Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20725

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20715 and BPHYT_RS20720 are separated by 419 nucleotidesBPHYT_RS20720 and BPHYT_RS20725 are separated by 18 nucleotidesBPHYT_RS20725 and BPHYT_RS20730 are separated by 108 nucleotides BPHYT_RS20715: BPHYT_RS20715 - peptidase, at 208,196 to 209,902 _RS20715 BPHYT_RS20720: BPHYT_RS20720 - hypothetical protein, at 210,322 to 210,588 _RS20720 BPHYT_RS20725: BPHYT_RS20725 - histidine kinase, at 210,607 to 211,944 _RS20725 BPHYT_RS20730: BPHYT_RS20730 - TetR family transcriptional regulator, at 212,053 to 212,787 _RS20730 Position (kb) 210 211 212Strain fitness (log2 ratio) -2 -1 0 1at 210.439 kb on + strand, within BPHYT_RS20720at 210.440 kb on - strand, within BPHYT_RS20720at 210.574 kb on + strandat 211.464 kb on + strand, within BPHYT_RS20725at 211.958 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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210,439 + BPHYT_RS20720 0.44 +0.4
210,440 - BPHYT_RS20720 0.44 -1.1
210,574 + +1.4
211,464 + BPHYT_RS20725 0.64 -2.0
211,958 + +0.6

Or see this region's nucleotide sequence