Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_279 and Pf6N2E2_280 overlap by 24 nucleotides Pf6N2E2_280 and Pf6N2E2_281 are separated by 323 nucleotides Pf6N2E2_281 and Pf6N2E2_282 are separated by 170 nucleotides Pf6N2E2_282 and Pf6N2E2_283 overlap by 8 nucleotides
Pf6N2E2_279 - Isocitrate lyase (EC 4.1.3.1), at 345,874 to 347,199
_279
Pf6N2E2_280 - hypothetical protein, at 347,176 to 347,331
_280
Pf6N2E2_281 - Type II and III secretion system family protein, at 347,655 to 348,416
_281
Pf6N2E2_282 - GNAT family acetyltransferase YjcF, at 348,587 to 349,012
_282
Pf6N2E2_283 - FIG002776: hypothetical protein, at 349,005 to 350,171
_283
Position (kb) 347
348
349 Strain fitness (log2 ratio) -2
-1
0
1 at 346.670 kb on + strand, within Pf6N2E2_279 at 346.728 kb on - strand, within Pf6N2E2_279 at 346.736 kb on + strand, within Pf6N2E2_279 at 346.737 kb on - strand, within Pf6N2E2_279 at 346.737 kb on - strand, within Pf6N2E2_279 at 346.737 kb on - strand, within Pf6N2E2_279 at 346.841 kb on + strand, within Pf6N2E2_279 at 346.910 kb on + strand, within Pf6N2E2_279 at 346.910 kb on + strand, within Pf6N2E2_279 at 346.910 kb on + strand, within Pf6N2E2_279 at 346.911 kb on - strand, within Pf6N2E2_279 at 346.911 kb on - strand, within Pf6N2E2_279 at 346.911 kb on - strand, within Pf6N2E2_279 at 347.205 kb on + strand, within Pf6N2E2_280 at 347.205 kb on + strand, within Pf6N2E2_280 at 347.219 kb on + strand, within Pf6N2E2_280 at 347.220 kb on - strand, within Pf6N2E2_280 at 347.266 kb on - strand, within Pf6N2E2_280 at 347.329 kb on + strand at 347.330 kb on - strand at 347.371 kb on + strand at 347.399 kb on + strand at 347.399 kb on + strand at 347.399 kb on + strand at 347.400 kb on - strand at 347.400 kb on - strand at 347.400 kb on - strand at 347.400 kb on - strand at 347.403 kb on + strand at 347.403 kb on + strand at 347.403 kb on + strand at 347.403 kb on + strand at 347.404 kb on - strand at 347.611 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.714 kb on - strand at 347.826 kb on + strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand at 347.911 kb on + strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 348.165 kb on - strand, within Pf6N2E2_281 at 348.446 kb on + strand at 348.487 kb on + strand at 348.487 kb on + strand at 348.487 kb on + strand at 348.488 kb on - strand at 348.488 kb on - strand at 348.488 kb on - strand at 348.672 kb on + strand, within Pf6N2E2_282 at 348.672 kb on + strand, within Pf6N2E2_282 at 349.006 kb on + strand at 349.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 346,670 + Pf6N2E2_279 0.60 +1.0 346,728 - Pf6N2E2_279 0.64 +1.0 346,736 + Pf6N2E2_279 0.65 +1.0 346,737 - Pf6N2E2_279 0.65 +1.0 346,737 - Pf6N2E2_279 0.65 +1.0 346,737 - Pf6N2E2_279 0.65 -0.6 346,841 + Pf6N2E2_279 0.73 -1.4 346,910 + Pf6N2E2_279 0.78 -1.0 346,910 + Pf6N2E2_279 0.78 +1.0 346,910 + Pf6N2E2_279 0.78 -0.0 346,911 - Pf6N2E2_279 0.78 +1.0 346,911 - Pf6N2E2_279 0.78 +1.0 346,911 - Pf6N2E2_279 0.78 -0.0 347,205 + Pf6N2E2_280 0.19 +1.0 347,205 + Pf6N2E2_280 0.19 -0.6 347,219 + Pf6N2E2_280 0.28 +1.0 347,220 - Pf6N2E2_280 0.28 -1.0 347,266 - Pf6N2E2_280 0.58 +1.0 347,329 + +1.0 347,330 - +1.0 347,371 + +1.0 347,399 + -0.6 347,399 + +1.0 347,399 + +1.0 347,400 - +1.0 347,400 - -0.6 347,400 - -1.6 347,400 - -1.4 347,403 + +1.0 347,403 + +1.0 347,403 + +0.5 347,403 + +1.0 347,404 - +1.0 347,611 + +1.0 347,713 + -0.0 347,713 + -2.5 347,713 + -0.0 347,714 - -0.0 347,826 + Pf6N2E2_281 0.22 +1.0 347,827 - Pf6N2E2_281 0.23 -0.0 347,827 - Pf6N2E2_281 0.23 +1.0 347,827 - +1.0 347,911 + Pf6N2E2_281 0.34 +1.0 347,912 - Pf6N2E2_281 0.34 -1.2 347,912 - Pf6N2E2_281 0.34 -0.9 347,962 + Pf6N2E2_281 0.40 +1.0 347,962 + Pf6N2E2_281 0.40 -0.0 347,962 + Pf6N2E2_281 0.40 +1.0 347,962 + Pf6N2E2_281 0.40 +1.0 347,963 - Pf6N2E2_281 0.40 -0.0 347,963 - Pf6N2E2_281 0.40 -2.4 347,963 - Pf6N2E2_281 0.40 +1.0 348,165 - Pf6N2E2_281 0.67 -0.6 348,446 + +1.0 348,487 + -0.2 348,487 + -0.0 348,487 + -0.6 348,488 - -1.0 348,488 - +1.0 348,488 - +1.0 348,672 + Pf6N2E2_282 0.20 +1.0 348,672 + Pf6N2E2_282 0.20 +1.0 349,006 + -0.6 349,007 - -0.8
Or see this region's nucleotide sequence