Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1704 and Pf6N2E2_1705 overlap by 4 nucleotides Pf6N2E2_1705 and Pf6N2E2_1706 overlap by 4 nucleotides Pf6N2E2_1706 and Pf6N2E2_1707 are separated by 4 nucleotides
Pf6N2E2_1704 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 2,012,909 to 2,013,790
_1704
Pf6N2E2_1705 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,013,787 to 2,014,557
_1705
Pf6N2E2_1706 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,014,554 to 2,015,525
_1706
Pf6N2E2_1707 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,015,530 to 2,016,915
_1707
Position (kb)
2014
2015
2016 Strain fitness (log2 ratio)
-2
-1
0
1 at 2013.705 kb on + strand at 2013.705 kb on + strand at 2013.705 kb on + strand at 2013.706 kb on - strand at 2013.706 kb on - strand at 2013.810 kb on - strand at 2013.875 kb on + strand, within Pf6N2E2_1705 at 2014.573 kb on + strand at 2014.574 kb on - strand at 2014.574 kb on - strand at 2014.611 kb on + strand at 2015.187 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.200 kb on - strand, within Pf6N2E2_1706 at 2015.515 kb on + strand at 2015.515 kb on + strand at 2015.516 kb on - strand at 2015.757 kb on - strand, within Pf6N2E2_1707 at 2016.036 kb on - strand, within Pf6N2E2_1707 at 2016.044 kb on + strand, within Pf6N2E2_1707 at 2016.044 kb on + strand, within Pf6N2E2_1707 at 2016.443 kb on + strand, within Pf6N2E2_1707 at 2016.444 kb on - strand, within Pf6N2E2_1707 at 2016.459 kb on - strand, within Pf6N2E2_1707
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 2,013,705 + -1.2 2,013,705 + -0.8 2,013,705 + -1.9 2,013,706 - -0.8 2,013,706 - +0.2 2,013,810 - -0.9 2,013,875 + Pf6N2E2_1705 0.11 -0.8 2,014,573 + +0.2 2,014,574 - -0.8 2,014,574 - -0.8 2,014,611 + -0.7 2,015,187 + Pf6N2E2_1706 0.65 +0.3 2,015,199 + Pf6N2E2_1706 0.66 +0.3 2,015,199 + Pf6N2E2_1706 0.66 -0.2 2,015,199 + Pf6N2E2_1706 0.66 +1.3 2,015,200 - Pf6N2E2_1706 0.66 -0.3 2,015,515 + +0.3 2,015,515 + +0.3 2,015,516 - -0.7 2,015,757 - Pf6N2E2_1707 0.16 +0.3 2,016,036 - Pf6N2E2_1707 0.37 +0.4 2,016,044 + Pf6N2E2_1707 0.37 +0.3 2,016,044 + Pf6N2E2_1707 0.37 +0.3 2,016,443 + Pf6N2E2_1707 0.66 +0.3 2,016,444 - Pf6N2E2_1707 0.66 +0.3 2,016,459 - Pf6N2E2_1707 0.67 +0.3
Or see this region's nucleotide sequence