Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3562

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3560 and Pf6N2E2_3561 are separated by 218 nucleotidesPf6N2E2_3561 and Pf6N2E2_3562 are separated by 137 nucleotidesPf6N2E2_3562 and Pf6N2E2_3563 are separated by 118 nucleotidesPf6N2E2_3563 and Pf6N2E2_3564 are separated by 136 nucleotides Pf6N2E2_3560 - membrane protein, putative, at 4,151,802 to 4,152,815 _3560 Pf6N2E2_3561 - Phosphate starvation-inducible protein psiF precursor, at 4,153,034 to 4,153,321 _3561 Pf6N2E2_3562 - Lipase precursor (EC 3.1.1.3), at 4,153,459 to 4,154,349 _3562 Pf6N2E2_3563 - FMN-dependent NADH-azoreductase, at 4,154,468 to 4,155,106 _3563 Pf6N2E2_3564 - Glycine cleavage system transcriptional activator, at 4,155,243 to 4,156,184 _3564 Position (kb) 4153 4154 4155Strain fitness (log2 ratio) -1 0 1 2at 4152.460 kb on + strand, within Pf6N2E2_3560at 4152.460 kb on + strand, within Pf6N2E2_3560at 4152.460 kb on + strand, within Pf6N2E2_3560at 4152.461 kb on - strand, within Pf6N2E2_3560at 4152.461 kb on - strand, within Pf6N2E2_3560at 4152.815 kb on + strandat 4152.815 kb on + strandat 4152.815 kb on + strandat 4152.816 kb on - strandat 4152.816 kb on - strandat 4152.816 kb on - strandat 4152.816 kb on - strandat 4152.887 kb on + strandat 4152.887 kb on + strandat 4152.887 kb on + strandat 4152.921 kb on + strandat 4153.036 kb on - strandat 4153.124 kb on - strand, within Pf6N2E2_3561at 4153.124 kb on - strand, within Pf6N2E2_3561at 4153.280 kb on + strand, within Pf6N2E2_3561at 4153.281 kb on - strand, within Pf6N2E2_3561at 4153.370 kb on + strandat 4153.371 kb on - strandat 4153.384 kb on + strandat 4153.384 kb on + strandat 4153.384 kb on + strandat 4153.385 kb on - strandat 4153.385 kb on - strandat 4153.385 kb on - strandat 4153.385 kb on - strandat 4153.461 kb on - strandat 4153.610 kb on + strand, within Pf6N2E2_3562at 4153.666 kb on + strand, within Pf6N2E2_3562at 4153.748 kb on + strand, within Pf6N2E2_3562at 4153.748 kb on + strand, within Pf6N2E2_3562at 4153.748 kb on + strand, within Pf6N2E2_3562at 4153.748 kb on + strand, within Pf6N2E2_3562at 4153.748 kb on + strand, within Pf6N2E2_3562at 4153.749 kb on - strand, within Pf6N2E2_3562at 4153.749 kb on - strand, within Pf6N2E2_3562at 4153.749 kb on - strand, within Pf6N2E2_3562at 4153.749 kb on - strand, within Pf6N2E2_3562at 4153.749 kb on - strand, within Pf6N2E2_3562at 4153.749 kb on - strand, within Pf6N2E2_3562at 4153.751 kb on + strand, within Pf6N2E2_3562at 4153.873 kb on - strand, within Pf6N2E2_3562at 4153.998 kb on - strand, within Pf6N2E2_3562at 4154.273 kb on + strandat 4154.273 kb on + strandat 4154.273 kb on + strandat 4154.273 kb on + strandat 4154.273 kb on + strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.274 kb on - strandat 4154.288 kb on - strandat 4154.314 kb on + strandat 4154.315 kb on - strandat 4154.404 kb on + strandat 4154.404 kb on + strandat 4154.404 kb on + strandat 4154.404 kb on + strandat 4154.405 kb on - strandat 4154.448 kb on - strandat 4154.715 kb on + strand, within Pf6N2E2_3563at 4154.925 kb on + strand, within Pf6N2E2_3563at 4154.925 kb on + strand, within Pf6N2E2_3563at 4154.967 kb on - strand, within Pf6N2E2_3563at 4154.967 kb on - strand, within Pf6N2E2_3563at 4155.100 kb on + strandat 4155.100 kb on + strandat 4155.101 kb on - strandat 4155.128 kb on + strandat 4155.144 kb on + strandat 4155.144 kb on + strandat 4155.144 kb on + strandat 4155.145 kb on - strandat 4155.193 kb on - strandat 4155.211 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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4,152,460 + Pf6N2E2_3560 0.65 +0.5
4,152,460 + Pf6N2E2_3560 0.65 +0.1
4,152,460 + Pf6N2E2_3560 0.65 -0.1
4,152,461 - Pf6N2E2_3560 0.65 +0.5
4,152,461 - Pf6N2E2_3560 0.65 -0.5
4,152,815 + -1.5
4,152,815 + +0.5
4,152,815 + +0.5
4,152,816 - -0.5
4,152,816 - -0.5
4,152,816 - -1.5
4,152,816 - +0.5
4,152,887 + -0.9
4,152,887 + +0.5
4,152,887 + -0.5
4,152,921 + +0.7
4,153,036 - -1.1
4,153,124 - Pf6N2E2_3561 0.31 -0.3
4,153,124 - Pf6N2E2_3561 0.31 -0.3
4,153,280 + Pf6N2E2_3561 0.85 +0.5
4,153,281 - Pf6N2E2_3561 0.86 -0.5
4,153,370 + +0.5
4,153,371 - +0.5
4,153,384 + +0.5
4,153,384 + +0.5
4,153,384 + -0.9
4,153,385 - +0.5
4,153,385 - +0.5
4,153,385 - -0.5
4,153,385 - -0.5
4,153,461 - -0.4
4,153,610 + Pf6N2E2_3562 0.17 -1.5
4,153,666 + Pf6N2E2_3562 0.23 +0.1
4,153,748 + Pf6N2E2_3562 0.32 +2.1
4,153,748 + Pf6N2E2_3562 0.32 +0.5
4,153,748 + Pf6N2E2_3562 0.32 +0.5
4,153,748 + Pf6N2E2_3562 0.32 +0.5
4,153,748 + Pf6N2E2_3562 0.32 -0.7
4,153,749 - Pf6N2E2_3562 0.33 -0.5
4,153,749 - Pf6N2E2_3562 0.33 +1.1
4,153,749 - Pf6N2E2_3562 0.33 -0.5
4,153,749 - Pf6N2E2_3562 0.33 +0.5
4,153,749 - Pf6N2E2_3562 0.33 +0.2
4,153,749 - Pf6N2E2_3562 0.33 -0.5
4,153,751 + Pf6N2E2_3562 0.33 -1.1
4,153,873 - Pf6N2E2_3562 0.46 +0.5
4,153,998 - Pf6N2E2_3562 0.60 -0.0
4,154,273 + +0.5
4,154,273 + +0.5
4,154,273 + +0.5
4,154,273 + +0.7
4,154,273 + +0.5
4,154,274 - +0.5
4,154,274 - +0.5
4,154,274 - +0.5
4,154,274 - +0.5
4,154,274 - +0.5
4,154,274 - -0.5
4,154,274 - -0.1
4,154,274 - -0.5
4,154,274 - +0.5
4,154,288 - -0.5
4,154,314 + -0.5
4,154,315 - +0.5
4,154,404 + -0.4
4,154,404 + -0.5
4,154,404 + -0.5
4,154,404 + +0.5
4,154,405 - +0.5
4,154,448 - -1.1
4,154,715 + Pf6N2E2_3563 0.39 -0.7
4,154,925 + Pf6N2E2_3563 0.72 -0.3
4,154,925 + Pf6N2E2_3563 0.72 -1.5
4,154,967 - Pf6N2E2_3563 0.78 +0.5
4,154,967 - Pf6N2E2_3563 0.78 +0.5
4,155,100 + -0.5
4,155,100 + -0.5
4,155,101 - +0.5
4,155,128 + -0.5
4,155,144 + -1.5
4,155,144 + +0.5
4,155,144 + +0.5
4,155,145 - +0.5
4,155,193 - +0.5
4,155,211 - +0.5

Or see this region's nucleotide sequence