Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheB-3 and SO3207 are separated by 20 nucleotides SO3207 and cheZ are separated by 15 nucleotides cheZ and cheY-3 are separated by 13 nucleotides
SO3206: cheB-3 - protein-glutamate methylesterase CheB (NCBI ptt file), at 3,345,536 to 3,346,660
cheB-3
SO3207: SO3207 - chemotaxis protein (NCBI ptt file), at 3,346,681 to 3,348,957
SO3207
SO3208: cheZ - chemotaxis protein CheZ (NCBI ptt file), at 3,348,973 to 3,349,710
cheZ
SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107
cheY-3
Position (kb)
3346
3347
3348
3349 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3345.764 kb on - strand, within cheB-3 at 3345.800 kb on - strand, within cheB-3 at 3345.840 kb on - strand at 3345.996 kb on + strand, within cheB-3 at 3346.075 kb on - strand, within cheB-3 at 3346.101 kb on + strand, within cheB-3 at 3346.209 kb on - strand, within cheB-3 at 3346.369 kb on + strand, within cheB-3 at 3346.378 kb on + strand, within cheB-3 at 3346.421 kb on + strand, within cheB-3 at 3346.434 kb on - strand, within cheB-3 at 3346.475 kb on + strand, within cheB-3 at 3346.527 kb on + strand, within cheB-3 at 3346.535 kb on - strand, within cheB-3 at 3346.730 kb on - strand at 3346.858 kb on - strand at 3346.861 kb on + strand at 3346.873 kb on - strand at 3346.944 kb on + strand, within SO3207 at 3346.993 kb on - strand, within SO3207 at 3347.014 kb on - strand, within SO3207 at 3347.144 kb on + strand, within SO3207 at 3347.152 kb on - strand, within SO3207 at 3347.152 kb on - strand, within SO3207 at 3347.172 kb on - strand, within SO3207 at 3347.172 kb on - strand, within SO3207 at 3347.185 kb on - strand, within SO3207 at 3347.447 kb on + strand, within SO3207 at 3347.447 kb on + strand, within SO3207 at 3347.455 kb on - strand, within SO3207 at 3347.455 kb on - strand, within SO3207 at 3347.504 kb on + strand, within SO3207 at 3347.507 kb on - strand, within SO3207 at 3348.181 kb on - strand, within SO3207 at 3348.325 kb on - strand, within SO3207 at 3348.366 kb on - strand, within SO3207 at 3348.466 kb on + strand, within SO3207 at 3348.575 kb on - strand, within SO3207 at 3348.578 kb on - strand, within SO3207 at 3348.707 kb on + strand, within SO3207 at 3348.712 kb on + strand, within SO3207 at 3348.712 kb on + strand, within SO3207 at 3348.720 kb on - strand, within SO3207 at 3348.720 kb on - strand, within SO3207 at 3348.720 kb on - strand, within SO3207 at 3348.762 kb on + strand at 3348.782 kb on - strand at 3348.799 kb on + strand at 3348.799 kb on + strand at 3348.840 kb on + strand at 3348.922 kb on + strand at 3349.009 kb on + strand at 3349.017 kb on - strand at 3349.017 kb on - strand at 3349.017 kb on - strand at 3349.017 kb on - strand at 3349.113 kb on + strand, within cheZ at 3349.302 kb on + strand, within cheZ at 3349.656 kb on - strand at 3349.666 kb on - strand at 3349.689 kb on + strand at 3349.712 kb on + strand at 3349.713 kb on - strand at 3349.720 kb on - strand at 3349.767 kb on - strand, within cheY-3 at 3349.789 kb on + strand, within cheY-3 at 3349.899 kb on + strand, within cheY-3
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 3,345,764 - cheB-3 SO3206 0.20 +1.0 3,345,800 - cheB-3 SO3206 0.23 +0.7 3,345,840 - +2.8 3,345,996 + cheB-3 SO3206 0.41 -0.1 3,346,075 - cheB-3 SO3206 0.48 -0.3 3,346,101 + cheB-3 SO3206 0.50 +0.2 3,346,209 - cheB-3 SO3206 0.60 +0.5 3,346,369 + cheB-3 SO3206 0.74 -0.2 3,346,378 + cheB-3 SO3206 0.75 -0.3 3,346,421 + cheB-3 SO3206 0.79 -1.2 3,346,434 - cheB-3 SO3206 0.80 -1.4 3,346,475 + cheB-3 SO3206 0.83 +0.4 3,346,527 + cheB-3 SO3206 0.88 -1.2 3,346,535 - cheB-3 SO3206 0.89 -0.6 3,346,730 - -2.3 3,346,858 - +0.2 3,346,861 + -1.7 3,346,873 - -1.4 3,346,944 + SO3207 0.12 -0.6 3,346,993 - SO3207 0.14 -1.4 3,347,014 - SO3207 0.15 -1.8 3,347,144 + SO3207 0.20 -2.2 3,347,152 - SO3207 0.21 -2.8 3,347,152 - SO3207 0.21 -0.5 3,347,172 - SO3207 0.22 -2.2 3,347,172 - SO3207 0.22 -1.6 3,347,185 - SO3207 0.22 -1.1 3,347,447 + SO3207 0.34 -1.3 3,347,447 + SO3207 0.34 +0.9 3,347,455 - SO3207 0.34 -1.3 3,347,455 - SO3207 0.34 -0.7 3,347,504 + SO3207 0.36 -1.1 3,347,507 - SO3207 0.36 -2.1 3,348,181 - SO3207 0.66 -0.4 3,348,325 - SO3207 0.72 -0.4 3,348,366 - SO3207 0.74 -0.6 3,348,466 + SO3207 0.78 -1.1 3,348,575 - SO3207 0.83 -1.2 3,348,578 - SO3207 0.83 -2.0 3,348,707 + SO3207 0.89 -1.7 3,348,712 + SO3207 0.89 -1.4 3,348,712 + SO3207 0.89 -0.9 3,348,720 - SO3207 0.90 -0.9 3,348,720 - SO3207 0.90 -1.5 3,348,720 - SO3207 0.90 -1.4 3,348,762 + -2.3 3,348,782 - -0.8 3,348,799 + -0.9 3,348,799 + -0.7 3,348,840 + -1.9 3,348,922 + -1.5 3,349,009 + -2.4 3,349,017 - -0.8 3,349,017 - +0.7 3,349,017 - +0.1 3,349,017 - -0.1 3,349,113 + cheZ SO3208 0.19 -1.4 3,349,302 + cheZ SO3208 0.45 -1.1 3,349,656 - -0.2 3,349,666 - -0.2 3,349,689 + -2.8 3,349,712 + -3.5 3,349,713 - +0.3 3,349,720 - -1.2 3,349,767 - cheY-3 SO3209 0.11 -2.4 3,349,789 + cheY-3 SO3209 0.17 -2.5 3,349,899 + cheY-3 SO3209 0.46 -2.6
Or see this region's nucleotide sequence