Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3560 and Pf6N2E2_3561 are separated by 218 nucleotides Pf6N2E2_3561 and Pf6N2E2_3562 are separated by 137 nucleotides Pf6N2E2_3562 and Pf6N2E2_3563 are separated by 118 nucleotides Pf6N2E2_3563 and Pf6N2E2_3564 are separated by 136 nucleotides
Pf6N2E2_3560 - membrane protein, putative, at 4,151,802 to 4,152,815
_3560
Pf6N2E2_3561 - Phosphate starvation-inducible protein psiF precursor, at 4,153,034 to 4,153,321
_3561
Pf6N2E2_3562 - Lipase precursor (EC 3.1.1.3), at 4,153,459 to 4,154,349
_3562
Pf6N2E2_3563 - FMN-dependent NADH-azoreductase, at 4,154,468 to 4,155,106
_3563
Pf6N2E2_3564 - Glycine cleavage system transcriptional activator, at 4,155,243 to 4,156,184
_3564
Position (kb)
4153
4154
4155 Strain fitness (log2 ratio)
-1
0
1
2 at 4152.460 kb on + strand, within Pf6N2E2_3560 at 4152.460 kb on + strand, within Pf6N2E2_3560 at 4152.460 kb on + strand, within Pf6N2E2_3560 at 4152.461 kb on - strand, within Pf6N2E2_3560 at 4152.461 kb on - strand, within Pf6N2E2_3560 at 4152.815 kb on + strand at 4152.815 kb on + strand at 4152.815 kb on + strand at 4152.816 kb on - strand at 4152.816 kb on - strand at 4152.816 kb on - strand at 4152.816 kb on - strand at 4152.887 kb on + strand at 4152.887 kb on + strand at 4152.887 kb on + strand at 4152.921 kb on + strand at 4153.036 kb on - strand at 4153.124 kb on - strand, within Pf6N2E2_3561 at 4153.124 kb on - strand, within Pf6N2E2_3561 at 4153.280 kb on + strand, within Pf6N2E2_3561 at 4153.281 kb on - strand, within Pf6N2E2_3561 at 4153.370 kb on + strand at 4153.371 kb on - strand at 4153.384 kb on + strand at 4153.384 kb on + strand at 4153.384 kb on + strand at 4153.385 kb on - strand at 4153.385 kb on - strand at 4153.385 kb on - strand at 4153.385 kb on - strand at 4153.461 kb on - strand at 4153.610 kb on + strand, within Pf6N2E2_3562 at 4153.666 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.748 kb on + strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.749 kb on - strand, within Pf6N2E2_3562 at 4153.751 kb on + strand, within Pf6N2E2_3562 at 4153.873 kb on - strand, within Pf6N2E2_3562 at 4153.998 kb on - strand, within Pf6N2E2_3562 at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.273 kb on + strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.274 kb on - strand at 4154.288 kb on - strand at 4154.314 kb on + strand at 4154.315 kb on - strand at 4154.404 kb on + strand at 4154.404 kb on + strand at 4154.404 kb on + strand at 4154.404 kb on + strand at 4154.405 kb on - strand at 4154.448 kb on - strand at 4154.715 kb on + strand, within Pf6N2E2_3563 at 4154.925 kb on + strand, within Pf6N2E2_3563 at 4154.925 kb on + strand, within Pf6N2E2_3563 at 4154.967 kb on - strand, within Pf6N2E2_3563 at 4154.967 kb on - strand, within Pf6N2E2_3563 at 4155.100 kb on + strand at 4155.100 kb on + strand at 4155.101 kb on - strand at 4155.128 kb on + strand at 4155.144 kb on + strand at 4155.144 kb on + strand at 4155.144 kb on + strand at 4155.145 kb on - strand at 4155.193 kb on - strand at 4155.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 4,152,460 + Pf6N2E2_3560 0.65 +0.5 4,152,460 + Pf6N2E2_3560 0.65 +0.1 4,152,460 + Pf6N2E2_3560 0.65 -0.1 4,152,461 - Pf6N2E2_3560 0.65 +0.5 4,152,461 - Pf6N2E2_3560 0.65 -0.5 4,152,815 + -1.5 4,152,815 + +0.5 4,152,815 + +0.5 4,152,816 - -0.5 4,152,816 - -0.5 4,152,816 - -1.5 4,152,816 - +0.5 4,152,887 + -0.9 4,152,887 + +0.5 4,152,887 + -0.5 4,152,921 + +0.7 4,153,036 - -1.1 4,153,124 - Pf6N2E2_3561 0.31 -0.3 4,153,124 - Pf6N2E2_3561 0.31 -0.3 4,153,280 + Pf6N2E2_3561 0.85 +0.5 4,153,281 - Pf6N2E2_3561 0.86 -0.5 4,153,370 + +0.5 4,153,371 - +0.5 4,153,384 + +0.5 4,153,384 + +0.5 4,153,384 + -0.9 4,153,385 - +0.5 4,153,385 - +0.5 4,153,385 - -0.5 4,153,385 - -0.5 4,153,461 - -0.4 4,153,610 + Pf6N2E2_3562 0.17 -1.5 4,153,666 + Pf6N2E2_3562 0.23 +0.1 4,153,748 + Pf6N2E2_3562 0.32 +2.1 4,153,748 + Pf6N2E2_3562 0.32 +0.5 4,153,748 + Pf6N2E2_3562 0.32 +0.5 4,153,748 + Pf6N2E2_3562 0.32 +0.5 4,153,748 + Pf6N2E2_3562 0.32 -0.7 4,153,749 - Pf6N2E2_3562 0.33 -0.5 4,153,749 - Pf6N2E2_3562 0.33 +1.1 4,153,749 - Pf6N2E2_3562 0.33 -0.5 4,153,749 - Pf6N2E2_3562 0.33 +0.5 4,153,749 - Pf6N2E2_3562 0.33 +0.2 4,153,749 - Pf6N2E2_3562 0.33 -0.5 4,153,751 + Pf6N2E2_3562 0.33 -1.1 4,153,873 - Pf6N2E2_3562 0.46 +0.5 4,153,998 - Pf6N2E2_3562 0.60 -0.0 4,154,273 + +0.5 4,154,273 + +0.5 4,154,273 + +0.5 4,154,273 + +0.7 4,154,273 + +0.5 4,154,274 - +0.5 4,154,274 - +0.5 4,154,274 - +0.5 4,154,274 - +0.5 4,154,274 - +0.5 4,154,274 - -0.5 4,154,274 - -0.1 4,154,274 - -0.5 4,154,274 - +0.5 4,154,288 - -0.5 4,154,314 + -0.5 4,154,315 - +0.5 4,154,404 + -0.4 4,154,404 + -0.5 4,154,404 + -0.5 4,154,404 + +0.5 4,154,405 - +0.5 4,154,448 - -1.1 4,154,715 + Pf6N2E2_3563 0.39 -0.7 4,154,925 + Pf6N2E2_3563 0.72 -0.3 4,154,925 + Pf6N2E2_3563 0.72 -1.5 4,154,967 - Pf6N2E2_3563 0.78 +0.5 4,154,967 - Pf6N2E2_3563 0.78 +0.5 4,155,100 + -0.5 4,155,100 + -0.5 4,155,101 - +0.5 4,155,128 + -0.5 4,155,144 + -1.5 4,155,144 + +0.5 4,155,144 + +0.5 4,155,145 - +0.5 4,155,193 - +0.5 4,155,211 - +0.5
Or see this region's nucleotide sequence