Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_479 and Pf6N2E2_480 are separated by 0 nucleotides Pf6N2E2_480 and Pf6N2E2_481 are separated by 1 nucleotides
Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) <small>(from data)</small>, at 544,889 to 546,160
_479
Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) <small>(from data)</small>, at 546,161 to 547,219
_480
Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) <small>(from data)</small>, at 547,221 to 548,456
_481
Position (kb) 546
547
548 Strain fitness (log2 ratio) -2
-1
0
1 at 545.497 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.643 kb on + strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 546.053 kb on + strand at 546.053 kb on + strand at 546.146 kb on - strand at 546.216 kb on + strand at 546.216 kb on + strand at 546.217 kb on - strand at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand at 546.561 kb on + strand, within Pf6N2E2_480 at 546.561 kb on + strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.565 kb on - strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.721 kb on - strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.913 kb on - strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 547.032 kb on + strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.077 kb on + strand, within Pf6N2E2_480 at 547.078 kb on - strand at 547.198 kb on + strand at 547.199 kb on - strand at 547.199 kb on - strand at 547.274 kb on - strand at 547.274 kb on - strand at 547.427 kb on + strand, within Pf6N2E2_481 at 547.427 kb on + strand, within Pf6N2E2_481 at 547.427 kb on + strand, within Pf6N2E2_481 at 547.427 kb on + strand, within Pf6N2E2_481 at 547.428 kb on - strand, within Pf6N2E2_481 at 547.428 kb on - strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.945 kb on + strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.946 kb on - strand, within Pf6N2E2_481 at 547.957 kb on - strand, within Pf6N2E2_481 at 548.137 kb on + strand, within Pf6N2E2_481 at 548.138 kb on - strand, within Pf6N2E2_481 at 548.185 kb on + strand, within Pf6N2E2_481 at 548.185 kb on + strand at 548.186 kb on - strand, within Pf6N2E2_481
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 545,497 - Pf6N2E2_479 0.48 -2.2 545,599 - Pf6N2E2_479 0.56 +0.9 545,599 - Pf6N2E2_479 0.56 +0.9 545,643 + Pf6N2E2_479 0.59 -1.9 545,644 - Pf6N2E2_479 0.59 +0.9 545,644 - Pf6N2E2_479 0.59 +0.9 546,053 + +0.9 546,053 + +0.9 546,146 - -1.5 546,216 + -1.8 546,216 + +0.9 546,217 - -2.0 546,483 + Pf6N2E2_480 0.30 -0.1 546,483 + Pf6N2E2_480 0.30 -0.1 546,483 + Pf6N2E2_480 0.30 +0.9 546,483 + Pf6N2E2_480 0.30 +0.9 546,483 + Pf6N2E2_480 0.30 -1.8 546,484 - Pf6N2E2_480 0.31 +0.9 546,484 - Pf6N2E2_480 0.31 -0.1 546,484 - Pf6N2E2_480 0.31 -1.9 546,484 - Pf6N2E2_480 0.31 -1.8 546,484 - Pf6N2E2_480 0.31 +0.9 546,484 - Pf6N2E2_480 0.31 +0.9 546,484 - Pf6N2E2_480 0.31 +0.9 546,484 - Pf6N2E2_480 0.31 +0.1 546,484 - Pf6N2E2_480 0.31 +0.9 546,484 - Pf6N2E2_480 0.31 -1.1 546,484 - Pf6N2E2_480 0.31 -0.1 546,498 + Pf6N2E2_480 0.32 +0.9 546,498 + Pf6N2E2_480 0.32 -0.1 546,498 + Pf6N2E2_480 0.32 -0.7 546,499 - Pf6N2E2_480 0.32 -0.1 546,499 - Pf6N2E2_480 0.32 +0.9 546,499 - +0.9 546,561 + Pf6N2E2_480 0.38 +0.9 546,561 + Pf6N2E2_480 0.38 -0.7 546,562 - Pf6N2E2_480 0.38 -0.1 546,562 - Pf6N2E2_480 0.38 -1.1 546,562 - Pf6N2E2_480 0.38 -0.7 546,565 - Pf6N2E2_480 0.38 -0.4 546,645 + Pf6N2E2_480 0.46 +0.9 546,645 + Pf6N2E2_480 0.46 -0.1 546,645 + Pf6N2E2_480 0.46 +0.9 546,646 - Pf6N2E2_480 0.46 +0.9 546,646 - Pf6N2E2_480 0.46 +0.9 546,721 - Pf6N2E2_480 0.53 +0.9 546,804 + Pf6N2E2_480 0.61 +0.9 546,804 + Pf6N2E2_480 0.61 -0.1 546,804 + Pf6N2E2_480 0.61 -0.1 546,804 + Pf6N2E2_480 0.61 +0.9 546,804 + Pf6N2E2_480 0.61 +0.9 546,805 - Pf6N2E2_480 0.61 +0.9 546,805 - Pf6N2E2_480 0.61 +0.9 546,913 - Pf6N2E2_480 0.71 +0.9 546,918 + Pf6N2E2_480 0.71 +0.9 546,918 + Pf6N2E2_480 0.71 +0.9 546,918 + Pf6N2E2_480 0.71 +0.9 546,918 + Pf6N2E2_480 0.71 -0.1 546,919 - Pf6N2E2_480 0.72 -0.7 546,919 - Pf6N2E2_480 0.72 -0.1 546,919 - Pf6N2E2_480 0.72 +0.9 546,919 - Pf6N2E2_480 0.72 +0.9 547,032 + Pf6N2E2_480 0.82 +0.9 547,033 - Pf6N2E2_480 0.82 -0.1 547,033 - Pf6N2E2_480 0.82 +0.9 547,033 - Pf6N2E2_480 0.82 +0.3 547,077 + Pf6N2E2_480 0.86 -0.1 547,078 - +0.9 547,198 + +0.3 547,199 - -0.1 547,199 - -0.1 547,274 - +0.9 547,274 - -0.1 547,427 + Pf6N2E2_481 0.17 -1.1 547,427 + Pf6N2E2_481 0.17 +0.9 547,427 + Pf6N2E2_481 0.17 -0.1 547,427 + Pf6N2E2_481 0.17 -2.2 547,428 - Pf6N2E2_481 0.17 +0.9 547,428 - Pf6N2E2_481 0.17 +0.9 547,945 + Pf6N2E2_481 0.59 +0.9 547,945 + Pf6N2E2_481 0.59 -0.1 547,945 + Pf6N2E2_481 0.59 +0.9 547,945 + Pf6N2E2_481 0.59 +0.9 547,946 - Pf6N2E2_481 0.59 +0.9 547,946 - Pf6N2E2_481 0.59 -1.3 547,946 - Pf6N2E2_481 0.59 +0.9 547,946 - Pf6N2E2_481 0.59 -2.0 547,957 - Pf6N2E2_481 0.60 +0.9 548,137 + Pf6N2E2_481 0.74 +0.9 548,138 - Pf6N2E2_481 0.74 -0.6 548,185 + Pf6N2E2_481 0.78 +0.9 548,185 + +0.9 548,186 - Pf6N2E2_481 0.78 +0.9
Or see this region's nucleotide sequence