Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_19

Experiment: Ying_Nucleobase14 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_18 and Pf6N2E2_19 are separated by 10 nucleotidesPf6N2E2_19 and Pf6N2E2_20 are separated by 0 nucleotides Pf6N2E2_18 - Outer membrane pyoverdine eflux protein, at 51,075 to 52,469 _18 Pf6N2E2_19 - Pyoverdine efflux carrier and ATP binding protein, at 52,480 to 54,450 _19 Pf6N2E2_20 - pyoverdine-specific efflux macA-like protein, at 54,451 to 55,602 _20 Position (kb) 52 53 54 55Strain fitness (log2 ratio) -2 -1 0 1at 51.734 kb on + strand, within Pf6N2E2_18at 51.735 kb on - strand, within Pf6N2E2_18at 51.937 kb on + strand, within Pf6N2E2_18at 51.937 kb on + strand, within Pf6N2E2_18at 51.938 kb on - strand, within Pf6N2E2_18at 51.938 kb on - strand, within Pf6N2E2_18at 51.938 kb on - strand, within Pf6N2E2_18at 52.060 kb on + strand, within Pf6N2E2_18at 52.060 kb on + strand, within Pf6N2E2_18at 52.061 kb on - strand, within Pf6N2E2_18at 52.084 kb on + strand, within Pf6N2E2_18at 52.084 kb on + strand, within Pf6N2E2_18at 52.085 kb on - strand, within Pf6N2E2_18at 52.087 kb on + strand, within Pf6N2E2_18at 52.088 kb on - strand, within Pf6N2E2_18at 52.130 kb on - strand, within Pf6N2E2_18at 53.012 kb on + strand, within Pf6N2E2_19at 53.012 kb on + strand, within Pf6N2E2_19at 53.058 kb on - strand, within Pf6N2E2_19at 53.160 kb on - strand, within Pf6N2E2_19at 53.261 kb on + strand, within Pf6N2E2_19at 53.261 kb on + strand, within Pf6N2E2_19at 53.262 kb on - strand, within Pf6N2E2_19at 53.324 kb on + strand, within Pf6N2E2_19at 53.325 kb on - strand, within Pf6N2E2_19at 53.325 kb on - strand, within Pf6N2E2_19at 53.325 kb on - strand, within Pf6N2E2_19at 53.481 kb on - strand, within Pf6N2E2_19at 54.107 kb on + strand, within Pf6N2E2_19at 54.107 kb on + strand, within Pf6N2E2_19at 54.161 kb on + strand, within Pf6N2E2_19at 54.712 kb on + strand, within Pf6N2E2_20at 54.712 kb on + strand, within Pf6N2E2_20at 54.731 kb on + strand, within Pf6N2E2_20at 54.732 kb on - strand, within Pf6N2E2_20at 54.732 kb on - strand, within Pf6N2E2_20at 54.732 kb on - strand, within Pf6N2E2_20at 54.732 kb on - strand, within Pf6N2E2_20at 54.872 kb on + strand, within Pf6N2E2_20at 54.873 kb on - strand, within Pf6N2E2_20at 55.422 kb on - strand, within Pf6N2E2_20

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3
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51,734 + Pf6N2E2_18 0.47 -0.6
51,735 - Pf6N2E2_18 0.47 +0.4
51,937 + Pf6N2E2_18 0.62 -0.6
51,937 + Pf6N2E2_18 0.62 +0.4
51,938 - Pf6N2E2_18 0.62 +0.4
51,938 - Pf6N2E2_18 0.62 +0.4
51,938 - Pf6N2E2_18 0.62 +0.4
52,060 + Pf6N2E2_18 0.71 +0.4
52,060 + Pf6N2E2_18 0.71 -0.6
52,061 - Pf6N2E2_18 0.71 +0.4
52,084 + Pf6N2E2_18 0.72 -0.5
52,084 + Pf6N2E2_18 0.72 -0.6
52,085 - Pf6N2E2_18 0.72 -1.4
52,087 + Pf6N2E2_18 0.73 -0.4
52,088 - Pf6N2E2_18 0.73 -0.1
52,130 - Pf6N2E2_18 0.76 +0.4
53,012 + Pf6N2E2_19 0.27 +0.4
53,012 + Pf6N2E2_19 0.27 -2.0
53,058 - Pf6N2E2_19 0.29 +0.4
53,160 - Pf6N2E2_19 0.35 -1.1
53,261 + Pf6N2E2_19 0.40 +0.4
53,261 + Pf6N2E2_19 0.40 -2.2
53,262 - Pf6N2E2_19 0.40 -0.6
53,324 + Pf6N2E2_19 0.43 -1.2
53,325 - Pf6N2E2_19 0.43 +0.4
53,325 - Pf6N2E2_19 0.43 -0.6
53,325 - Pf6N2E2_19 0.43 +0.4
53,481 - Pf6N2E2_19 0.51 -0.3
54,107 + Pf6N2E2_19 0.83 -1.6
54,107 + Pf6N2E2_19 0.83 -2.4
54,161 + Pf6N2E2_19 0.85 +0.4
54,712 + Pf6N2E2_20 0.23 -0.6
54,712 + Pf6N2E2_20 0.23 -0.6
54,731 + Pf6N2E2_20 0.24 -1.0
54,732 - Pf6N2E2_20 0.24 +0.4
54,732 - Pf6N2E2_20 0.24 -1.2
54,732 - Pf6N2E2_20 0.24 -0.8
54,732 - Pf6N2E2_20 0.24 +0.4
54,872 + Pf6N2E2_20 0.37 -0.8
54,873 - Pf6N2E2_20 0.37 +0.4
55,422 - Pf6N2E2_20 0.84 +0.0

Or see this region's nucleotide sequence