Experiment: Chloramphenicol 12.5 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3675 and Atu3676 overlap by 4 nucleotides Atu3676 and Atu3677 overlap by 4 nucleotides Atu3677 and Atu3678 are separated by 14 nucleotides Atu3678 and Atu3679 are separated by 10 nucleotides Atu3679 and Atu3680 overlap by 4 nucleotides
Atu3675: Atu3675 - peptide synthetase, siderophore biosynthesis protein, at 729,769 to 734,331
Atu3675
Atu3676: Atu3676 - siderophore biosynthesis protein, at 734,328 to 735,224
Atu3676
Atu3677: Atu3677 - polyketide synthase, at 735,221 to 736,696
Atu3677
Atu3678: Atu3678 - siderophore biosynthesis protein, at 736,711 to 736,950
Atu3678
Atu3679: Atu3679 - siderophore biosynthesis protein, at 736,961 to 737,479
Atu3679
Atu3680: Atu3680 - siderophore biosynthesis protein, at 737,476 to 738,039
Atu3680
Position (kb)
735
736
737 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 734.237 kb on + strand at 734.335 kb on + strand at 734.335 kb on + strand at 734.337 kb on + strand at 734.343 kb on - strand at 734.386 kb on + strand at 734.400 kb on - strand at 734.464 kb on - strand, within Atu3676 at 734.464 kb on - strand, within Atu3676 at 734.518 kb on - strand, within Atu3676 at 734.637 kb on - strand, within Atu3676 at 734.663 kb on - strand, within Atu3676 at 734.663 kb on - strand, within Atu3676 at 734.692 kb on + strand, within Atu3676 at 734.717 kb on - strand, within Atu3676 at 734.797 kb on - strand, within Atu3676 at 734.810 kb on - strand, within Atu3676 at 734.889 kb on + strand, within Atu3676 at 734.906 kb on + strand, within Atu3676 at 734.940 kb on + strand, within Atu3676 at 735.046 kb on - strand, within Atu3676 at 735.068 kb on - strand, within Atu3676 at 735.161 kb on - strand at 735.161 kb on - strand at 735.192 kb on + strand at 735.200 kb on - strand at 735.264 kb on - strand at 735.455 kb on - strand, within Atu3677 at 735.537 kb on + strand, within Atu3677 at 735.563 kb on - strand, within Atu3677 at 735.604 kb on + strand, within Atu3677 at 735.711 kb on - strand, within Atu3677 at 735.752 kb on + strand, within Atu3677 at 735.752 kb on + strand, within Atu3677 at 735.760 kb on - strand, within Atu3677 at 735.817 kb on - strand, within Atu3677 at 735.817 kb on - strand, within Atu3677 at 735.927 kb on - strand, within Atu3677 at 735.960 kb on - strand, within Atu3677 at 735.966 kb on + strand, within Atu3677 at 736.006 kb on - strand, within Atu3677 at 736.006 kb on - strand, within Atu3677 at 736.117 kb on + strand, within Atu3677 at 736.217 kb on + strand, within Atu3677 at 736.346 kb on + strand, within Atu3677 at 736.369 kb on + strand, within Atu3677 at 736.555 kb on - strand at 736.555 kb on - strand at 736.562 kb on + strand at 736.568 kb on - strand at 736.699 kb on + strand at 736.728 kb on + strand at 736.743 kb on + strand, within Atu3678 at 736.750 kb on + strand, within Atu3678 at 736.768 kb on + strand, within Atu3678 at 736.791 kb on + strand, within Atu3678 at 736.797 kb on - strand, within Atu3678 at 736.848 kb on - strand, within Atu3678 at 737.022 kb on + strand, within Atu3679 at 737.024 kb on - strand, within Atu3679 at 737.027 kb on + strand, within Atu3679 at 737.050 kb on + strand, within Atu3679 at 737.149 kb on + strand, within Atu3679 at 737.342 kb on - strand, within Atu3679 at 737.361 kb on - strand, within Atu3679 at 737.467 kb on - strand at 737.469 kb on + strand at 737.475 kb on - strand at 737.557 kb on - strand, within Atu3680 at 737.557 kb on - strand, within Atu3680 at 737.610 kb on + strand, within Atu3680 at 737.648 kb on + strand, within Atu3680
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 12.5 ug/mL remove 734,237 + +1.2 734,335 + +0.0 734,335 + -0.8 734,337 + -1.5 734,343 - -1.2 734,386 + -1.6 734,400 - -0.4 734,464 - Atu3676 0.15 -0.2 734,464 - Atu3676 0.15 +0.2 734,518 - Atu3676 0.21 -0.8 734,637 - Atu3676 0.34 +0.3 734,663 - Atu3676 0.37 -2.3 734,663 - Atu3676 0.37 +0.8 734,692 + Atu3676 0.41 -0.5 734,717 - Atu3676 0.43 +0.5 734,797 - Atu3676 0.52 +0.1 734,810 - Atu3676 0.54 +0.7 734,889 + Atu3676 0.63 +0.3 734,906 + Atu3676 0.64 +0.5 734,940 + Atu3676 0.68 -0.4 735,046 - Atu3676 0.80 +0.6 735,068 - Atu3676 0.82 +0.0 735,161 - +0.4 735,161 - +0.9 735,192 + -0.5 735,200 - -0.3 735,264 - -0.4 735,455 - Atu3677 0.16 +0.6 735,537 + Atu3677 0.21 -1.0 735,563 - Atu3677 0.23 -0.1 735,604 + Atu3677 0.26 -1.2 735,711 - Atu3677 0.33 +3.4 735,752 + Atu3677 0.36 +0.0 735,752 + Atu3677 0.36 -0.0 735,760 - Atu3677 0.37 -1.0 735,817 - Atu3677 0.40 +0.7 735,817 - Atu3677 0.40 -0.2 735,927 - Atu3677 0.48 +1.2 735,960 - Atu3677 0.50 -1.8 735,966 + Atu3677 0.50 -0.4 736,006 - Atu3677 0.53 +2.6 736,006 - Atu3677 0.53 +0.0 736,117 + Atu3677 0.61 -0.0 736,217 + Atu3677 0.67 -1.6 736,346 + Atu3677 0.76 +0.8 736,369 + Atu3677 0.78 -0.7 736,555 - -0.4 736,555 - +0.4 736,562 + -0.2 736,568 - +0.0 736,699 + +0.5 736,728 + +1.3 736,743 + Atu3678 0.13 +0.6 736,750 + Atu3678 0.16 +1.4 736,768 + Atu3678 0.24 +1.0 736,791 + Atu3678 0.33 +1.6 736,797 - Atu3678 0.36 -0.4 736,848 - Atu3678 0.57 -1.3 737,022 + Atu3679 0.12 +0.1 737,024 - Atu3679 0.12 +0.6 737,027 + Atu3679 0.13 -0.5 737,050 + Atu3679 0.17 +0.3 737,149 + Atu3679 0.36 +1.1 737,342 - Atu3679 0.73 -0.4 737,361 - Atu3679 0.77 -0.7 737,467 - +0.1 737,469 + -0.2 737,475 - -1.0 737,557 - Atu3680 0.14 -1.0 737,557 - Atu3680 0.14 +0.3 737,610 + Atu3680 0.24 -0.7 737,648 + Atu3680 0.30 -1.9
Or see this region's nucleotide sequence