Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4978 and Pf6N2E2_4979 are separated by 274 nucleotides Pf6N2E2_4979 and Pf6N2E2_4980 are separated by 68 nucleotides Pf6N2E2_4980 and Pf6N2E2_4981 overlap by 27 nucleotides
Pf6N2E2_4978 - Ethanolamine permease, at 5,743,333 to 5,744,703
_4978
Pf6N2E2_4979 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,744,978 to 5,746,498
_4979
Pf6N2E2_4980 - hypothetical protein, at 5,746,567 to 5,747,094
_4980
Pf6N2E2_4981 - Nitrogen regulation protein NR(I), at 5,747,068 to 5,748,975
_4981
Position (kb) 5744
5745
5746
5747 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5743.993 kb on + strand, within Pf6N2E2_4978 at 5743.993 kb on + strand, within Pf6N2E2_4978 at 5743.994 kb on - strand, within Pf6N2E2_4978 at 5743.994 kb on - strand, within Pf6N2E2_4978 at 5744.015 kb on + strand, within Pf6N2E2_4978 at 5744.016 kb on - strand, within Pf6N2E2_4978 at 5744.181 kb on + strand, within Pf6N2E2_4978 at 5744.312 kb on + strand, within Pf6N2E2_4978 at 5744.313 kb on - strand, within Pf6N2E2_4978 at 5744.313 kb on - strand, within Pf6N2E2_4978 at 5744.367 kb on - strand, within Pf6N2E2_4978 at 5744.471 kb on + strand, within Pf6N2E2_4978 at 5744.472 kb on - strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.603 kb on + strand at 5744.604 kb on - strand at 5744.694 kb on + strand at 5744.694 kb on + strand at 5744.695 kb on - strand at 5744.695 kb on - strand at 5744.703 kb on + strand at 5744.703 kb on + strand at 5744.703 kb on + strand at 5744.703 kb on + strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.724 kb on - strand at 5744.724 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5745.006 kb on + strand at 5745.006 kb on + strand at 5745.036 kb on + strand at 5745.036 kb on + strand at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.351 kb on + strand, within Pf6N2E2_4979 at 5745.559 kb on - strand, within Pf6N2E2_4979 at 5745.559 kb on - strand, within Pf6N2E2_4979 at 5745.559 kb on - strand, within Pf6N2E2_4979 at 5746.053 kb on + strand, within Pf6N2E2_4979 at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.438 kb on + strand at 5746.443 kb on + strand at 5746.443 kb on + strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.465 kb on + strand at 5746.525 kb on + strand at 5746.525 kb on + strand at 5746.525 kb on + strand at 5746.526 kb on - strand at 5746.526 kb on - strand at 5746.527 kb on + strand at 5746.527 kb on + strand at 5746.527 kb on + strand at 5746.528 kb on - strand at 5746.528 kb on - strand at 5746.528 kb on - strand at 5746.562 kb on + strand at 5746.562 kb on + strand at 5746.562 kb on + strand at 5746.563 kb on - strand at 5746.563 kb on - strand at 5746.563 kb on - strand at 5746.568 kb on + strand at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.785 kb on - strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.787 kb on - strand, within Pf6N2E2_4980 at 5746.956 kb on + strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5747.059 kb on + strand at 5747.059 kb on + strand at 5747.062 kb on + strand at 5747.062 kb on + strand at 5747.063 kb on - strand at 5747.063 kb on - strand at 5747.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 5,743,993 + Pf6N2E2_4978 0.48 +0.4 5,743,993 + Pf6N2E2_4978 0.48 +0.4 5,743,994 - Pf6N2E2_4978 0.48 -1.8 5,743,994 - Pf6N2E2_4978 0.48 +0.4 5,744,015 + Pf6N2E2_4978 0.50 -1.2 5,744,016 - Pf6N2E2_4978 0.50 +0.4 5,744,181 + Pf6N2E2_4978 0.62 +0.4 5,744,312 + Pf6N2E2_4978 0.71 -0.6 5,744,313 - Pf6N2E2_4978 0.71 -0.6 5,744,313 - Pf6N2E2_4978 0.71 -1.2 5,744,367 - Pf6N2E2_4978 0.75 -0.4 5,744,471 + Pf6N2E2_4978 0.83 +0.4 5,744,472 - Pf6N2E2_4978 0.83 +0.4 5,744,540 + Pf6N2E2_4978 0.88 -1.2 5,744,540 + Pf6N2E2_4978 0.88 -1.2 5,744,540 + Pf6N2E2_4978 0.88 -1.4 5,744,540 + Pf6N2E2_4978 0.88 -0.6 5,744,540 + Pf6N2E2_4978 0.88 -0.8 5,744,540 + Pf6N2E2_4978 0.88 -0.6 5,744,540 + Pf6N2E2_4978 0.88 +0.4 5,744,541 - Pf6N2E2_4978 0.88 -1.2 5,744,541 - Pf6N2E2_4978 0.88 +0.4 5,744,541 - Pf6N2E2_4978 0.88 -0.4 5,744,541 - Pf6N2E2_4978 0.88 +0.4 5,744,541 - Pf6N2E2_4978 0.88 +0.4 5,744,541 - Pf6N2E2_4978 0.88 +0.4 5,744,603 + +0.4 5,744,604 - -0.2 5,744,694 + +0.4 5,744,694 + -0.6 5,744,695 - +0.4 5,744,695 - -1.1 5,744,703 + -0.8 5,744,703 + +0.4 5,744,703 + +0.4 5,744,703 + -1.1 5,744,704 - +0.4 5,744,704 - +0.4 5,744,704 - -0.6 5,744,704 - +0.4 5,744,704 - +0.4 5,744,724 - -1.2 5,744,724 - -0.8 5,744,980 - -1.2 5,744,980 - +0.4 5,744,980 - -1.4 5,744,980 - -1.2 5,744,980 - -0.8 5,745,006 + +0.4 5,745,006 + -1.2 5,745,036 + -1.0 5,745,036 + +0.4 5,745,348 + Pf6N2E2_4979 0.24 -1.1 5,745,348 + Pf6N2E2_4979 0.24 -1.2 5,745,348 + Pf6N2E2_4979 0.24 +0.4 5,745,348 + Pf6N2E2_4979 0.24 +0.4 5,745,348 + Pf6N2E2_4979 0.24 -0.6 5,745,348 + Pf6N2E2_4979 0.24 +0.4 5,745,348 + Pf6N2E2_4979 0.24 +0.4 5,745,348 + Pf6N2E2_4979 0.24 +0.4 5,745,348 + Pf6N2E2_4979 0.24 -1.2 5,745,348 + Pf6N2E2_4979 0.24 -1.2 5,745,348 + Pf6N2E2_4979 0.24 -0.6 5,745,349 - Pf6N2E2_4979 0.24 -0.6 5,745,349 - Pf6N2E2_4979 0.24 -1.6 5,745,349 - Pf6N2E2_4979 0.24 +1.0 5,745,349 - Pf6N2E2_4979 0.24 -0.9 5,745,349 - Pf6N2E2_4979 0.24 +0.4 5,745,349 - Pf6N2E2_4979 0.24 -2.6 5,745,349 - Pf6N2E2_4979 0.24 -1.6 5,745,349 - Pf6N2E2_4979 0.24 +0.4 5,745,349 - Pf6N2E2_4979 0.24 +0.4 5,745,349 - Pf6N2E2_4979 0.24 -1.2 5,745,349 - Pf6N2E2_4979 0.24 -0.6 5,745,349 - Pf6N2E2_4979 0.24 -2.6 5,745,349 - Pf6N2E2_4979 0.24 -0.9 5,745,349 - Pf6N2E2_4979 0.24 -0.6 5,745,349 - Pf6N2E2_4979 0.24 -0.6 5,745,351 + Pf6N2E2_4979 0.25 -1.4 5,745,559 - Pf6N2E2_4979 0.38 -0.8 5,745,559 - Pf6N2E2_4979 0.38 -0.6 5,745,559 - Pf6N2E2_4979 0.38 +1.4 5,746,053 + Pf6N2E2_4979 0.71 -0.2 5,746,384 + +0.4 5,746,384 + -0.8 5,746,384 + +0.4 5,746,384 + -0.6 5,746,384 + +0.5 5,746,385 - -1.2 5,746,385 - -0.2 5,746,385 - -0.6 5,746,385 - -0.6 5,746,385 - -0.6 5,746,385 - -0.4 5,746,385 - -0.6 5,746,385 - +0.4 5,746,385 - -0.6 5,746,385 - +0.4 5,746,385 - -1.6 5,746,395 + +0.4 5,746,395 + -0.6 5,746,395 + -0.6 5,746,395 + +0.4 5,746,395 + +0.4 5,746,396 - +0.4 5,746,396 - +0.4 5,746,396 - -2.9 5,746,396 - -1.6 5,746,434 + +0.4 5,746,434 + +0.7 5,746,434 + +0.8 5,746,434 + +0.4 5,746,434 + +0.4 5,746,435 - -0.2 5,746,435 - +0.4 5,746,435 - -1.1 5,746,435 - -1.2 5,746,435 - -0.7 5,746,435 - -0.6 5,746,438 + +0.4 5,746,443 + -5.0 5,746,443 + +1.5 5,746,444 - +0.4 5,746,444 - +0.4 5,746,444 - -1.6 5,746,444 - +0.4 5,746,465 + -0.6 5,746,525 + +1.0 5,746,525 + +0.4 5,746,525 + -0.6 5,746,526 - -0.6 5,746,526 - -0.6 5,746,527 + -0.6 5,746,527 + +0.4 5,746,527 + +0.4 5,746,528 - +0.4 5,746,528 - -1.9 5,746,528 - -1.6 5,746,562 + +0.4 5,746,562 + +1.6 5,746,562 + -1.2 5,746,563 - +0.4 5,746,563 - +0.4 5,746,563 - -0.6 5,746,568 + +0.4 5,746,784 + Pf6N2E2_4980 0.41 +1.4 5,746,784 + Pf6N2E2_4980 0.41 +0.4 5,746,784 + Pf6N2E2_4980 0.41 -0.6 5,746,785 - Pf6N2E2_4980 0.41 -0.8 5,746,786 + Pf6N2E2_4980 0.41 +0.4 5,746,786 + Pf6N2E2_4980 0.41 -0.6 5,746,786 + Pf6N2E2_4980 0.41 -0.2 5,746,786 + Pf6N2E2_4980 0.41 +1.9 5,746,786 + Pf6N2E2_4980 0.41 +0.4 5,746,786 + Pf6N2E2_4980 0.41 +1.4 5,746,787 - Pf6N2E2_4980 0.42 +0.4 5,746,956 + Pf6N2E2_4980 0.74 -1.6 5,746,957 - Pf6N2E2_4980 0.74 -1.2 5,746,957 - Pf6N2E2_4980 0.74 -0.1 5,746,957 - Pf6N2E2_4980 0.74 -1.1 5,747,059 + -0.6 5,747,059 + -0.6 5,747,062 + +0.4 5,747,062 + -0.9 5,747,063 - -0.6 5,747,063 - -0.6 5,747,063 - -0.6
Or see this region's nucleotide sequence