 - Pf6N2E2_2362 0.84 +1.7
2,798,248 + Pf6N2E2_2362 0.88 +0.3
2,798,249 - Pf6N2E2_2362 0.88 -0.8
2,798,465 + Pf6N2E2_2363 0.14 -0.7
2,798,659 + Pf6N2E2_2363 0.38 +0.2
2,798,693 + Pf6N2E2_2363 0.43 -0.8
2,798,745 - Pf6N2E2_2363 0.49 -0.7
2,798,908 + Pf6N2E2_2363 0.69 -0.5
2,798,985 - Pf6N2E2_2363 0.79 -0.1
2,799,125 + +0.7
2,799,126 - +0.4
2,799,159 + +0.6
2,799,440 + -0.2
2,799,599 + Pf6N2E2_2365 0.27 +0.7
2,799,599 + Pf6N2E2_2365 0.27 +1.0
2,799,599 + Pf6N2E2_2365 0.27 -0.6
2,799,605 + Pf6N2E2_2365 0.28 -0.0
2,799,605 + Pf6N2E2_2365 0.28 +0.6
2,799,605 + Pf6N2E2_2365 0.28 +0.1
2,799,606 - Pf6N2E2_2365 0.28 -0.4
2,799,768 - Pf6N2E2_2365 0.53 +0.6
2,799,779 + Pf6N2E2_2365 0.54 +0.6
2,799,788 + Pf6N2E2_2365 0.56 -0.1
2,799,789 - Pf6N2E2_2365 0.56 +0.3
2,799,789 - Pf6N2E2_2365 0.56 +0.4
2,799,838 - Pf6N2E2_2365 0.63 +0.7
2,799,860 + Pf6N2E2_2365 0.67 +0.8
2,799,860 + Pf6N2E2_2365 0.67 -0.1
2,799,860 + Pf6N2E2_2365 0.67 +0.0
2,799,860 + Pf6N2E2_2365 0.67 -0.1
2,799,860 + Pf6N2E2_2365 0.67 +0.4
2,799,861 - Pf6N2E2_2365 0.67 -1.0
2,799,861 - Pf6N2E2_2365 0.67 +0.6
2,799,861 - Pf6N2E2_2365 0.67 +0.1
2,799,864 - Pf6N2E2_2365 0.67 +1.4
2,799,864 - Pf6N2E2_2365 0.67 -0.5
2,800,004 + Pf6N2E2_2365 0.88 +0.5
2,800,005 - Pf6N2E2_2365 0.88 +0.4

Or see this region's nucleotide sequence