Experiment: JP1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14865 and PS417_14870 are separated by 90 nucleotides PS417_14870 and PS417_14875 overlap by 4 nucleotides PS417_14875 and PS417_14880 are separated by 133 nucleotides PS417_14880 and PS417_14885 are separated by 43 nucleotides
PS417_14865: PS417_14865 - GntR family transcriptional regulator, at 3,247,240 to 3,248,565
_14865
PS417_14870: PS417_14870 - transporter, at 3,248,656 to 3,249,546
_14870
PS417_14875: PS417_14875 - polyisoprenoid-binding protein, at 3,249,543 to 3,250,097
_14875
PS417_14880: PS417_14880 - thioesterase, at 3,250,231 to 3,250,725
_14880
PS417_14885: PS417_14885 - dienelactone hydrolase, at 3,250,769 to 3,251,656
_14885
Position (kb)
3249
3250
3251 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3248.573 kb on + strand at 3248.573 kb on + strand at 3248.573 kb on + strand at 3248.574 kb on - strand at 3248.574 kb on - strand at 3248.574 kb on - strand at 3248.599 kb on + strand at 3248.665 kb on - strand at 3248.976 kb on - strand, within PS417_14870 at 3249.068 kb on + strand, within PS417_14870 at 3249.072 kb on + strand, within PS417_14870 at 3249.072 kb on + strand, within PS417_14870 at 3249.072 kb on + strand, within PS417_14870 at 3249.328 kb on + strand, within PS417_14870 at 3249.794 kb on - strand, within PS417_14875 at 3249.968 kb on - strand, within PS417_14875 at 3250.034 kb on - strand, within PS417_14875 at 3250.060 kb on + strand at 3250.061 kb on - strand at 3250.099 kb on - strand at 3250.099 kb on - strand at 3250.099 kb on - strand at 3250.193 kb on + strand at 3250.344 kb on - strand, within PS417_14880 at 3250.932 kb on - strand, within PS417_14885 at 3250.932 kb on - strand, within PS417_14885 at 3250.982 kb on + strand, within PS417_14885 at 3250.991 kb on + strand, within PS417_14885 at 3250.992 kb on - strand, within PS417_14885 at 3251.008 kb on + strand, within PS417_14885 at 3251.008 kb on + strand, within PS417_14885
Per-strain Table
Position Strand Gene LocusTag Fraction JP1 MOI 1 remove 3,248,573 + -1.1 3,248,573 + +0.7 3,248,573 + -1.2 3,248,574 - +1.3 3,248,574 - -1.5 3,248,574 - +0.1 3,248,599 + -0.1 3,248,665 - +0.4 3,248,976 - PS417_14870 0.36 +0.4 3,249,068 + PS417_14870 0.46 +0.9 3,249,072 + PS417_14870 0.47 +1.1 3,249,072 + PS417_14870 0.47 +1.4 3,249,072 + PS417_14870 0.47 -1.6 3,249,328 + PS417_14870 0.75 -0.7 3,249,794 - PS417_14875 0.45 -0.2 3,249,968 - PS417_14875 0.77 +0.9 3,250,034 - PS417_14875 0.88 -0.8 3,250,060 + +0.5 3,250,061 - +2.9 3,250,099 - -0.6 3,250,099 - -3.2 3,250,099 - -0.3 3,250,193 + +0.7 3,250,344 - PS417_14880 0.23 -0.6 3,250,932 - PS417_14885 0.18 -1.0 3,250,932 - PS417_14885 0.18 +1.1 3,250,982 + PS417_14885 0.24 -1.5 3,250,991 + PS417_14885 0.25 +0.6 3,250,992 - PS417_14885 0.25 +0.2 3,251,008 + PS417_14885 0.27 +1.3 3,251,008 + PS417_14885 0.27 +0.8
Or see this region's nucleotide sequence