Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4396

Experiment: nitrogen source nitrite 1mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4394 and Pf6N2E2_4395 are separated by 182 nucleotidesPf6N2E2_4395 and Pf6N2E2_4396 are separated by 136 nucleotidesPf6N2E2_4396 and Pf6N2E2_4397 are separated by 56 nucleotidesPf6N2E2_4397 and Pf6N2E2_4398 are separated by 57 nucleotides Pf6N2E2_4394 - Putrescine utilization regulator, at 5,122,456 to 5,123,004 _4394 Pf6N2E2_4395 - Cytochrome c5, at 5,123,187 to 5,123,600 _4395 Pf6N2E2_4396 - Acetyl-CoA hydrolase, at 5,123,737 to 5,125,659 _4396 Pf6N2E2_4397 - Xanthine phosphoribosyltransferase (EC 2.4.2.22), at 5,125,716 to 5,126,288 _4397 Pf6N2E2_4398 - ATP-dependent DNA helicase Rep, at 5,126,346 to 5,128,355 _4398 Position (kb) 5123 5124 5125 5126Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5122.783 kb on + strand, within Pf6N2E2_4394at 5122.784 kb on - strand, within Pf6N2E2_4394at 5122.912 kb on + strand, within Pf6N2E2_4394at 5122.912 kb on + strand, within Pf6N2E2_4394at 5122.912 kb on + strand, within Pf6N2E2_4394at 5122.913 kb on - strand, within Pf6N2E2_4394at 5122.913 kb on - strand, within Pf6N2E2_4394at 5122.913 kb on - strand, within Pf6N2E2_4394at 5122.915 kb on + strand, within Pf6N2E2_4394at 5122.915 kb on + strand, within Pf6N2E2_4394at 5122.915 kb on + strand, within Pf6N2E2_4394at 5122.916 kb on - strand, within Pf6N2E2_4394at 5122.916 kb on - strand, within Pf6N2E2_4394at 5122.916 kb on - strand, within Pf6N2E2_4394at 5122.948 kb on - strand, within Pf6N2E2_4394at 5122.974 kb on + strandat 5122.975 kb on - strandat 5123.111 kb on + strandat 5123.113 kb on + strandat 5123.113 kb on + strandat 5123.113 kb on + strandat 5123.152 kb on + strandat 5123.170 kb on + strandat 5123.171 kb on - strandat 5123.182 kb on + strandat 5123.182 kb on + strandat 5123.182 kb on + strandat 5123.182 kb on + strandat 5123.182 kb on + strandat 5123.182 kb on + strandat 5123.182 kb on + strandat 5123.183 kb on - strandat 5123.183 kb on - strandat 5123.183 kb on - strandat 5123.186 kb on + strandat 5123.186 kb on + strandat 5123.186 kb on + strandat 5123.216 kb on - strandat 5123.216 kb on - strandat 5123.225 kb on - strandat 5123.225 kb on - strandat 5123.598 kb on + strandat 5123.598 kb on + strandat 5123.598 kb on + strandat 5123.601 kb on + strandat 5123.602 kb on - strandat 5123.631 kb on + strandat 5123.632 kb on - strandat 5123.799 kb on + strandat 5123.799 kb on + strandat 5123.800 kb on - strandat 5123.800 kb on - strandat 5123.800 kb on - strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.899 kb on + strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.900 kb on - strandat 5123.901 kb on + strandat 5124.004 kb on + strand, within Pf6N2E2_4396at 5124.005 kb on - strand, within Pf6N2E2_4396at 5124.005 kb on - strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.044 kb on + strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.045 kb on - strand, within Pf6N2E2_4396at 5124.109 kb on + strand, within Pf6N2E2_4396at 5124.109 kb on + strand, within Pf6N2E2_4396at 5124.109 kb on + strand, within Pf6N2E2_4396at 5124.109 kb on + strand, within Pf6N2E2_4396at 5124.110 kb on - strand, within Pf6N2E2_4396at 5124.110 kb on - strand, within Pf6N2E2_4396at 5124.110 kb on - strand, within Pf6N2E2_4396at 5124.110 kb on - strand, within Pf6N2E2_4396at 5124.110 kb on - strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.124 kb on + strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.125 kb on - strand, within Pf6N2E2_4396at 5124.325 kb on + strand, within Pf6N2E2_4396at 5124.325 kb on + strand, within Pf6N2E2_4396at 5124.325 kb on + strand, within Pf6N2E2_4396at 5124.325 kb on + strand, within Pf6N2E2_4396at 5124.459 kb on + strand, within Pf6N2E2_4396at 5124.459 kb on + strand, within Pf6N2E2_4396at 5124.649 kb on + strand, within Pf6N2E2_4396at 5124.650 kb on - strand, within Pf6N2E2_4396at 5124.797 kb on + strand, within Pf6N2E2_4396at 5124.798 kb on - strand, within Pf6N2E2_4396at 5124.798 kb on - strand, within Pf6N2E2_4396at 5124.798 kb on - strand, within Pf6N2E2_4396at 5124.832 kb on + strand, within Pf6N2E2_4396at 5124.832 kb on + strand, within Pf6N2E2_4396at 5124.895 kb on + strand, within Pf6N2E2_4396at 5124.895 kb on + strand, within Pf6N2E2_4396at 5124.895 kb on + strand, within Pf6N2E2_4396at 5124.896 kb on - strand, within Pf6N2E2_4396at 5124.896 kb on - strand, within Pf6N2E2_4396at 5124.896 kb on - strand, within Pf6N2E2_4396at 5124.896 kb on - strand, within Pf6N2E2_4396at 5124.896 kb on - strand, within Pf6N2E2_4396at 5124.910 kb on + strand, within Pf6N2E2_4396at 5124.911 kb on - strand, within Pf6N2E2_4396at 5124.963 kb on + strand, within Pf6N2E2_4396at 5124.963 kb on + strand, within Pf6N2E2_4396at 5124.963 kb on + strand, within Pf6N2E2_4396at 5124.963 kb on + strand, within Pf6N2E2_4396at 5124.963 kb on + strand, within Pf6N2E2_4396at 5125.048 kb on + strand, within Pf6N2E2_4396at 5125.049 kb on - strand, within Pf6N2E2_4396at 5125.049 kb on - strand, within Pf6N2E2_4396at 5125.144 kb on - strand, within Pf6N2E2_4396at 5125.156 kb on + strand, within Pf6N2E2_4396at 5125.208 kb on - strand, within Pf6N2E2_4396at 5125.448 kb on + strand, within Pf6N2E2_4396at 5125.449 kb on - strand, within Pf6N2E2_4396at 5125.449 kb on - strand, within Pf6N2E2_4396at 5125.704 kb on + strandat 5125.704 kb on + strandat 5125.704 kb on + strandat 5125.705 kb on - strandat 5125.705 kb on - strandat 5125.705 kb on - strandat 5125.705 kb on - strandat 5125.705 kb on - strandat 5125.999 kb on + strand, within Pf6N2E2_4397at 5125.999 kb on + strand, within Pf6N2E2_4397at 5126.000 kb on - strand, within Pf6N2E2_4397at 5126.000 kb on - strand, within Pf6N2E2_4397at 5126.348 kb on - strandat 5126.356 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM
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5,122,783 + Pf6N2E2_4394 0.60 -0.8
5,122,784 - Pf6N2E2_4394 0.60 -1.3
5,122,912 + Pf6N2E2_4394 0.83 -1.3
5,122,912 + Pf6N2E2_4394 0.83 +0.1
5,122,912 + Pf6N2E2_4394 0.83 -0.5
5,122,913 - Pf6N2E2_4394 0.83 -0.8
5,122,913 - Pf6N2E2_4394 0.83 +1.5
5,122,913 - Pf6N2E2_4394 0.83 -1.1
5,122,915 + Pf6N2E2_4394 0.84 -1.1
5,122,915 + Pf6N2E2_4394 0.84 -1.8
5,122,915 + Pf6N2E2_4394 0.84 -0.1
5,122,916 - Pf6N2E2_4394 0.84 -1.3
5,122,916 - Pf6N2E2_4394 0.84 -1.1
5,122,916 - Pf6N2E2_4394 0.84 -2.4
5,122,948 - Pf6N2E2_4394 0.90 -0.5
5,122,974 + -1.1
5,122,975 - -1.5
5,123,111 + +0.5
5,123,113 + -0.5
5,123,113 + -0.3
5,123,113 + +0.5
5,123,152 + -1.8
5,123,170 + +1.3
5,123,171 - -0.7
5,123,182 + +0.5
5,123,182 + -1.2
5,123,182 + -0.6
5,123,182 + -0.6
5,123,182 + -0.6
5,123,182 + +0.1
5,123,182 + -0.7
5,123,183 - -0.2
5,123,183 - -0.6
5,123,183 - -0.7
5,123,186 + -0.0
5,123,186 + -0.7
5,123,186 + +0.2
5,123,216 - -0.2
5,123,216 - -1.7
5,123,225 - +0.0
5,123,225 - -1.2
5,123,598 + -0.6
5,123,598 + -0.8
5,123,598 + -0.9
5,123,601 + -1.7
5,123,602 - +0.7
5,123,631 + +0.7
5,123,632 - -1.1
5,123,799 + +0.2
5,123,799 + +1.7
5,123,800 - -2.1
5,123,800 - -1.3
5,123,800 - -1.8
5,123,899 + -0.5
5,123,899 + -0.3
5,123,899 + +0.3
5,123,899 + -0.3
5,123,899 + -0.3
5,123,899 + +0.3
5,123,899 + -0.2
5,123,899 + -0.8
5,123,900 - -0.8
5,123,900 - +0.2
5,123,900 - +1.5
5,123,900 - -1.3
5,123,900 - -2.7
5,123,900 - -3.3
5,123,900 - -0.1
5,123,900 - -1.7
5,123,900 - -0.4
5,123,900 - -1.8
5,123,900 - -1.2
5,123,900 - -2.2
5,123,900 - -1.2
5,123,900 - -0.3
5,123,900 - -0.5
5,123,900 - -2.8
5,123,900 - -0.5
5,123,900 - -0.9
5,123,900 - -0.6
5,123,900 - +0.4
5,123,900 - -0.5
5,123,900 - -0.1
5,123,900 - -0.6
5,123,900 - -0.5
5,123,901 + -0.2
5,124,004 + Pf6N2E2_4396 0.14 +0.8
5,124,005 - Pf6N2E2_4396 0.14 -0.6
5,124,005 - Pf6N2E2_4396 0.14 -0.6
5,124,044 + Pf6N2E2_4396 0.16 -0.5
5,124,044 + Pf6N2E2_4396 0.16 +0.2
5,124,044 + Pf6N2E2_4396 0.16 -0.0
5,124,044 + Pf6N2E2_4396 0.16 -0.9
5,124,044 + Pf6N2E2_4396 0.16 +0.5
5,124,044 + Pf6N2E2_4396 0.16 -0.8
5,124,044 + Pf6N2E2_4396 0.16 +1.9
5,124,044 + Pf6N2E2_4396 0.16 -0.1
5,124,045 - Pf6N2E2_4396 0.16 -0.7
5,124,045 - Pf6N2E2_4396 0.16 -1.7
5,124,045 - Pf6N2E2_4396 0.16 -0.1
5,124,045 - Pf6N2E2_4396 0.16 -0.7
5,124,045 - Pf6N2E2_4396 0.16 -0.4
5,124,045 - Pf6N2E2_4396 0.16 -1.3
5,124,045 - Pf6N2E2_4396 0.16 -1.3
5,124,109 + Pf6N2E2_4396 0.19 +0.2
5,124,109 + Pf6N2E2_4396 0.19 -0.3
5,124,109 + Pf6N2E2_4396 0.19 +0.1
5,124,109 + Pf6N2E2_4396 0.19 -0.8
5,124,110 - Pf6N2E2_4396 0.19 -2.2
5,124,110 - Pf6N2E2_4396 0.19 -1.1
5,124,110 - Pf6N2E2_4396 0.19 -1.1
5,124,110 - Pf6N2E2_4396 0.19 -0.2
5,124,110 - Pf6N2E2_4396 0.19 -0.2
5,124,124 + Pf6N2E2_4396 0.20 +0.8
5,124,124 + Pf6N2E2_4396 0.20 -2.2
5,124,124 + Pf6N2E2_4396 0.20 -1.1
5,124,124 + Pf6N2E2_4396 0.20 -0.6
5,124,124 + Pf6N2E2_4396 0.20 +0.5
5,124,124 + Pf6N2E2_4396 0.20 +2.0
5,124,124 + Pf6N2E2_4396 0.20 -0.1
5,124,124 + Pf6N2E2_4396 0.20 +1.2
5,124,124 + Pf6N2E2_4396 0.20 +1.5
5,124,124 + Pf6N2E2_4396 0.20 -0.2
5,124,125 - Pf6N2E2_4396 0.20 -0.8
5,124,125 - Pf6N2E2_4396 0.20 -0.1
5,124,125 - Pf6N2E2_4396 0.20 -3.0
5,124,125 - Pf6N2E2_4396 0.20 +1.1
5,124,125 - Pf6N2E2_4396 0.20 +0.5
5,124,125 - Pf6N2E2_4396 0.20 -1.7
5,124,125 - Pf6N2E2_4396 0.20 -1.1
5,124,325 + Pf6N2E2_4396 0.31 -0.7
5,124,325 + Pf6N2E2_4396 0.31 -1.2
5,124,325 + Pf6N2E2_4396 0.31 -0.6
5,124,325 + Pf6N2E2_4396 0.31 +0.2
5,124,459 + Pf6N2E2_4396 0.38 -0.6
5,124,459 + Pf6N2E2_4396 0.38 -0.2
5,124,649 + Pf6N2E2_4396 0.47 -1.3
5,124,650 - Pf6N2E2_4396 0.47 -0.7
5,124,797 + Pf6N2E2_4396 0.55 -1.6
5,124,798 - Pf6N2E2_4396 0.55 -0.9
5,124,798 - Pf6N2E2_4396 0.55 -0.1
5,124,798 - Pf6N2E2_4396 0.55 +1.2
5,124,832 + Pf6N2E2_4396 0.57 +0.0
5,124,832 + Pf6N2E2_4396 0.57 +0.5
5,124,895 + Pf6N2E2_4396 0.60 +1.5
5,124,895 + Pf6N2E2_4396 0.60 -0.7
5,124,895 + Pf6N2E2_4396 0.60 +0.4
5,124,896 - Pf6N2E2_4396 0.60 -1.3
5,124,896 - Pf6N2E2_4396 0.60 +0.3
5,124,896 - Pf6N2E2_4396 0.60 -0.5
5,124,896 - Pf6N2E2_4396 0.60 -0.2
5,124,896 - Pf6N2E2_4396 0.60 -1.0
5,124,910 + Pf6N2E2_4396 0.61 -1.2
5,124,911 - Pf6N2E2_4396 0.61 -0.6
5,124,963 + Pf6N2E2_4396 0.64 -0.4
5,124,963 + Pf6N2E2_4396 0.64 -0.3
5,124,963 + Pf6N2E2_4396 0.64 +0.2
5,124,963 + Pf6N2E2_4396 0.64 +1.1
5,124,963 + Pf6N2E2_4396 0.64 -2.6
5,125,048 + Pf6N2E2_4396 0.68 +0.7
5,125,049 - Pf6N2E2_4396 0.68 -2.4
5,125,049 - Pf6N2E2_4396 0.68 -1.2
5,125,144 - Pf6N2E2_4396 0.73 -0.5
5,125,156 + Pf6N2E2_4396 0.74 -0.5
5,125,208 - Pf6N2E2_4396 0.76 +0.6
5,125,448 + Pf6N2E2_4396 0.89 -0.1
5,125,449 - Pf6N2E2_4396 0.89 -2.0
5,125,449 - Pf6N2E2_4396 0.89 -0.9
5,125,704 + +0.2
5,125,704 + -0.8
5,125,704 + -2.0
5,125,705 - -0.3
5,125,705 - +1.5
5,125,705 - +0.2
5,125,705 - -2.3
5,125,705 - -0.3
5,125,999 + Pf6N2E2_4397 0.49 -0.7
5,125,999 + Pf6N2E2_4397 0.49 +0.1
5,126,000 - Pf6N2E2_4397 0.50 +0.7
5,126,000 - Pf6N2E2_4397 0.50 -0.2
5,126,348 - -1.3
5,126,356 - +1.5

Or see this region's nucleotide sequence