Strain Fitness in Escherichia coli BW25113 around b2072

Experiment: N4_phage 0.00009375 MOI TC1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegI and yegJ are separated by 199 nucleotidesyegJ and yegK are separated by 64 nucleotidesyegK and yegL overlap by 4 nucleotidesyegL and ryeC are separated by 180 nucleotidesryeC and ibsA overlap by 103 nucleotides b2070: yegI - hypothetical protein (NCBI), at 2,147,063 to 2,149,009 yegI b2071: yegJ - hypothetical protein (NCBI), at 2,149,209 to 2,149,670 yegJ b2072: yegK - hypothetical protein (NCBI), at 2,149,735 to 2,150,496 yegK b2073: yegL - hypothetical protein (NCBI), at 2,150,493 to 2,151,152 yegL b4436: ryeC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq), at 2,151,333 to 2,151,475 ryeC b4667: ibsA - toxic membrane protein (RefSeq), at 2,151,373 to 2,151,432 ibsA Position (kb) 2149 2150 2151Strain fitness (log2 ratio) -2 -1 0 1 2at 2148.766 kb on + strand, within yegIat 2148.774 kb on + strand, within yegIat 2148.777 kb on - strand, within yegIat 2148.782 kb on + strand, within yegIat 2148.782 kb on + strand, within yegIat 2148.854 kb on - strandat 2148.932 kb on + strandat 2148.934 kb on + strandat 2148.934 kb on + strandat 2148.939 kb on + strandat 2148.949 kb on + strandat 2148.949 kb on + strandat 2148.955 kb on + strandat 2148.956 kb on + strandat 2148.956 kb on + strandat 2148.956 kb on + strandat 2148.973 kb on + strandat 2149.410 kb on + strand, within yegJat 2149.433 kb on + strand, within yegJat 2149.438 kb on + strand, within yegJat 2149.575 kb on - strand, within yegJat 2149.632 kb on - strandat 2149.660 kb on - strandat 2149.660 kb on - strandat 2149.757 kb on - strandat 2149.757 kb on - strandat 2149.776 kb on - strandat 2149.776 kb on - strandat 2149.901 kb on + strand, within yegKat 2149.909 kb on - strand, within yegKat 2149.909 kb on - strand, within yegKat 2149.973 kb on + strand, within yegKat 2149.981 kb on - strand, within yegKat 2150.081 kb on - strand, within yegKat 2150.081 kb on - strand, within yegKat 2150.299 kb on - strand, within yegKat 2150.330 kb on + strand, within yegKat 2150.330 kb on + strand, within yegKat 2150.338 kb on - strand, within yegKat 2150.633 kb on + strand, within yegLat 2150.650 kb on + strand, within yegLat 2150.753 kb on + strand, within yegLat 2150.819 kb on - strand, within yegLat 2150.819 kb on - strand, within yegLat 2150.836 kb on + strand, within yegLat 2150.836 kb on + strand, within yegLat 2150.846 kb on - strand, within yegLat 2150.846 kb on - strand, within yegLat 2150.856 kb on + strand, within yegLat 2150.863 kb on + strand, within yegLat 2150.865 kb on + strand, within yegLat 2150.891 kb on - strand, within yegLat 2150.919 kb on + strand, within yegLat 2150.919 kb on + strand, within yegLat 2150.943 kb on - strand, within yegLat 2150.943 kb on - strand, within yegLat 2150.962 kb on - strand, within yegLat 2151.035 kb on + strand, within yegLat 2151.046 kb on - strand, within yegLat 2151.104 kb on - strandat 2151.104 kb on - strandat 2151.195 kb on - strandat 2151.277 kb on + strandat 2151.299 kb on - strandat 2151.311 kb on - strandat 2151.339 kb on + strandat 2151.339 kb on - strandat 2151.354 kb on + strand, within ryeCat 2151.354 kb on + strand, within ryeCat 2151.388 kb on - strandat 2151.411 kb on + strandat 2151.411 kb on + strandat 2151.432 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC1
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2,148,766 + yegI b2070 0.87 -0.4
2,148,774 + yegI b2070 0.88 -0.4
2,148,777 - yegI b2070 0.88 +0.2
2,148,782 + yegI b2070 0.88 -0.8
2,148,782 + yegI b2070 0.88 -0.1
2,148,854 - -0.1
2,148,932 + -0.3
2,148,934 + -0.3
2,148,934 + +1.5
2,148,939 + +1.2
2,148,949 + -0.8
2,148,949 + +1.9
2,148,955 + +2.2
2,148,956 + -1.3
2,148,956 + +0.6
2,148,956 + -0.8
2,148,973 + +0.9
2,149,410 + yegJ b2071 0.44 +2.2
2,149,433 + yegJ b2071 0.48 +0.6
2,149,438 + yegJ b2071 0.50 +2.2
2,149,575 - yegJ b2071 0.79 +1.2
2,149,632 - +1.2
2,149,660 - -0.7
2,149,660 - +0.8
2,149,757 - +1.7
2,149,757 - +1.0
2,149,776 - -0.1
2,149,776 - -1.7
2,149,901 + yegK b2072 0.22 +2.2
2,149,909 - yegK b2072 0.23 -0.6
2,149,909 - yegK b2072 0.23 -0.8
2,149,973 + yegK b2072 0.31 -2.5
2,149,981 - yegK b2072 0.32 -0.4
2,150,081 - yegK b2072 0.45 -2.1
2,150,081 - yegK b2072 0.45 +0.0
2,150,299 - yegK b2072 0.74 +0.1
2,150,330 + yegK b2072 0.78 +0.3
2,150,330 + yegK b2072 0.78 -0.6
2,150,338 - yegK b2072 0.79 -1.6
2,150,633 + yegL b2073 0.21 +0.9
2,150,650 + yegL b2073 0.24 -0.7
2,150,753 + yegL b2073 0.39 -0.1
2,150,819 - yegL b2073 0.49 -0.3
2,150,819 - yegL b2073 0.49 +0.4
2,150,836 + yegL b2073 0.52 +0.1
2,150,836 + yegL b2073 0.52 +0.9
2,150,846 - yegL b2073 0.53 -0.2
2,150,846 - yegL b2073 0.53 +2.1
2,150,856 + yegL b2073 0.55 +0.1
2,150,863 + yegL b2073 0.56 -0.5
2,150,865 + yegL b2073 0.56 -0.4
2,150,891 - yegL b2073 0.60 -0.4
2,150,919 + yegL b2073 0.65 -1.6
2,150,919 + yegL b2073 0.65 -0.7
2,150,943 - yegL b2073 0.68 -0.7
2,150,943 - yegL b2073 0.68 -0.8
2,150,962 - yegL b2073 0.71 +0.8
2,151,035 + yegL b2073 0.82 -0.8
2,151,046 - yegL b2073 0.84 -0.4
2,151,104 - -0.7
2,151,104 - -1.8
2,151,195 - +0.2
2,151,277 + +0.1
2,151,299 - +0.3
2,151,311 - +0.1
2,151,339 + +0.0
2,151,339 - -0.2
2,151,354 + ryeC b4436 0.15 +0.3
2,151,354 + ryeC b4436 0.15 +0.5
2,151,388 - +0.0
2,151,411 + -0.1
2,151,411 + +0.7
2,151,432 - +0.2

Or see this region's nucleotide sequence