Experiment: Ying_OrganicAcid7 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2928 and Pf6N2E2_2929 overlap by 4 nucleotides Pf6N2E2_2929 and Pf6N2E2_2930 overlap by 4 nucleotides Pf6N2E2_2930 and Pf6N2E2_2931 are separated by 84 nucleotides
Pf6N2E2_2928 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,420,506 to 3,421,375
_2928
Pf6N2E2_2929 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,421,372 to 3,422,130
_2929
Pf6N2E2_2930 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 3,422,127 to 3,422,606
_2930
Pf6N2E2_2931 - Transcriptional regulator, LysR family, at 3,422,691 to 3,423,647
_2931
Position (kb)
3422
3423 Strain fitness (log2 ratio)
-2
-1
0
1 at 3421.194 kb on + strand, within Pf6N2E2_2928 at 3421.238 kb on + strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.586 kb on - strand, within Pf6N2E2_2929 at 3421.684 kb on + strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.102 kb on - strand at 3422.102 kb on - strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.109 kb on - strand at 3422.121 kb on - strand at 3422.121 kb on - strand at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.536 kb on - strand, within Pf6N2E2_2930 at 3422.620 kb on + strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.693 kb on - strand at 3422.747 kb on - strand at 3422.747 kb on - strand at 3422.788 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.938 kb on + strand, within Pf6N2E2_2931 at 3422.938 kb on + strand, within Pf6N2E2_2931 at 3422.939 kb on - strand, within Pf6N2E2_2931 at 3422.939 kb on - strand, within Pf6N2E2_2931 at 3422.946 kb on - strand, within Pf6N2E2_2931 at 3422.998 kb on + strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3423.022 kb on + strand, within Pf6N2E2_2931 at 3423.022 kb on + strand, within Pf6N2E2_2931 at 3423.023 kb on - strand, within Pf6N2E2_2931 at 3423.023 kb on - strand, within Pf6N2E2_2931 at 3423.029 kb on + strand, within Pf6N2E2_2931 at 3423.082 kb on + strand, within Pf6N2E2_2931 at 3423.253 kb on + strand, within Pf6N2E2_2931 at 3423.254 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.338 kb on + strand, within Pf6N2E2_2931
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 2 remove 3,421,194 + Pf6N2E2_2928 0.79 +0.4 3,421,238 + Pf6N2E2_2928 0.84 -1.3 3,421,239 - Pf6N2E2_2928 0.84 +1.0 3,421,239 - Pf6N2E2_2928 0.84 +1.0 3,421,239 - Pf6N2E2_2928 0.84 -0.7 3,421,239 - Pf6N2E2_2928 0.84 -0.0 3,421,239 - Pf6N2E2_2928 0.84 -0.9 3,421,239 - Pf6N2E2_2928 0.84 -1.0 3,421,585 + Pf6N2E2_2929 0.28 -1.0 3,421,585 + Pf6N2E2_2929 0.28 -0.0 3,421,585 + Pf6N2E2_2929 0.28 +1.0 3,421,585 + Pf6N2E2_2929 0.28 +1.0 3,421,586 - Pf6N2E2_2929 0.28 +1.0 3,421,684 + Pf6N2E2_2929 0.41 +1.0 3,421,685 - Pf6N2E2_2929 0.41 -0.0 3,421,685 - Pf6N2E2_2929 0.41 -0.7 3,422,101 + -0.0 3,422,101 + +0.2 3,422,101 + -0.6 3,422,101 + +0.2 3,422,102 - -0.0 3,422,102 - -1.6 3,422,108 + +1.0 3,422,108 + -0.0 3,422,108 + -1.0 3,422,109 - -2.5 3,422,121 - +1.0 3,422,121 - -0.0 3,422,513 + Pf6N2E2_2930 0.80 +1.0 3,422,513 + Pf6N2E2_2930 0.80 -1.7 3,422,513 + Pf6N2E2_2930 0.80 +1.0 3,422,513 + Pf6N2E2_2930 0.80 -0.0 3,422,513 + Pf6N2E2_2930 0.80 -1.5 3,422,536 - Pf6N2E2_2930 0.85 -0.0 3,422,620 + +1.0 3,422,621 - +1.0 3,422,621 - +1.0 3,422,621 - -0.6 3,422,621 - -0.0 3,422,693 - -1.8 3,422,747 - -0.6 3,422,747 - +1.0 3,422,788 + Pf6N2E2_2931 0.10 -0.8 3,422,803 + Pf6N2E2_2931 0.12 -0.0 3,422,803 + Pf6N2E2_2931 0.12 -0.0 3,422,803 + Pf6N2E2_2931 0.12 +1.0 3,422,804 - Pf6N2E2_2931 0.12 -1.9 3,422,804 - Pf6N2E2_2931 0.12 -0.0 3,422,804 - Pf6N2E2_2931 0.12 +1.0 3,422,804 - Pf6N2E2_2931 0.12 -0.0 3,422,804 - Pf6N2E2_2931 0.12 -1.2 3,422,938 + Pf6N2E2_2931 0.26 +1.0 3,422,938 + Pf6N2E2_2931 0.26 +1.0 3,422,939 - Pf6N2E2_2931 0.26 -1.5 3,422,939 - Pf6N2E2_2931 0.26 +1.0 3,422,946 - Pf6N2E2_2931 0.27 -0.6 3,422,998 + Pf6N2E2_2931 0.32 -0.0 3,422,999 - Pf6N2E2_2931 0.32 -1.4 3,422,999 - Pf6N2E2_2931 0.32 -0.4 3,422,999 - Pf6N2E2_2931 0.32 -0.6 3,423,022 + Pf6N2E2_2931 0.35 -1.4 3,423,022 + Pf6N2E2_2931 0.35 +1.0 3,423,023 - Pf6N2E2_2931 0.35 -1.0 3,423,023 - Pf6N2E2_2931 0.35 -1.0 3,423,029 + Pf6N2E2_2931 0.35 -0.0 3,423,082 + Pf6N2E2_2931 0.41 +1.0 3,423,253 + Pf6N2E2_2931 0.59 +1.0 3,423,254 - Pf6N2E2_2931 0.59 -0.0 3,423,258 - Pf6N2E2_2931 0.59 -0.6 3,423,258 - Pf6N2E2_2931 0.59 +1.0 3,423,258 - Pf6N2E2_2931 0.59 -0.7 3,423,258 - Pf6N2E2_2931 0.59 -0.6 3,423,338 + Pf6N2E2_2931 0.68 +1.0
Or see this region's nucleotide sequence