Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_115 and Pf6N2E2_116 are separated by 12 nucleotides Pf6N2E2_116 and Pf6N2E2_117 overlap by 8 nucleotides Pf6N2E2_117 and Pf6N2E2_118 overlap by 4 nucleotides
Pf6N2E2_115 - FIG00958408: hypothetical protein, at 161,631 to 162,635
_115
Pf6N2E2_116 - Glycosyltransferase, at 162,648 to 163,784
_116
Pf6N2E2_117 - Glycosyl transferase, group 2 family protein, at 163,777 to 164,736
_117
Pf6N2E2_118 - FIG00961753: hypothetical protein, at 164,733 to 166,103
_118
Position (kb) 162
163
164 Strain fitness (log2 ratio) -2
-1
0
1 at 161.741 kb on - strand, within Pf6N2E2_115 at 161.828 kb on - strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 162.085 kb on + strand, within Pf6N2E2_115 at 162.086 kb on - strand, within Pf6N2E2_115 at 162.086 kb on - strand at 162.278 kb on + strand, within Pf6N2E2_115 at 162.812 kb on - strand, within Pf6N2E2_116 at 162.844 kb on + strand, within Pf6N2E2_116 at 162.949 kb on + strand, within Pf6N2E2_116 at 163.091 kb on - strand, within Pf6N2E2_116 at 163.091 kb on - strand, within Pf6N2E2_116 at 163.256 kb on - strand, within Pf6N2E2_116 at 163.256 kb on - strand, within Pf6N2E2_116 at 163.579 kb on + strand, within Pf6N2E2_116 at 163.735 kb on + strand at 163.741 kb on + strand at 163.742 kb on - strand at 163.768 kb on + strand at 163.769 kb on - strand at 164.705 kb on + strand at 164.705 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 161,741 - Pf6N2E2_115 0.11 -1.1 161,828 - Pf6N2E2_115 0.20 -2.4 161,833 + Pf6N2E2_115 0.20 +0.2 161,833 + Pf6N2E2_115 0.20 -0.1 161,833 + Pf6N2E2_115 0.20 -0.1 161,833 + Pf6N2E2_115 0.20 +0.9 161,834 - Pf6N2E2_115 0.20 -0.7 161,834 - Pf6N2E2_115 0.20 -1.8 161,834 - Pf6N2E2_115 0.20 -0.1 161,834 - Pf6N2E2_115 0.20 -1.1 161,834 - Pf6N2E2_115 0.20 +0.9 161,834 - Pf6N2E2_115 0.20 +0.9 161,834 - Pf6N2E2_115 0.20 -0.8 161,834 - Pf6N2E2_115 0.20 -0.1 161,834 - Pf6N2E2_115 0.20 +0.2 162,085 + Pf6N2E2_115 0.45 -0.8 162,086 - Pf6N2E2_115 0.45 +0.1 162,086 - +0.9 162,278 + Pf6N2E2_115 0.64 +0.9 162,812 - Pf6N2E2_116 0.14 +0.9 162,844 + Pf6N2E2_116 0.17 -0.1 162,949 + Pf6N2E2_116 0.26 +0.9 163,091 - Pf6N2E2_116 0.39 +0.9 163,091 - Pf6N2E2_116 0.39 -1.1 163,256 - Pf6N2E2_116 0.53 -0.8 163,256 - Pf6N2E2_116 0.53 -0.1 163,579 + Pf6N2E2_116 0.82 -0.1 163,735 + -0.7 163,741 + -1.0 163,742 - +0.9 163,768 + -0.9 163,769 - +0.9 164,705 + +1.3 164,705 + -0.0
Or see this region's nucleotide sequence