Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_68 and Pf6N2E2_69 are separated by 210 nucleotides Pf6N2E2_69 and Pf6N2E2_70 are separated by 337 nucleotides
Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467
_68
Pf6N2E2_69 - Probable type IV pilus assembly FimV-related transmembrane protein, at 109,678 to 112,272
_69
Pf6N2E2_70 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 112,610 to 113,464
_70
Position (kb)
109
110
111
112
113 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.195 kb on - strand, within Pf6N2E2_68 at 109.333 kb on + strand, within Pf6N2E2_68 at 109.333 kb on + strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.377 kb on - strand at 109.377 kb on - strand at 109.377 kb on - strand at 109.380 kb on + strand at 109.380 kb on + strand at 109.380 kb on + strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.398 kb on + strand at 109.451 kb on + strand at 109.465 kb on + strand at 109.472 kb on + strand at 109.472 kb on + strand at 109.500 kb on + strand at 109.511 kb on + strand at 109.511 kb on + strand at 109.529 kb on + strand at 109.530 kb on - strand at 109.618 kb on - strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.678 kb on - strand at 109.703 kb on + strand at 109.703 kb on + strand at 109.704 kb on - strand at 109.704 kb on - strand at 109.709 kb on + strand at 109.710 kb on - strand at 110.051 kb on - strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.164 kb on + strand, within Pf6N2E2_69 at 110.164 kb on + strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.905 kb on + strand, within Pf6N2E2_69 at 110.905 kb on + strand, within Pf6N2E2_69 at 110.905 kb on + strand, within Pf6N2E2_69 at 111.382 kb on + strand, within Pf6N2E2_69 at 111.383 kb on - strand at 111.553 kb on + strand, within Pf6N2E2_69 at 111.553 kb on + strand, within Pf6N2E2_69 at 111.554 kb on - strand, within Pf6N2E2_69 at 111.554 kb on - strand, within Pf6N2E2_69 at 111.554 kb on - strand, within Pf6N2E2_69 at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.160 kb on - strand at 112.160 kb on - strand at 112.282 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.285 kb on - strand at 112.285 kb on - strand at 112.285 kb on - strand at 112.569 kb on + strand at 112.569 kb on + strand at 112.569 kb on + strand at 112.570 kb on - strand at 112.570 kb on - strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.589 kb on - strand at 112.589 kb on - strand at 112.603 kb on + strand at 112.620 kb on + strand at 112.620 kb on + strand at 112.621 kb on - strand at 112.682 kb on + strand at 112.683 kb on - strand at 112.991 kb on + strand, within Pf6N2E2_70 at 112.992 kb on - strand, within Pf6N2E2_70 at 112.992 kb on - strand, within Pf6N2E2_70 at 113.000 kb on + strand, within Pf6N2E2_70 at 113.000 kb on + strand, within Pf6N2E2_70 at 113.001 kb on - strand, within Pf6N2E2_70
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 109,084 - Pf6N2E2_68 0.62 +0.3 109,084 - Pf6N2E2_68 0.62 +0.3 109,195 - Pf6N2E2_68 0.73 -1.3 109,333 + Pf6N2E2_68 0.87 +0.3 109,333 + Pf6N2E2_68 0.87 +0.3 109,334 - Pf6N2E2_68 0.87 -0.7 109,334 - Pf6N2E2_68 0.87 +0.3 109,334 - Pf6N2E2_68 0.87 -0.5 109,376 + +0.3 109,376 + +0.3 109,376 + -0.7 109,376 + +0.3 109,376 + +0.3 109,377 - +0.3 109,377 - +0.3 109,377 - +0.3 109,380 + +0.3 109,380 + +0.3 109,380 + -0.4 109,381 - -0.7 109,381 - -0.3 109,381 - -0.3 109,381 - -0.2 109,398 + +0.3 109,451 + +0.3 109,465 + +0.3 109,472 + -0.6 109,472 + +0.3 109,500 + -0.7 109,511 + +0.3 109,511 + +0.3 109,529 + +0.3 109,530 - -0.7 109,618 - +0.3 109,634 + -0.7 109,634 + +0.3 109,634 + -1.3 109,634 + +0.3 109,678 - +0.3 109,703 + +0.3 109,703 + +0.3 109,704 - +0.3 109,704 - -2.8 109,709 + +0.3 109,710 - +0.3 110,051 - Pf6N2E2_69 0.14 +0.3 110,057 + Pf6N2E2_69 0.15 -0.7 110,057 + Pf6N2E2_69 0.15 +0.3 110,057 + Pf6N2E2_69 0.15 -2.9 110,058 - Pf6N2E2_69 0.15 +1.0 110,058 - Pf6N2E2_69 0.15 +0.5 110,058 - Pf6N2E2_69 0.15 +0.3 110,164 + Pf6N2E2_69 0.19 +0.3 110,164 + Pf6N2E2_69 0.19 +0.7 110,165 - Pf6N2E2_69 0.19 +0.7 110,165 - Pf6N2E2_69 0.19 +0.3 110,165 - Pf6N2E2_69 0.19 +0.3 110,905 + Pf6N2E2_69 0.47 +0.6 110,905 + Pf6N2E2_69 0.47 +0.7 110,905 + Pf6N2E2_69 0.47 -2.0 111,382 + Pf6N2E2_69 0.66 +0.3 111,383 - +0.3 111,553 + Pf6N2E2_69 0.72 -0.7 111,553 + Pf6N2E2_69 0.72 +0.3 111,554 - Pf6N2E2_69 0.72 +0.5 111,554 - Pf6N2E2_69 0.72 -1.3 111,554 - Pf6N2E2_69 0.72 +0.7 112,159 + -0.3 112,159 + -0.7 112,159 + +0.6 112,159 + +0.6 112,159 + +1.0 112,159 + +0.7 112,159 + -0.1 112,160 - +0.5 112,160 - +0.6 112,282 + +0.3 112,284 + -0.9 112,284 + +0.3 112,284 + -0.2 112,284 + +0.2 112,284 + -0.4 112,284 + +0.3 112,285 - -0.7 112,285 - +0.3 112,285 - -1.0 112,569 + -1.3 112,569 + +0.3 112,569 + -1.1 112,570 - -0.6 112,570 - -1.5 112,571 + -1.3 112,571 + -1.3 112,571 + -0.7 112,571 + +0.3 112,571 + -0.7 112,571 + -0.9 112,571 + -0.1 112,572 - +0.3 112,572 - +1.3 112,572 - -2.1 112,572 - -2.3 112,589 - +0.3 112,589 - -2.0 112,603 + -0.7 112,620 + +0.3 112,620 + -2.0 112,621 - -1.3 112,682 + +0.3 112,683 - +0.3 112,991 + Pf6N2E2_70 0.45 +0.3 112,992 - Pf6N2E2_70 0.45 +0.3 112,992 - Pf6N2E2_70 0.45 -1.7 113,000 + Pf6N2E2_70 0.46 -1.3 113,000 + Pf6N2E2_70 0.46 -2.4 113,001 - Pf6N2E2_70 0.46 +0.3
Or see this region's nucleotide sequence