Experiment: Ying_Others16 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2832 and Pf6N2E2_2833 are separated by 329 nucleotides Pf6N2E2_2833 and Pf6N2E2_2834 are separated by 1 nucleotides Pf6N2E2_2834 and Pf6N2E2_2835 are separated by 37 nucleotides
Pf6N2E2_2832 - dicarboxylic acid transporter PcaT, at 3,321,298 to 3,322,596
_2832
Pf6N2E2_2833 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 3,322,926 to 3,323,492
_2833
Pf6N2E2_2834 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 3,323,494 to 3,324,198
_2834
Pf6N2E2_2835 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,324,236 to 3,325,438
_2835
Position (kb)
3323
3324
3325 Strain fitness (log2 ratio)
-2
-1
0
1 at 3322.545 kb on + strand at 3322.545 kb on + strand at 3322.546 kb on - strand at 3322.546 kb on - strand at 3322.546 kb on - strand at 3322.572 kb on - strand at 3322.572 kb on - strand at 3322.572 kb on - strand at 3322.602 kb on + strand at 3322.640 kb on - strand at 3322.640 kb on - strand at 3322.640 kb on - strand at 3322.686 kb on + strand at 3322.730 kb on + strand at 3322.730 kb on + strand at 3322.730 kb on + strand at 3322.730 kb on + strand at 3322.731 kb on - strand at 3322.731 kb on - strand at 3322.731 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.747 kb on - strand at 3322.754 kb on + strand at 3322.754 kb on + strand at 3322.754 kb on + strand at 3322.755 kb on - strand at 3322.853 kb on - strand at 3322.985 kb on - strand, within Pf6N2E2_2833 at 3323.086 kb on + strand, within Pf6N2E2_2833 at 3323.199 kb on - strand, within Pf6N2E2_2833 at 3323.199 kb on - strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.293 kb on + strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.294 kb on - strand, within Pf6N2E2_2833 at 3323.449 kb on + strand at 3323.449 kb on + strand at 3323.450 kb on - strand at 3323.450 kb on - strand at 3323.453 kb on - strand at 3323.453 kb on - strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.495 kb on + strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.496 kb on - strand at 3323.558 kb on + strand at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.672 kb on + strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.673 kb on - strand, within Pf6N2E2_2834 at 3323.885 kb on + strand, within Pf6N2E2_2834 at 3323.885 kb on + strand, within Pf6N2E2_2834 at 3323.886 kb on - strand, within Pf6N2E2_2834 at 3324.146 kb on + strand at 3324.146 kb on + strand at 3324.218 kb on + strand at 3324.218 kb on + strand at 3324.219 kb on - strand at 3324.219 kb on - strand at 3324.221 kb on + strand at 3324.221 kb on + strand at 3324.221 kb on + strand at 3324.222 kb on - strand at 3324.224 kb on + strand at 3324.224 kb on + strand at 3324.225 kb on - strand at 3324.225 kb on - strand at 3324.225 kb on - strand at 3324.919 kb on - strand, within Pf6N2E2_2835 at 3324.919 kb on - strand, within Pf6N2E2_2835 at 3324.935 kb on - strand, within Pf6N2E2_2835 at 3324.966 kb on + strand, within Pf6N2E2_2835
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 2 remove 3,322,545 + +1.0 3,322,545 + -0.8 3,322,546 - +0.0 3,322,546 - +1.0 3,322,546 - +1.0 3,322,572 - +1.0 3,322,572 - +1.6 3,322,572 - +0.0 3,322,602 + +1.0 3,322,640 - -0.6 3,322,640 - +1.0 3,322,640 - +1.0 3,322,686 + +1.0 3,322,730 + +1.0 3,322,730 + +0.0 3,322,730 + -0.6 3,322,730 + -0.3 3,322,731 - +1.0 3,322,731 - +0.0 3,322,731 - +1.0 3,322,747 - -2.3 3,322,747 - -0.6 3,322,747 - +1.0 3,322,747 - -0.1 3,322,747 - -0.3 3,322,754 + -0.6 3,322,754 + +0.2 3,322,754 + +0.0 3,322,755 - -0.8 3,322,853 - -0.2 3,322,985 - Pf6N2E2_2833 0.10 -1.0 3,323,086 + Pf6N2E2_2833 0.28 +1.0 3,323,199 - Pf6N2E2_2833 0.48 -0.6 3,323,199 - Pf6N2E2_2833 0.48 -1.0 3,323,293 + Pf6N2E2_2833 0.65 +0.0 3,323,293 + Pf6N2E2_2833 0.65 +0.0 3,323,293 + Pf6N2E2_2833 0.65 +1.0 3,323,294 - Pf6N2E2_2833 0.65 +0.0 3,323,294 - Pf6N2E2_2833 0.65 +1.0 3,323,294 - Pf6N2E2_2833 0.65 -1.6 3,323,294 - Pf6N2E2_2833 0.65 +1.0 3,323,449 + -1.0 3,323,449 + -1.0 3,323,450 - +1.0 3,323,450 - +1.0 3,323,453 - -0.9 3,323,453 - +1.0 3,323,495 + -1.2 3,323,495 + +0.0 3,323,495 + -1.0 3,323,495 + +1.0 3,323,495 + +1.0 3,323,496 - +0.0 3,323,496 - +1.0 3,323,496 - -1.0 3,323,496 - +1.0 3,323,496 - +1.0 3,323,496 - +1.0 3,323,496 - -1.0 3,323,558 + +1.0 3,323,672 + Pf6N2E2_2834 0.25 -1.7 3,323,672 + Pf6N2E2_2834 0.25 -1.6 3,323,672 + Pf6N2E2_2834 0.25 -2.5 3,323,673 - Pf6N2E2_2834 0.25 +1.0 3,323,673 - Pf6N2E2_2834 0.25 +0.0 3,323,673 - Pf6N2E2_2834 0.25 -0.9 3,323,673 - Pf6N2E2_2834 0.25 -1.5 3,323,673 - Pf6N2E2_2834 0.25 -1.6 3,323,885 + Pf6N2E2_2834 0.55 +1.0 3,323,885 + Pf6N2E2_2834 0.55 +1.0 3,323,886 - Pf6N2E2_2834 0.56 +0.0 3,324,146 + -1.0 3,324,146 + -0.4 3,324,218 + +0.0 3,324,218 + +1.0 3,324,219 - +1.0 3,324,219 - -1.2 3,324,221 + +1.0 3,324,221 + +0.0 3,324,221 + +0.3 3,324,222 - +1.0 3,324,224 + +0.0 3,324,224 + -1.0 3,324,225 - +1.0 3,324,225 - +0.0 3,324,225 - +1.0 3,324,919 - Pf6N2E2_2835 0.57 +1.0 3,324,919 - Pf6N2E2_2835 0.57 -0.8 3,324,935 - Pf6N2E2_2835 0.58 +1.0 3,324,966 + Pf6N2E2_2835 0.61 +1.0
Or see this region's nucleotide sequence