Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1525 and Pf6N2E2_1526 are separated by 100 nucleotides Pf6N2E2_1526 and Pf6N2E2_1527 are separated by 257 nucleotides
Pf6N2E2_1525 - Universal stress protein family 5, at 1,782,830 to 1,783,741
_1525
Pf6N2E2_1526 - MgtC family, at 1,783,842 to 1,785,092
_1526
Pf6N2E2_1527 - Universal stress protein family 5, at 1,785,350 to 1,786,273
_1527
Position (kb)
1783
1784
1785
1786 Strain fitness (log2 ratio)
-2
-1
0
1 at 1783.087 kb on - strand, within Pf6N2E2_1525 at 1783.135 kb on - strand at 1783.434 kb on + strand, within Pf6N2E2_1525 at 1783.435 kb on - strand, within Pf6N2E2_1525 at 1783.435 kb on - strand, within Pf6N2E2_1525 at 1783.435 kb on - strand, within Pf6N2E2_1525 at 1783.552 kb on - strand, within Pf6N2E2_1525 at 1783.633 kb on + strand, within Pf6N2E2_1525 at 1783.633 kb on + strand, within Pf6N2E2_1525 at 1783.634 kb on - strand, within Pf6N2E2_1525 at 1783.722 kb on - strand at 1783.722 kb on - strand at 1783.772 kb on + strand at 1783.773 kb on - strand at 1783.911 kb on + strand at 1783.915 kb on + strand at 1783.915 kb on + strand at 1784.266 kb on + strand, within Pf6N2E2_1526 at 1784.267 kb on - strand, within Pf6N2E2_1526 at 1784.267 kb on - strand, within Pf6N2E2_1526 at 1784.275 kb on - strand, within Pf6N2E2_1526 at 1784.275 kb on - strand, within Pf6N2E2_1526 at 1784.503 kb on + strand, within Pf6N2E2_1526 at 1784.602 kb on + strand, within Pf6N2E2_1526 at 1784.603 kb on - strand, within Pf6N2E2_1526 at 1784.603 kb on - strand, within Pf6N2E2_1526 at 1784.809 kb on - strand, within Pf6N2E2_1526 at 1784.972 kb on + strand at 1784.972 kb on + strand at 1784.973 kb on - strand at 1785.258 kb on - strand at 1785.258 kb on - strand at 1785.355 kb on + strand at 1785.355 kb on + strand at 1785.457 kb on + strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.458 kb on - strand, within Pf6N2E2_1527 at 1785.473 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.932 kb on + strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.933 kb on - strand, within Pf6N2E2_1527 at 1785.968 kb on + strand, within Pf6N2E2_1527 at 1786.022 kb on + strand, within Pf6N2E2_1527 at 1786.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 1,783,087 - Pf6N2E2_1525 0.28 -0.6 1,783,135 - +0.8 1,783,434 + Pf6N2E2_1525 0.66 +0.8 1,783,435 - Pf6N2E2_1525 0.66 +0.5 1,783,435 - Pf6N2E2_1525 0.66 +0.2 1,783,435 - Pf6N2E2_1525 0.66 +0.8 1,783,552 - Pf6N2E2_1525 0.79 -0.4 1,783,633 + Pf6N2E2_1525 0.88 -0.2 1,783,633 + Pf6N2E2_1525 0.88 +0.8 1,783,634 - Pf6N2E2_1525 0.88 +0.8 1,783,722 - -0.2 1,783,722 - -2.3 1,783,772 + +0.8 1,783,773 - +0.8 1,783,911 + +0.8 1,783,915 + +0.8 1,783,915 + +0.8 1,784,266 + Pf6N2E2_1526 0.34 -0.2 1,784,267 - Pf6N2E2_1526 0.34 -0.2 1,784,267 - Pf6N2E2_1526 0.34 +0.8 1,784,275 - Pf6N2E2_1526 0.35 -0.2 1,784,275 - Pf6N2E2_1526 0.35 +0.8 1,784,503 + Pf6N2E2_1526 0.53 +0.8 1,784,602 + Pf6N2E2_1526 0.61 -1.2 1,784,603 - Pf6N2E2_1526 0.61 -1.3 1,784,603 - Pf6N2E2_1526 0.61 +0.8 1,784,809 - Pf6N2E2_1526 0.77 -1.5 1,784,972 + +0.8 1,784,972 + -1.2 1,784,973 - -0.6 1,785,258 - -0.8 1,785,258 - +0.8 1,785,355 + -0.2 1,785,355 + +0.8 1,785,457 + Pf6N2E2_1527 0.12 -0.2 1,785,458 - Pf6N2E2_1527 0.12 +0.8 1,785,458 - Pf6N2E2_1527 0.12 +0.6 1,785,458 - Pf6N2E2_1527 0.12 -1.5 1,785,458 - Pf6N2E2_1527 0.12 +0.8 1,785,473 + Pf6N2E2_1527 0.13 -0.2 1,785,932 + Pf6N2E2_1527 0.63 +0.8 1,785,932 + Pf6N2E2_1527 0.63 -0.1 1,785,932 + Pf6N2E2_1527 0.63 -0.2 1,785,932 + Pf6N2E2_1527 0.63 -0.7 1,785,932 + Pf6N2E2_1527 0.63 +0.8 1,785,932 + Pf6N2E2_1527 0.63 -0.2 1,785,932 + Pf6N2E2_1527 0.63 +0.8 1,785,933 - Pf6N2E2_1527 0.63 -0.2 1,785,933 - Pf6N2E2_1527 0.63 -0.2 1,785,933 - Pf6N2E2_1527 0.63 -0.2 1,785,933 - Pf6N2E2_1527 0.63 +0.8 1,785,933 - Pf6N2E2_1527 0.63 -0.4 1,785,933 - Pf6N2E2_1527 0.63 +0.8 1,785,933 - Pf6N2E2_1527 0.63 +0.8 1,785,968 + Pf6N2E2_1527 0.67 +0.8 1,786,022 + Pf6N2E2_1527 0.73 +0.8 1,786,023 - -0.2
Or see this region's nucleotide sequence