Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folD and tRNA-Pro-1 are separated by 354 nucleotides tRNA-Pro-1 and tRNA-Arg-1 are separated by 90 nucleotides tRNA-Arg-1 and tRNA-His-1 are separated by 9 nucleotides tRNA-His-1 and tig are separated by 261 nucleotides tig and clpP are separated by 89 nucleotides clpP and clpX are separated by 99 nucleotides
SO1792: folD - methylenetetrahydrofolate dehydrogenase/methylenetetrahydrofolate cyclohydrolase (NCBI ptt file), at 1,878,808 to 1,879,662
folD
SO_t024: tRNA-Pro-1 - tRNA-Pro (RefSeq), at 1,880,017 to 1,880,093
tRNA-Pro-1
SO_t025: tRNA-Arg-1 - tRNA-Arg (RefSeq), at 1,880,184 to 1,880,260
tRNA-Arg-1
SO_t026: tRNA-His-1 - tRNA-His (RefSeq), at 1,880,270 to 1,880,345
tRNA-His-1
SO1793: tig - trigger factor (NCBI ptt file), at 1,880,607 to 1,881,911
tig
SO1794: clpP - ATP-dependent Clp protease, proteolytic subunit (NCBI ptt file), at 1,882,001 to 1,882,609
clpP
SO1795: clpX - ATP-dependent Clp protease, ATP-binding subunit ClpX (NCBI ptt file), at 1,882,709 to 1,883,989
clpX
Position (kb)
1880
1881
1882 Strain fitness (log2 ratio)
-2
-1
0
1 at 1879.665 kb on - strand at 1880.454 kb on - strand at 1880.783 kb on - strand, within tig at 1881.009 kb on + strand, within tig at 1881.017 kb on - strand, within tig at 1881.017 kb on - strand, within tig at 1881.062 kb on + strand, within tig at 1881.117 kb on + strand, within tig at 1881.371 kb on - strand, within tig at 1881.597 kb on - strand, within tig at 1881.836 kb on + strand at 1881.836 kb on + strand at 1881.900 kb on - strand at 1882.028 kb on + strand at 1882.278 kb on + strand, within clpP at 1882.285 kb on + strand, within clpP at 1882.423 kb on - strand, within clpP at 1882.594 kb on + strand at 1882.625 kb on + strand at 1882.633 kb on - strand at 1882.666 kb on + strand at 1882.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 1,879,665 - -0.3 1,880,454 - +0.5 1,880,783 - tig SO1793 0.13 -1.5 1,881,009 + tig SO1793 0.31 -0.6 1,881,017 - tig SO1793 0.31 -1.0 1,881,017 - tig SO1793 0.31 -2.1 1,881,062 + tig SO1793 0.35 +0.8 1,881,117 + tig SO1793 0.39 -0.9 1,881,371 - tig SO1793 0.59 -0.4 1,881,597 - tig SO1793 0.76 -0.9 1,881,836 + +1.2 1,881,836 + +0.0 1,881,900 - -1.4 1,882,028 + -1.0 1,882,278 + clpP SO1794 0.45 -1.9 1,882,285 + clpP SO1794 0.47 -1.2 1,882,423 - clpP SO1794 0.69 -0.6 1,882,594 + -0.2 1,882,625 + +0.0 1,882,633 - -1.3 1,882,666 + -0.6 1,882,819 - -1.4
Or see this region's nucleotide sequence