Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_513

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_512 and Pf6N2E2_513 are separated by 194 nucleotidesPf6N2E2_513 and Pf6N2E2_514 overlap by 4 nucleotidesPf6N2E2_514 and Pf6N2E2_515 are separated by 30 nucleotides Pf6N2E2_512 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data), at 580,158 to 582,095 _512 Pf6N2E2_513 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data), at 582,290 to 583,183 _513 Pf6N2E2_514 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data), at 583,180 to 583,989 _514 Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (from data), at 584,020 to 585,522 _515 Position (kb) 582 583 584Strain fitness (log2 ratio) -2 -1 0 1 2at 581.293 kb on + strand, within Pf6N2E2_512at 581.500 kb on + strand, within Pf6N2E2_512at 581.501 kb on - strand, within Pf6N2E2_512at 581.658 kb on + strand, within Pf6N2E2_512at 581.658 kb on + strand, within Pf6N2E2_512at 581.658 kb on + strand, within Pf6N2E2_512at 581.658 kb on + strand, within Pf6N2E2_512at 581.659 kb on - strand, within Pf6N2E2_512at 581.659 kb on - strand, within Pf6N2E2_512at 581.659 kb on - strand, within Pf6N2E2_512at 581.746 kb on - strand, within Pf6N2E2_512at 581.767 kb on - strand, within Pf6N2E2_512at 581.778 kb on - strand, within Pf6N2E2_512at 582.099 kb on + strandat 582.100 kb on - strandat 582.100 kb on - strandat 582.263 kb on + strandat 582.263 kb on + strandat 582.264 kb on - strandat 582.264 kb on - strandat 582.264 kb on - strandat 582.275 kb on + strandat 582.275 kb on + strandat 582.275 kb on + strandat 582.275 kb on + strandat 582.275 kb on + strandat 582.275 kb on + strandat 582.275 kb on + strandat 582.276 kb on - strandat 582.276 kb on - strandat 582.276 kb on - strandat 582.276 kb on - strandat 582.276 kb on - strandat 582.276 kb on - strandat 582.473 kb on + strand, within Pf6N2E2_513at 582.501 kb on - strand, within Pf6N2E2_513at 582.735 kb on + strand, within Pf6N2E2_513at 582.747 kb on - strand, within Pf6N2E2_513at 582.747 kb on - strand, within Pf6N2E2_513at 582.767 kb on + strand, within Pf6N2E2_513at 582.768 kb on - strand, within Pf6N2E2_513at 582.851 kb on + strand, within Pf6N2E2_513at 582.851 kb on + strand, within Pf6N2E2_513at 582.851 kb on + strand, within Pf6N2E2_513at 582.851 kb on + strand, within Pf6N2E2_513at 582.851 kb on + strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 582.852 kb on - strand, within Pf6N2E2_513at 583.064 kb on + strand, within Pf6N2E2_513at 583.107 kb on + strandat 583.108 kb on - strandat 583.118 kb on + strandat 583.118 kb on + strandat 583.118 kb on + strandat 583.118 kb on + strandat 583.119 kb on - strandat 583.119 kb on - strandat 583.140 kb on - strandat 583.249 kb on + strandat 583.249 kb on + strandat 583.249 kb on + strandat 583.250 kb on - strandat 583.250 kb on - strandat 583.435 kb on + strand, within Pf6N2E2_514at 583.436 kb on - strand, within Pf6N2E2_514at 583.490 kb on + strand, within Pf6N2E2_514at 583.491 kb on - strand, within Pf6N2E2_514at 583.534 kb on + strand, within Pf6N2E2_514at 583.535 kb on - strand, within Pf6N2E2_514at 583.535 kb on - strand, within Pf6N2E2_514at 583.604 kb on - strand, within Pf6N2E2_514at 583.750 kb on + strand, within Pf6N2E2_514at 583.750 kb on + strandat 583.751 kb on - strand, within Pf6N2E2_514at 583.751 kb on - strand, within Pf6N2E2_514at 583.798 kb on + strand, within Pf6N2E2_514at 583.798 kb on + strand, within Pf6N2E2_514at 583.799 kb on - strand, within Pf6N2E2_514at 583.799 kb on - strand, within Pf6N2E2_514at 583.906 kb on + strand, within Pf6N2E2_514at 583.907 kb on - strand, within Pf6N2E2_514at 583.907 kb on - strand, within Pf6N2E2_514at 583.909 kb on + strandat 584.003 kb on + strandat 584.047 kb on + strandat 584.047 kb on + strandat 584.048 kb on - strandat 584.048 kb on - strandat 584.048 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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581,293 + Pf6N2E2_512 0.59 +2.4
581,500 + Pf6N2E2_512 0.69 -1.0
581,501 - Pf6N2E2_512 0.69 +0.9
581,658 + Pf6N2E2_512 0.77 +0.9
581,658 + Pf6N2E2_512 0.77 -0.7
581,658 + Pf6N2E2_512 0.77 -1.5
581,658 + Pf6N2E2_512 0.77 +0.2
581,659 - Pf6N2E2_512 0.77 -1.1
581,659 - Pf6N2E2_512 0.77 -2.3
581,659 - Pf6N2E2_512 0.77 -0.7
581,746 - Pf6N2E2_512 0.82 -0.9
581,767 - Pf6N2E2_512 0.83 -0.1
581,778 - Pf6N2E2_512 0.84 -0.2
582,099 + -0.1
582,100 - -0.1
582,100 - +0.9
582,263 + -0.3
582,263 + -0.1
582,264 - -0.1
582,264 - -0.3
582,264 - -0.7
582,275 + -1.0
582,275 + -1.0
582,275 + +0.9
582,275 + -0.7
582,275 + -1.1
582,275 + +0.9
582,275 + -2.0
582,276 - -0.1
582,276 - -1.4
582,276 - -0.1
582,276 - +0.9
582,276 - +0.9
582,276 - -0.4
582,473 + Pf6N2E2_513 0.20 +0.9
582,501 - Pf6N2E2_513 0.24 +0.9
582,735 + Pf6N2E2_513 0.50 -0.1
582,747 - Pf6N2E2_513 0.51 -1.1
582,747 - Pf6N2E2_513 0.51 -0.1
582,767 + Pf6N2E2_513 0.53 +0.9
582,768 - Pf6N2E2_513 0.53 -0.1
582,851 + Pf6N2E2_513 0.63 -0.1
582,851 + Pf6N2E2_513 0.63 +0.9
582,851 + Pf6N2E2_513 0.63 -0.1
582,851 + Pf6N2E2_513 0.63 -0.1
582,851 + Pf6N2E2_513 0.63 -1.0
582,852 - Pf6N2E2_513 0.63 -0.1
582,852 - Pf6N2E2_513 0.63 -0.1
582,852 - Pf6N2E2_513 0.63 -0.7
582,852 - Pf6N2E2_513 0.63 -0.1
582,852 - Pf6N2E2_513 0.63 +0.9
582,852 - Pf6N2E2_513 0.63 +0.9
582,852 - Pf6N2E2_513 0.63 +1.1
583,064 + Pf6N2E2_513 0.87 -0.1
583,107 + +0.9
583,108 - +0.4
583,118 + +0.9
583,118 + -0.6
583,118 + +0.9
583,118 + +0.9
583,119 - -0.1
583,119 - +0.9
583,140 - -0.7
583,249 + +0.9
583,249 + +0.9
583,249 + -0.1
583,250 - +0.9
583,250 - +0.1
583,435 + Pf6N2E2_514 0.31 -0.1
583,436 - Pf6N2E2_514 0.32 -0.4
583,490 + Pf6N2E2_514 0.38 +0.9
583,491 - Pf6N2E2_514 0.38 +0.9
583,534 + Pf6N2E2_514 0.44 +0.2
583,535 - Pf6N2E2_514 0.44 +0.9
583,535 - Pf6N2E2_514 0.44 +0.9
583,604 - Pf6N2E2_514 0.52 -0.5
583,750 + Pf6N2E2_514 0.70 +0.9
583,750 + +0.9
583,751 - Pf6N2E2_514 0.70 +0.9
583,751 - Pf6N2E2_514 0.70 +0.9
583,798 + Pf6N2E2_514 0.76 +0.9
583,798 + Pf6N2E2_514 0.76 -0.1
583,799 - Pf6N2E2_514 0.76 +0.0
583,799 - Pf6N2E2_514 0.76 -1.0
583,906 + Pf6N2E2_514 0.90 -1.1
583,907 - Pf6N2E2_514 0.90 +0.9
583,907 - Pf6N2E2_514 0.90 -0.3
583,909 + +0.9
584,003 + +0.6
584,047 + +0.9
584,047 + +0.9
584,048 - +0.9
584,048 - -0.1
584,048 - +0.9

Or see this region's nucleotide sequence