Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17665 and BPHYT_RS17670 are separated by 94 nucleotides BPHYT_RS17670 and BPHYT_RS17675 overlap by 4 nucleotides BPHYT_RS17675 and BPHYT_RS17680 are separated by 4 nucleotides BPHYT_RS17680 and BPHYT_RS17685 are separated by 237 nucleotides
BPHYT_RS17665: BPHYT_RS17665 - membrane protein, at 3,994,175 to 3,994,570
_RS17665
BPHYT_RS17670: BPHYT_RS17670 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 3,994,665 to 3,995,060
_RS17670
BPHYT_RS17675: BPHYT_RS17675 - phosphoribosyl-AMP cyclohydrolase, at 3,995,057 to 3,995,464
_RS17675
BPHYT_RS17680: BPHYT_RS17680 - imidazole glycerol phosphate synthase subunit HisF, at 3,995,469 to 3,996,242
_RS17680
BPHYT_RS17685: BPHYT_RS17685 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 3,996,480 to 3,997,232
_RS17685
Position (kb)
3995
3996
3997 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3994.473 kb on + strand, within BPHYT_RS17665 at 3994.492 kb on + strand, within BPHYT_RS17665 at 3994.549 kb on - strand at 3994.660 kb on + strand at 3994.948 kb on + strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3994.949 kb on - strand, within BPHYT_RS17670 at 3995.009 kb on + strand, within BPHYT_RS17670 at 3995.009 kb on + strand, within BPHYT_RS17670 at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.064 kb on + strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.065 kb on - strand at 3995.305 kb on - strand, within BPHYT_RS17675 at 3995.536 kb on + strand at 3995.666 kb on + strand, within BPHYT_RS17680 at 3995.881 kb on + strand, within BPHYT_RS17680 at 3995.882 kb on - strand, within BPHYT_RS17680 at 3995.882 kb on - strand, within BPHYT_RS17680 at 3996.230 kb on + strand at 3996.230 kb on + strand at 3996.235 kb on - strand at 3996.235 kb on - strand at 3996.482 kb on - strand at 3996.482 kb on - strand at 3996.733 kb on + strand, within BPHYT_RS17685 at 3996.754 kb on + strand, within BPHYT_RS17685 at 3996.755 kb on - strand, within BPHYT_RS17685 at 3996.857 kb on + strand, within BPHYT_RS17685 at 3996.857 kb on + strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.932 kb on - strand, within BPHYT_RS17685 at 3996.952 kb on + strand, within BPHYT_RS17685 at 3996.953 kb on - strand, within BPHYT_RS17685 at 3997.188 kb on + strand at 3997.188 kb on + strand at 3997.188 kb on + strand at 3997.189 kb on - strand at 3997.232 kb on + strand at 3997.232 kb on + strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand at 3997.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 3,994,473 + BPHYT_RS17665 0.75 +0.8 3,994,492 + BPHYT_RS17665 0.80 -0.6 3,994,549 - +0.3 3,994,660 + +0.2 3,994,948 + BPHYT_RS17670 0.71 -2.9 3,994,949 - BPHYT_RS17670 0.72 +0.4 3,994,949 - BPHYT_RS17670 0.72 +0.7 3,994,949 - BPHYT_RS17670 0.72 -0.6 3,995,009 + BPHYT_RS17670 0.87 -1.6 3,995,009 + BPHYT_RS17670 0.87 -2.2 3,995,064 + +0.0 3,995,064 + -0.2 3,995,064 + +0.5 3,995,064 + +0.9 3,995,064 + +1.3 3,995,065 - +0.8 3,995,065 - -0.2 3,995,065 - +0.1 3,995,065 - -0.0 3,995,305 - BPHYT_RS17675 0.61 +0.9 3,995,536 + -1.3 3,995,666 + BPHYT_RS17680 0.25 +0.3 3,995,881 + BPHYT_RS17680 0.53 -1.0 3,995,882 - BPHYT_RS17680 0.53 -0.7 3,995,882 - BPHYT_RS17680 0.53 +0.6 3,996,230 + +1.1 3,996,230 + -0.5 3,996,235 - -0.2 3,996,235 - -1.2 3,996,482 - +1.3 3,996,482 - +0.4 3,996,733 + BPHYT_RS17685 0.34 +1.5 3,996,754 + BPHYT_RS17685 0.36 -0.6 3,996,755 - BPHYT_RS17685 0.37 +1.0 3,996,857 + BPHYT_RS17685 0.50 -2.8 3,996,857 + BPHYT_RS17685 0.50 +1.3 3,996,932 - BPHYT_RS17685 0.60 -0.7 3,996,932 - BPHYT_RS17685 0.60 -0.1 3,996,932 - BPHYT_RS17685 0.60 +0.2 3,996,952 + BPHYT_RS17685 0.63 -1.2 3,996,953 - BPHYT_RS17685 0.63 -1.5 3,997,188 + -0.2 3,997,188 + -0.3 3,997,188 + +3.1 3,997,189 - -0.4 3,997,232 + -2.1 3,997,232 + -0.8 3,997,233 - -0.0 3,997,233 - -0.4 3,997,233 - +0.4 3,997,233 - +0.4 3,997,233 - -0.9
Or see this region's nucleotide sequence