Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_401 and Pf6N2E2_402 are separated by 97 nucleotides Pf6N2E2_402 and Pf6N2E2_403 are separated by 39 nucleotides Pf6N2E2_403 and Pf6N2E2_404 are separated by 244 nucleotides
Pf6N2E2_401 - Cyn operon transcriptional activator, at 471,321 to 472,220
_401
Pf6N2E2_402 - Carbonic anhydrase (EC 4.2.1.1), at 472,318 to 472,977
_402
Pf6N2E2_403 - Cyanate hydratase (EC 4.2.1.104), at 473,017 to 473,487
_403
Pf6N2E2_404 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 473,732 to 473,854
_404
Position (kb) 473
474 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.186 kb on + strand at 472.187 kb on - strand at 472.187 kb on - strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.280 kb on - strand at 472.280 kb on - strand at 472.280 kb on - strand at 472.601 kb on + strand, within Pf6N2E2_402 at 472.602 kb on - strand, within Pf6N2E2_402 at 472.751 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.985 kb on - strand at 473.012 kb on - strand at 473.299 kb on + strand, within Pf6N2E2_403 at 473.299 kb on + strand, within Pf6N2E2_403 at 473.299 kb on + strand, within Pf6N2E2_403 at 473.300 kb on - strand, within Pf6N2E2_403 at 473.300 kb on - strand, within Pf6N2E2_403 at 473.309 kb on - strand, within Pf6N2E2_403 at 473.309 kb on - strand, within Pf6N2E2_403 at 473.327 kb on - strand, within Pf6N2E2_403 at 473.327 kb on - strand, within Pf6N2E2_403 at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.465 kb on - strand at 473.465 kb on - strand at 473.465 kb on - strand at 473.472 kb on + strand at 473.473 kb on - strand at 473.490 kb on + strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.523 kb on - strand at 473.523 kb on - strand at 473.540 kb on + strand at 473.540 kb on + strand at 473.586 kb on + strand at 473.586 kb on + strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.620 kb on - strand at 473.620 kb on - strand at 473.620 kb on - strand at 473.665 kb on + strand at 473.665 kb on + strand at 473.666 kb on - strand at 473.684 kb on - strand at 473.755 kb on + strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.767 kb on - strand, within Pf6N2E2_404 at 473.787 kb on - strand, within Pf6N2E2_404 at 473.787 kb on - strand, within Pf6N2E2_404 at 473.823 kb on - strand, within Pf6N2E2_404 at 473.830 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.836 kb on + strand, within Pf6N2E2_404 at 473.944 kb on + strand at 473.944 kb on + strand at 473.945 kb on - strand at 473.947 kb on + strand at 473.947 kb on + strand at 473.948 kb on - strand at 473.948 kb on - strand at 473.948 kb on - strand at 473.989 kb on - strand at 473.989 kb on - strand at 473.989 kb on - strand at 473.994 kb on + strand at 473.995 kb on - strand at 474.000 kb on - strand at 474.125 kb on - strand at 474.164 kb on + strand at 474.164 kb on + strand at 474.165 kb on - strand at 474.165 kb on - strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.237 kb on - strand at 474.312 kb on + strand at 474.313 kb on - strand at 474.314 kb on + strand at 474.329 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.389 kb on - strand at 474.475 kb on + strand at 474.475 kb on + strand at 474.475 kb on + strand at 474.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 472,018 + Pf6N2E2_401 0.77 +0.3 472,018 + Pf6N2E2_401 0.77 +0.3 472,018 + Pf6N2E2_401 0.77 +0.9 472,019 - Pf6N2E2_401 0.78 -0.1 472,019 - Pf6N2E2_401 0.78 +0.3 472,019 - Pf6N2E2_401 0.78 +0.3 472,186 + +0.3 472,187 - -0.9 472,187 - +0.3 472,279 + -1.3 472,279 + -0.7 472,279 + +0.3 472,279 + +0.3 472,280 - +0.3 472,280 - -2.5 472,280 - +0.3 472,601 + Pf6N2E2_402 0.43 +0.3 472,602 - Pf6N2E2_402 0.43 +0.3 472,751 + Pf6N2E2_402 0.66 -0.7 472,861 + Pf6N2E2_402 0.82 +0.3 472,861 + Pf6N2E2_402 0.82 -0.7 472,862 - Pf6N2E2_402 0.82 +0.3 472,862 - Pf6N2E2_402 0.82 -1.5 472,862 - Pf6N2E2_402 0.82 +0.3 472,862 - Pf6N2E2_402 0.82 +0.3 472,862 - Pf6N2E2_402 0.82 -0.7 472,985 - -0.3 473,012 - -1.7 473,299 + Pf6N2E2_403 0.60 -0.7 473,299 + Pf6N2E2_403 0.60 +0.3 473,299 + Pf6N2E2_403 0.60 +0.3 473,300 - Pf6N2E2_403 0.60 +0.3 473,300 - Pf6N2E2_403 0.60 +0.3 473,309 - Pf6N2E2_403 0.62 +0.3 473,309 - Pf6N2E2_403 0.62 -0.7 473,327 - Pf6N2E2_403 0.66 +0.3 473,327 - Pf6N2E2_403 0.66 +1.3 473,464 + -1.3 473,464 + +0.8 473,464 + -0.7 473,464 + -1.3 473,464 + +0.3 473,464 + +0.1 473,464 + +0.3 473,465 - +0.3 473,465 - +0.3 473,465 - +0.3 473,472 + -2.5 473,473 - -0.7 473,490 + +0.3 473,491 - -0.4 473,491 - +0.3 473,491 - +0.3 473,491 - +0.3 473,523 - -4.0 473,523 - +0.3 473,540 + -0.7 473,540 + -0.7 473,586 + +0.3 473,586 + -1.3 473,587 - -1.3 473,587 - +0.3 473,587 - +0.3 473,587 - +0.3 473,620 - +0.3 473,620 - +0.6 473,620 - -0.7 473,665 + +1.2 473,665 + -0.1 473,666 - +0.3 473,684 - +0.3 473,755 + Pf6N2E2_404 0.19 -0.7 473,756 - Pf6N2E2_404 0.20 +0.3 473,756 - Pf6N2E2_404 0.20 +0.3 473,756 - Pf6N2E2_404 0.20 +0.3 473,766 + Pf6N2E2_404 0.28 +0.3 473,766 + Pf6N2E2_404 0.28 -0.7 473,766 + Pf6N2E2_404 0.28 +0.3 473,767 - Pf6N2E2_404 0.28 -0.3 473,787 - Pf6N2E2_404 0.45 +0.3 473,787 - Pf6N2E2_404 0.45 +0.3 473,823 - Pf6N2E2_404 0.74 -0.7 473,830 + Pf6N2E2_404 0.80 -0.8 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.9 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 - Pf6N2E2_404 0.81 -0.5 473,833 - Pf6N2E2_404 0.82 +0.3 473,833 - Pf6N2E2_404 0.82 +0.3 473,833 - Pf6N2E2_404 0.82 +0.3 473,833 - Pf6N2E2_404 0.82 +0.3 473,833 - Pf6N2E2_404 0.82 +0.3 473,836 + Pf6N2E2_404 0.85 -1.3 473,944 + +1.3 473,944 + -0.7 473,945 - -0.7 473,947 + +0.3 473,947 + +0.3 473,948 - -2.0 473,948 - +0.3 473,948 - +0.3 473,989 - +0.3 473,989 - +0.3 473,989 - +0.3 473,994 + -0.1 473,995 - +0.3 474,000 - -0.7 474,125 - -0.9 474,164 + -0.5 474,164 + -0.7 474,165 - -1.8 474,165 - +0.3 474,236 + +0.3 474,236 + +0.3 474,236 + +0.3 474,236 + -0.7 474,237 - +0.9 474,312 + +0.3 474,313 - +0.3 474,314 + -0.7 474,329 + +0.3 474,388 + -0.7 474,388 + +0.3 474,388 + +0.0 474,388 + -0.7 474,389 - -0.7 474,475 + -1.3 474,475 + -1.9 474,475 + +0.3 474,476 - +0.3
Or see this region's nucleotide sequence