Experiment: N4_phage 0.00009375 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegI and yegJ are separated by 199 nucleotides yegJ and yegK are separated by 64 nucleotides yegK and yegL overlap by 4 nucleotides yegL and ryeC are separated by 180 nucleotides ryeC and ibsA overlap by 103 nucleotides
b2070: yegI - hypothetical protein (NCBI), at 2,147,063 to 2,149,009
yegI
b2071: yegJ - hypothetical protein (NCBI), at 2,149,209 to 2,149,670
yegJ
b2072: yegK - hypothetical protein (NCBI), at 2,149,735 to 2,150,496
yegK
b2073: yegL - hypothetical protein (NCBI), at 2,150,493 to 2,151,152
yegL
b4436: ryeC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq), at 2,151,333 to 2,151,475
ryeC
b4667: ibsA - toxic membrane protein (RefSeq), at 2,151,373 to 2,151,432
ibsA
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2148.766 kb on + strand, within yegI at 2148.774 kb on + strand, within yegI at 2148.777 kb on - strand, within yegI at 2148.782 kb on + strand, within yegI at 2148.782 kb on + strand, within yegI at 2148.854 kb on - strand at 2148.932 kb on + strand at 2148.934 kb on + strand at 2148.934 kb on + strand at 2148.939 kb on + strand at 2148.949 kb on + strand at 2148.949 kb on + strand at 2148.955 kb on + strand at 2148.956 kb on + strand at 2148.956 kb on + strand at 2148.956 kb on + strand at 2148.973 kb on + strand at 2149.410 kb on + strand, within yegJ at 2149.433 kb on + strand, within yegJ at 2149.438 kb on + strand, within yegJ at 2149.575 kb on - strand, within yegJ at 2149.632 kb on - strand at 2149.660 kb on - strand at 2149.660 kb on - strand at 2149.757 kb on - strand at 2149.757 kb on - strand at 2149.776 kb on - strand at 2149.776 kb on - strand at 2149.901 kb on + strand, within yegK at 2149.909 kb on - strand, within yegK at 2149.909 kb on - strand, within yegK at 2149.973 kb on + strand, within yegK at 2149.981 kb on - strand, within yegK at 2150.081 kb on - strand, within yegK at 2150.081 kb on - strand, within yegK at 2150.299 kb on - strand, within yegK at 2150.330 kb on + strand, within yegK at 2150.330 kb on + strand, within yegK at 2150.338 kb on - strand, within yegK at 2150.633 kb on + strand, within yegL at 2150.650 kb on + strand, within yegL at 2150.753 kb on + strand, within yegL at 2150.819 kb on - strand, within yegL at 2150.819 kb on - strand, within yegL at 2150.836 kb on + strand, within yegL at 2150.836 kb on + strand, within yegL at 2150.846 kb on - strand, within yegL at 2150.846 kb on - strand, within yegL at 2150.856 kb on + strand, within yegL at 2150.863 kb on + strand, within yegL at 2150.865 kb on + strand, within yegL at 2150.891 kb on - strand, within yegL at 2150.919 kb on + strand, within yegL at 2150.919 kb on + strand, within yegL at 2150.943 kb on - strand, within yegL at 2150.943 kb on - strand, within yegL at 2150.962 kb on - strand, within yegL at 2151.035 kb on + strand, within yegL at 2151.046 kb on - strand, within yegL at 2151.104 kb on - strand at 2151.104 kb on - strand at 2151.195 kb on - strand at 2151.277 kb on + strand at 2151.299 kb on - strand at 2151.311 kb on - strand at 2151.339 kb on + strand at 2151.339 kb on - strand at 2151.354 kb on + strand, within ryeC at 2151.354 kb on + strand, within ryeC at 2151.388 kb on - strand at 2151.411 kb on + strand at 2151.411 kb on + strand at 2151.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC1 remove 2,148,766 + yegI b2070 0.87 -0.4 2,148,774 + yegI b2070 0.88 -0.4 2,148,777 - yegI b2070 0.88 +0.2 2,148,782 + yegI b2070 0.88 -0.8 2,148,782 + yegI b2070 0.88 -0.1 2,148,854 - -0.1 2,148,932 + -0.3 2,148,934 + -0.3 2,148,934 + +1.5 2,148,939 + +1.2 2,148,949 + -0.8 2,148,949 + +1.9 2,148,955 + +2.2 2,148,956 + -1.3 2,148,956 + +0.6 2,148,956 + -0.8 2,148,973 + +0.9 2,149,410 + yegJ b2071 0.44 +2.2 2,149,433 + yegJ b2071 0.48 +0.6 2,149,438 + yegJ b2071 0.50 +2.2 2,149,575 - yegJ b2071 0.79 +1.2 2,149,632 - +1.2 2,149,660 - -0.7 2,149,660 - +0.8 2,149,757 - +1.7 2,149,757 - +1.0 2,149,776 - -0.1 2,149,776 - -1.7 2,149,901 + yegK b2072 0.22 +2.2 2,149,909 - yegK b2072 0.23 -0.6 2,149,909 - yegK b2072 0.23 -0.8 2,149,973 + yegK b2072 0.31 -2.5 2,149,981 - yegK b2072 0.32 -0.4 2,150,081 - yegK b2072 0.45 -2.1 2,150,081 - yegK b2072 0.45 +0.0 2,150,299 - yegK b2072 0.74 +0.1 2,150,330 + yegK b2072 0.78 +0.3 2,150,330 + yegK b2072 0.78 -0.6 2,150,338 - yegK b2072 0.79 -1.6 2,150,633 + yegL b2073 0.21 +0.9 2,150,650 + yegL b2073 0.24 -0.7 2,150,753 + yegL b2073 0.39 -0.1 2,150,819 - yegL b2073 0.49 -0.3 2,150,819 - yegL b2073 0.49 +0.4 2,150,836 + yegL b2073 0.52 +0.1 2,150,836 + yegL b2073 0.52 +0.9 2,150,846 - yegL b2073 0.53 -0.2 2,150,846 - yegL b2073 0.53 +2.1 2,150,856 + yegL b2073 0.55 +0.1 2,150,863 + yegL b2073 0.56 -0.5 2,150,865 + yegL b2073 0.56 -0.4 2,150,891 - yegL b2073 0.60 -0.4 2,150,919 + yegL b2073 0.65 -1.6 2,150,919 + yegL b2073 0.65 -0.7 2,150,943 - yegL b2073 0.68 -0.7 2,150,943 - yegL b2073 0.68 -0.8 2,150,962 - yegL b2073 0.71 +0.8 2,151,035 + yegL b2073 0.82 -0.8 2,151,046 - yegL b2073 0.84 -0.4 2,151,104 - -0.7 2,151,104 - -1.8 2,151,195 - +0.2 2,151,277 + +0.1 2,151,299 - +0.3 2,151,311 - +0.1 2,151,339 + +0.0 2,151,339 - -0.2 2,151,354 + ryeC b4436 0.15 +0.3 2,151,354 + ryeC b4436 0.15 +0.5 2,151,388 - +0.0 2,151,411 + -0.1 2,151,411 + +0.7 2,151,432 - +0.2
Or see this region's nucleotide sequence