Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_830

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_829 and Pf6N2E2_830 are separated by 51 nucleotidesPf6N2E2_830 and Pf6N2E2_831 are separated by 57 nucleotidesPf6N2E2_831 and Pf6N2E2_832 are separated by 48 nucleotides Pf6N2E2_829 - TesB-like acyl-CoA thioesterase 1, at 967,218 to 967,997 _829 Pf6N2E2_830 - Long-chain fatty acid transport protein, at 968,049 to 969,320 _830 Pf6N2E2_831 - Ketoacyl reductase hetN (EC 1.3.1.-), at 969,378 to 970,163 _831 Pf6N2E2_832 - Outer membrane component of tripartite multidrug resistance system, at 970,212 to 971,675 _832 Position (kb) 968 969 970Strain fitness (log2 ratio) -2 -1 0 1at 967.077 kb on - strandat 967.142 kb on + strandat 967.215 kb on + strandat 967.216 kb on - strandat 967.216 kb on - strandat 967.216 kb on - strandat 967.399 kb on + strand, within Pf6N2E2_829at 967.399 kb on + strand, within Pf6N2E2_829at 967.399 kb on + strand, within Pf6N2E2_829at 967.400 kb on - strand, within Pf6N2E2_829at 967.400 kb on - strand, within Pf6N2E2_829at 967.928 kb on + strandat 967.991 kb on + strandat 967.992 kb on - strandat 967.992 kb on - strandat 967.995 kb on + strandat 967.996 kb on - strandat 967.998 kb on + strandat 967.998 kb on + strandat 967.999 kb on - strandat 968.036 kb on + strandat 968.036 kb on + strandat 968.036 kb on + strandat 968.037 kb on - strandat 968.037 kb on - strandat 968.037 kb on - strandat 968.037 kb on - strandat 968.037 kb on - strandat 968.044 kb on + strandat 968.044 kb on + strandat 968.045 kb on - strandat 968.045 kb on - strandat 968.045 kb on - strandat 968.047 kb on + strandat 968.047 kb on + strandat 968.047 kb on + strandat 968.048 kb on - strandat 968.144 kb on + strandat 968.144 kb on + strandat 968.145 kb on - strandat 968.145 kb on - strandat 968.145 kb on - strandat 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.367 kb on + strand, within Pf6N2E2_830at 968.368 kb on - strand, within Pf6N2E2_830at 968.412 kb on + strand, within Pf6N2E2_830at 968.413 kb on - strand, within Pf6N2E2_830at 968.466 kb on + strand, within Pf6N2E2_830at 968.517 kb on + strand, within Pf6N2E2_830at 968.517 kb on + strand, within Pf6N2E2_830at 968.518 kb on - strand, within Pf6N2E2_830at 968.518 kb on - strand, within Pf6N2E2_830at 968.518 kb on - strand, within Pf6N2E2_830at 968.718 kb on + strand, within Pf6N2E2_830at 968.718 kb on + strand, within Pf6N2E2_830at 968.718 kb on + strand, within Pf6N2E2_830at 968.736 kb on + strand, within Pf6N2E2_830at 968.737 kb on - strand, within Pf6N2E2_830at 968.737 kb on - strand, within Pf6N2E2_830at 968.805 kb on + strand, within Pf6N2E2_830at 968.806 kb on - strand, within Pf6N2E2_830at 968.806 kb on - strand, within Pf6N2E2_830at 968.886 kb on + strand, within Pf6N2E2_830at 968.886 kb on + strand, within Pf6N2E2_830at 968.887 kb on - strand, within Pf6N2E2_830at 968.887 kb on - strand, within Pf6N2E2_830at 968.898 kb on - strand, within Pf6N2E2_830at 969.037 kb on - strand, within Pf6N2E2_830at 969.037 kb on - strand, within Pf6N2E2_830at 969.038 kb on + strand, within Pf6N2E2_830at 969.198 kb on + strandat 969.204 kb on + strandat 969.204 kb on + strandat 969.204 kb on + strandat 969.204 kb on + strandat 969.205 kb on - strandat 969.205 kb on - strandat 969.258 kb on + strandat 969.258 kb on + strandat 969.258 kb on + strandat 969.258 kb on + strandat 969.258 kb on + strandat 969.259 kb on - strandat 969.259 kb on - strandat 969.296 kb on + strandat 969.297 kb on - strandat 969.680 kb on - strand, within Pf6N2E2_831at 969.697 kb on + strand, within Pf6N2E2_831at 969.697 kb on + strand, within Pf6N2E2_831at 969.698 kb on - strand, within Pf6N2E2_831at 969.725 kb on - strand, within Pf6N2E2_831at 970.087 kb on + strandat 970.195 kb on + strandat 970.196 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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967,077 - -0.5
967,142 + +0.8
967,215 + -0.5
967,216 - +0.8
967,216 - +0.8
967,216 - +0.8
967,399 + Pf6N2E2_829 0.23 -0.2
967,399 + Pf6N2E2_829 0.23 -1.5
967,399 + Pf6N2E2_829 0.23 -1.8
967,400 - Pf6N2E2_829 0.23 +0.8
967,400 - Pf6N2E2_829 0.23 +0.8
967,928 + +0.8
967,991 + -0.2
967,992 - -0.2
967,992 - +0.8
967,995 + -0.2
967,996 - +0.8
967,998 + +0.8
967,998 + +0.8
967,999 - +0.8
968,036 + -0.8
968,036 + -0.2
968,036 + +0.8
968,037 - -2.5
968,037 - +0.8
968,037 - -0.5
968,037 - -1.8
968,037 - +0.8
968,044 + +0.8
968,044 + +0.8
968,045 - +0.8
968,045 - +0.8
968,045 - -1.7
968,047 + -1.4
968,047 + -0.2
968,047 + +0.8
968,048 - +0.8
968,144 + +0.8
968,144 + +0.8
968,145 - -2.3
968,145 - +0.8
968,145 - -1.6
968,367 + Pf6N2E2_830 0.25 +0.8
968,367 + Pf6N2E2_830 0.25 -0.2
968,367 + Pf6N2E2_830 0.25 +0.8
968,367 + Pf6N2E2_830 0.25 -2.4
968,367 + Pf6N2E2_830 0.25 -1.8
968,367 + Pf6N2E2_830 0.25 +0.8
968,367 + Pf6N2E2_830 0.25 +0.8
968,367 + Pf6N2E2_830 0.25 +0.8
968,368 - Pf6N2E2_830 0.25 -0.8
968,412 + Pf6N2E2_830 0.29 -2.1
968,413 - Pf6N2E2_830 0.29 -2.0
968,466 + Pf6N2E2_830 0.33 +0.8
968,517 + Pf6N2E2_830 0.37 +0.8
968,517 + Pf6N2E2_830 0.37 -0.8
968,518 - Pf6N2E2_830 0.37 -0.2
968,518 - Pf6N2E2_830 0.37 +0.8
968,518 - Pf6N2E2_830 0.37 -2.4
968,718 + Pf6N2E2_830 0.53 +0.8
968,718 + Pf6N2E2_830 0.53 -2.0
968,718 + Pf6N2E2_830 0.53 -1.8
968,736 + Pf6N2E2_830 0.54 +0.8
968,737 - Pf6N2E2_830 0.54 -0.2
968,737 - Pf6N2E2_830 0.54 +0.8
968,805 + Pf6N2E2_830 0.59 -2.4
968,806 - Pf6N2E2_830 0.60 -0.2
968,806 - Pf6N2E2_830 0.60 -1.8
968,886 + Pf6N2E2_830 0.66 +0.8
968,886 + Pf6N2E2_830 0.66 -1.2
968,887 - Pf6N2E2_830 0.66 +0.8
968,887 - Pf6N2E2_830 0.66 -0.2
968,898 - Pf6N2E2_830 0.67 -0.2
969,037 - Pf6N2E2_830 0.78 -1.2
969,037 - Pf6N2E2_830 0.78 -0.2
969,038 + Pf6N2E2_830 0.78 -1.0
969,198 + +0.8
969,204 + -1.9
969,204 + +0.8
969,204 + +0.8
969,204 + -0.8
969,205 - -1.2
969,205 - +0.8
969,258 + +0.8
969,258 + -1.8
969,258 + -2.2
969,258 + -0.8
969,258 + +0.8
969,259 - -0.2
969,259 - -0.2
969,296 + -1.8
969,297 - -1.8
969,680 - Pf6N2E2_831 0.38 +0.8
969,697 + Pf6N2E2_831 0.41 -1.5
969,697 + Pf6N2E2_831 0.41 -0.8
969,698 - Pf6N2E2_831 0.41 +0.8
969,725 - Pf6N2E2_831 0.44 -2.0
970,087 + +0.8
970,195 + -0.2
970,196 - +0.8

Or see this region's nucleotide sequence