Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_630

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotidesPf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591 _629 Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366 _630 Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455 _631 Position (kb) 717 718 719Strain fitness (log2 ratio) -1 0 1at 716.888 kb on + strand, within Pf6N2E2_629at 716.889 kb on - strand, within Pf6N2E2_629at 716.889 kb on - strand, within Pf6N2E2_629at 717.272 kb on + strand, within Pf6N2E2_629at 717.272 kb on + strand, within Pf6N2E2_629at 717.272 kb on + strand, within Pf6N2E2_629at 717.698 kb on + strand, within Pf6N2E2_630at 717.699 kb on - strand, within Pf6N2E2_630at 717.813 kb on + strand, within Pf6N2E2_630at 717.813 kb on + strand, within Pf6N2E2_630at 717.813 kb on + strand, within Pf6N2E2_630at 717.814 kb on - strand, within Pf6N2E2_630at 717.814 kb on - strand, within Pf6N2E2_630at 718.071 kb on - strand, within Pf6N2E2_630at 718.370 kb on + strandat 718.370 kb on + strandat 718.370 kb on + strandat 718.370 kb on + strandat 718.370 kb on + strandat 718.371 kb on - strandat 718.371 kb on - strandat 718.373 kb on + strandat 718.373 kb on + strandat 718.634 kb on + strand, within Pf6N2E2_631at 718.634 kb on + strand, within Pf6N2E2_631at 718.635 kb on - strand, within Pf6N2E2_631at 718.635 kb on - strand, within Pf6N2E2_631at 719.083 kb on - strand, within Pf6N2E2_631at 719.083 kb on - strand, within Pf6N2E2_631at 719.083 kb on - strand, within Pf6N2E2_631at 719.152 kb on + strand, within Pf6N2E2_631at 719.152 kb on + strand, within Pf6N2E2_631at 719.153 kb on - strand, within Pf6N2E2_631at 719.153 kb on - strand, within Pf6N2E2_631at 719.153 kb on - strand, within Pf6N2E2_631

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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716,888 + Pf6N2E2_629 0.28 +0.3
716,889 - Pf6N2E2_629 0.28 +0.3
716,889 - Pf6N2E2_629 0.28 -0.7
717,272 + Pf6N2E2_629 0.67 -0.4
717,272 + Pf6N2E2_629 0.67 +0.3
717,272 + Pf6N2E2_629 0.67 +0.2
717,698 + Pf6N2E2_630 0.15 +0.3
717,699 - Pf6N2E2_630 0.15 +0.4
717,813 + Pf6N2E2_630 0.29 +0.3
717,813 + Pf6N2E2_630 0.29 +0.3
717,813 + Pf6N2E2_630 0.29 +0.3
717,814 - Pf6N2E2_630 0.29 +0.3
717,814 - Pf6N2E2_630 0.29 +0.3
718,071 - Pf6N2E2_630 0.62 -0.7
718,370 + +0.3
718,370 + +0.3
718,370 + +0.3
718,370 + -0.7
718,370 + -1.3
718,371 - -0.8
718,371 - -0.7
718,373 + -0.7
718,373 + +0.3
718,634 + Pf6N2E2_631 0.20 -0.3
718,634 + Pf6N2E2_631 0.20 +0.3
718,635 - Pf6N2E2_631 0.20 -0.6
718,635 - Pf6N2E2_631 0.20 -1.3
719,083 - Pf6N2E2_631 0.64 -0.3
719,083 - Pf6N2E2_631 0.64 +0.3
719,083 - Pf6N2E2_631 0.64 +0.3
719,152 + Pf6N2E2_631 0.70 -1.7
719,152 + Pf6N2E2_631 0.70 +0.3
719,153 - Pf6N2E2_631 0.70 -0.7
719,153 - Pf6N2E2_631 0.70 +0.3
719,153 - Pf6N2E2_631 0.70 +0.7

Or see this region's nucleotide sequence