Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33545 and BPHYT_RS33550 are separated by 79 nucleotides BPHYT_RS33550 and BPHYT_RS33555 are separated by 234 nucleotides BPHYT_RS33555 and BPHYT_RS33560 are separated by 68 nucleotides
BPHYT_RS33545: BPHYT_RS33545 - DNA primase, at 3,110,401 to 3,112,272
_RS33545
BPHYT_RS33550: BPHYT_RS33550 - aspartyl-tRNA amidotransferase subunit B, at 3,112,352 to 3,112,798
_RS33550
BPHYT_RS33555: BPHYT_RS33555 - 30S ribosomal protein S21, at 3,113,033 to 3,113,245
_RS33555
BPHYT_RS33560: BPHYT_RS33560 - membrane protein, at 3,113,314 to 3,114,531
_RS33560
Position (kb)
3113
3114 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3112.276 kb on - strand at 3112.331 kb on - strand at 3112.350 kb on - strand at 3112.354 kb on - strand at 3112.534 kb on - strand, within BPHYT_RS33550 at 3112.534 kb on - strand, within BPHYT_RS33550 at 3112.769 kb on - strand at 3113.063 kb on - strand, within BPHYT_RS33555 at 3113.105 kb on - strand, within BPHYT_RS33555 at 3113.105 kb on - strand, within BPHYT_RS33555 at 3113.105 kb on - strand, within BPHYT_RS33555 at 3113.123 kb on - strand, within BPHYT_RS33555 at 3113.281 kb on - strand at 3113.281 kb on - strand at 3113.432 kb on + strand at 3113.432 kb on + strand at 3113.432 kb on + strand at 3113.432 kb on + strand at 3113.783 kb on + strand, within BPHYT_RS33560 at 3113.783 kb on + strand, within BPHYT_RS33560 at 3113.783 kb on + strand, within BPHYT_RS33560 at 3113.784 kb on - strand, within BPHYT_RS33560 at 3114.008 kb on + strand, within BPHYT_RS33560 at 3114.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 3,112,276 - -0.6 3,112,331 - +0.7 3,112,350 - -0.7 3,112,354 - +0.7 3,112,534 - BPHYT_RS33550 0.41 -1.8 3,112,534 - BPHYT_RS33550 0.41 -1.4 3,112,769 - +0.5 3,113,063 - BPHYT_RS33555 0.14 +0.0 3,113,105 - BPHYT_RS33555 0.34 -0.4 3,113,105 - BPHYT_RS33555 0.34 +0.2 3,113,105 - BPHYT_RS33555 0.34 +0.9 3,113,123 - BPHYT_RS33555 0.42 +4.9 3,113,281 - -0.1 3,113,281 - -0.6 3,113,432 + -0.6 3,113,432 + +0.6 3,113,432 + +0.1 3,113,432 + -0.1 3,113,783 + BPHYT_RS33560 0.39 -1.7 3,113,783 + BPHYT_RS33560 0.39 +1.5 3,113,783 + BPHYT_RS33560 0.39 -0.2 3,113,784 - BPHYT_RS33560 0.39 +0.5 3,114,008 + BPHYT_RS33560 0.57 -0.2 3,114,096 + -1.3
Or see this region's nucleotide sequence