Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_773 and Pf6N2E2_774 overlap by 4 nucleotides Pf6N2E2_774 and Pf6N2E2_775 are separated by 4 nucleotides Pf6N2E2_775 and Pf6N2E2_776 overlap by 4 nucleotides Pf6N2E2_776 and Pf6N2E2_777 are separated by 128 nucleotides Pf6N2E2_777 and Pf6N2E2_778 are separated by 76 nucleotides
Pf6N2E2_773 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 884,233 to 885,063
_773
Pf6N2E2_774 - Cold-shock DNA-binding domain, at 885,060 to 885,479
_774
Pf6N2E2_775 - FIG00955061: hypothetical protein, at 885,484 to 885,834
_775
Pf6N2E2_776 - Transcriptional regulator, LysR family, at 885,831 to 886,739
_776
Pf6N2E2_777 - FMN-dependent NADH-azoreductase, at 886,868 to 887,476
_777
Pf6N2E2_778 - Probable lipoprotein signal peptide, at 887,553 to 888,698
_778
Position (kb)
885
886
887 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 884.837 kb on - strand, within Pf6N2E2_773 at 884.846 kb on - strand, within Pf6N2E2_773 at 884.846 kb on - strand, within Pf6N2E2_773 at 884.851 kb on + strand, within Pf6N2E2_773 at 884.968 kb on + strand, within Pf6N2E2_773 at 884.968 kb on + strand, within Pf6N2E2_773 at 884.968 kb on + strand, within Pf6N2E2_773 at 884.969 kb on - strand, within Pf6N2E2_773 at 884.969 kb on - strand, within Pf6N2E2_773 at 884.969 kb on - strand, within Pf6N2E2_773 at 884.969 kb on - strand, within Pf6N2E2_773 at 884.969 kb on - strand, within Pf6N2E2_773 at 884.969 kb on - strand, within Pf6N2E2_773 at 885.469 kb on - strand at 885.485 kb on + strand at 885.486 kb on - strand at 885.486 kb on - strand at 885.624 kb on - strand, within Pf6N2E2_775 at 885.770 kb on + strand, within Pf6N2E2_775 at 885.771 kb on - strand, within Pf6N2E2_775 at 885.771 kb on - strand, within Pf6N2E2_775 at 885.771 kb on - strand, within Pf6N2E2_775 at 885.771 kb on - strand, within Pf6N2E2_775 at 885.771 kb on - strand, within Pf6N2E2_775 at 885.834 kb on + strand at 885.842 kb on + strand at 885.843 kb on - strand at 885.843 kb on - strand at 885.843 kb on - strand at 886.006 kb on + strand, within Pf6N2E2_776 at 886.006 kb on + strand, within Pf6N2E2_776 at 886.006 kb on + strand, within Pf6N2E2_776 at 886.006 kb on + strand, within Pf6N2E2_776 at 886.006 kb on + strand, within Pf6N2E2_776 at 886.006 kb on + strand at 886.006 kb on + strand, within Pf6N2E2_776 at 886.007 kb on - strand, within Pf6N2E2_776 at 886.007 kb on - strand, within Pf6N2E2_776 at 886.007 kb on - strand, within Pf6N2E2_776 at 886.010 kb on + strand, within Pf6N2E2_776 at 886.010 kb on + strand, within Pf6N2E2_776 at 886.011 kb on - strand, within Pf6N2E2_776 at 886.020 kb on + strand, within Pf6N2E2_776 at 886.367 kb on + strand, within Pf6N2E2_776 at 886.367 kb on + strand, within Pf6N2E2_776 at 886.388 kb on - strand, within Pf6N2E2_776 at 886.410 kb on + strand, within Pf6N2E2_776 at 886.546 kb on + strand, within Pf6N2E2_776 at 886.546 kb on + strand, within Pf6N2E2_776 at 886.714 kb on + strand at 886.714 kb on + strand at 886.714 kb on + strand at 886.717 kb on + strand at 886.717 kb on + strand at 886.718 kb on - strand at 886.718 kb on - strand at 886.773 kb on + strand at 886.774 kb on - strand at 886.811 kb on - strand at 886.814 kb on + strand at 886.814 kb on + strand at 886.868 kb on - strand at 886.876 kb on - strand at 886.978 kb on + strand, within Pf6N2E2_777 at 886.983 kb on - strand, within Pf6N2E2_777 at 886.983 kb on - strand, within Pf6N2E2_777 at 887.045 kb on - strand, within Pf6N2E2_777 at 887.092 kb on + strand, within Pf6N2E2_777 at 887.153 kb on + strand, within Pf6N2E2_777 at 887.154 kb on - strand, within Pf6N2E2_777 at 887.333 kb on + strand, within Pf6N2E2_777 at 887.333 kb on + strand, within Pf6N2E2_777 at 887.333 kb on + strand, within Pf6N2E2_777 at 887.334 kb on - strand, within Pf6N2E2_777 at 887.359 kb on - strand, within Pf6N2E2_777 at 887.423 kb on - strand at 887.486 kb on - strand at 887.486 kb on - strand at 887.516 kb on + strand at 887.516 kb on + strand at 887.516 kb on + strand at 887.517 kb on - strand at 887.527 kb on - strand at 887.565 kb on - strand at 887.565 kb on - strand at 887.565 kb on - strand at 887.599 kb on - strand at 887.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 884,837 - Pf6N2E2_773 0.73 -0.4 884,846 - Pf6N2E2_773 0.74 +0.8 884,846 - Pf6N2E2_773 0.74 +0.4 884,851 + Pf6N2E2_773 0.74 +1.0 884,968 + Pf6N2E2_773 0.88 +0.3 884,968 + Pf6N2E2_773 0.88 +0.8 884,968 + Pf6N2E2_773 0.88 +0.9 884,969 - Pf6N2E2_773 0.89 -1.1 884,969 - Pf6N2E2_773 0.89 +0.7 884,969 - Pf6N2E2_773 0.89 +0.1 884,969 - Pf6N2E2_773 0.89 -1.0 884,969 - Pf6N2E2_773 0.89 +0.6 884,969 - Pf6N2E2_773 0.89 -1.4 885,469 - +1.9 885,485 + -0.5 885,486 - -2.7 885,486 - +0.9 885,624 - Pf6N2E2_775 0.40 -2.8 885,770 + Pf6N2E2_775 0.81 -0.1 885,771 - Pf6N2E2_775 0.82 -2.5 885,771 - Pf6N2E2_775 0.82 -0.7 885,771 - Pf6N2E2_775 0.82 -0.7 885,771 - Pf6N2E2_775 0.82 -0.6 885,771 - Pf6N2E2_775 0.82 +0.4 885,834 + +0.9 885,842 + -1.9 885,843 - -2.0 885,843 - -0.7 885,843 - +0.5 886,006 + Pf6N2E2_776 0.19 +0.3 886,006 + Pf6N2E2_776 0.19 +1.9 886,006 + Pf6N2E2_776 0.19 +1.9 886,006 + Pf6N2E2_776 0.19 -2.5 886,006 + Pf6N2E2_776 0.19 -0.7 886,006 + -1.2 886,006 + Pf6N2E2_776 0.19 +0.7 886,007 - Pf6N2E2_776 0.19 -1.0 886,007 - Pf6N2E2_776 0.19 -1.2 886,007 - Pf6N2E2_776 0.19 +1.9 886,010 + Pf6N2E2_776 0.20 -0.7 886,010 + Pf6N2E2_776 0.20 +1.9 886,011 - Pf6N2E2_776 0.20 -0.4 886,020 + Pf6N2E2_776 0.21 +1.9 886,367 + Pf6N2E2_776 0.59 -0.1 886,367 + Pf6N2E2_776 0.59 -1.7 886,388 - Pf6N2E2_776 0.61 -0.1 886,410 + Pf6N2E2_776 0.64 -0.7 886,546 + Pf6N2E2_776 0.79 -0.4 886,546 + Pf6N2E2_776 0.79 -0.4 886,714 + -0.1 886,714 + +0.1 886,714 + -0.1 886,717 + +1.9 886,717 + -0.5 886,718 - +1.9 886,718 - -2.9 886,773 + -0.2 886,774 - -0.4 886,811 - +0.2 886,814 + -2.2 886,814 + +1.7 886,868 - +1.9 886,876 - +0.3 886,978 + Pf6N2E2_777 0.18 -0.1 886,983 - Pf6N2E2_777 0.19 +1.0 886,983 - Pf6N2E2_777 0.19 +0.3 887,045 - Pf6N2E2_777 0.29 -2.1 887,092 + Pf6N2E2_777 0.37 -0.6 887,153 + Pf6N2E2_777 0.47 +0.9 887,154 - Pf6N2E2_777 0.47 -0.7 887,333 + Pf6N2E2_777 0.76 +1.9 887,333 + Pf6N2E2_777 0.76 -1.3 887,333 + Pf6N2E2_777 0.76 +0.4 887,334 - Pf6N2E2_777 0.77 -1.0 887,359 - Pf6N2E2_777 0.81 +0.3 887,423 - +0.7 887,486 - +0.8 887,486 - -0.5 887,516 + -0.7 887,516 + +0.2 887,516 + -0.3 887,517 - -0.4 887,527 - +1.0 887,565 - +0.2 887,565 - -0.6 887,565 - -1.5 887,599 - +0.0 887,666 - +1.9
Or see this region's nucleotide sequence