Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_522

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotidesPf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotidesPf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotidesPf6N2E2_522 and Pf6N2E2_523 are separated by 77 nucleotides Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593 _519 Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720 _520 Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888 _521 Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866 _522 Pf6N2E2_523 - Inositol transport system ATP-binding protein, at 591,944 to 593,497 _523 Position (kb) 590 591 592Strain fitness (log2 ratio) -1 0 1 2 3at 589.998 kb on - strand, within Pf6N2E2_519at 590.121 kb on - strand, within Pf6N2E2_519at 590.261 kb on + strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.429 kb on + strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.724 kb on + strandat 590.724 kb on + strandat 590.724 kb on + strandat 590.724 kb on + strandat 590.725 kb on - strandat 590.729 kb on - strandat 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.764 kb on - strand, within Pf6N2E2_521at 590.764 kb on - strand, within Pf6N2E2_521at 590.907 kb on + strandat 591.060 kb on + strand, within Pf6N2E2_522at 591.061 kb on - strand, within Pf6N2E2_522at 591.061 kb on - strand, within Pf6N2E2_522at 591.478 kb on - strand, within Pf6N2E2_522at 591.658 kb on + strand, within Pf6N2E2_522at 591.770 kb on + strand, within Pf6N2E2_522at 591.770 kb on + strand, within Pf6N2E2_522at 591.897 kb on - strandat 591.935 kb on + strandat 591.935 kb on + strandat 591.935 kb on + strandat 591.999 kb on + strandat 591.999 kb on + strandat 591.999 kb on + strandat 591.999 kb on + strandat 591.999 kb on + strandat 591.999 kb on + strandat 592.000 kb on - strandat 592.000 kb on - strandat 592.002 kb on + strandat 592.003 kb on - strandat 592.003 kb on - strandat 592.003 kb on - strandat 592.017 kb on - strandat 592.017 kb on - strandat 592.077 kb on + strandat 592.077 kb on + strandat 592.077 kb on + strandat 592.077 kb on + strandat 592.077 kb on + strandat 592.077 kb on + strandat 592.078 kb on - strandat 592.169 kb on + strand, within Pf6N2E2_523at 592.169 kb on + strand, within Pf6N2E2_523at 592.170 kb on - strand, within Pf6N2E2_523at 592.482 kb on - strand, within Pf6N2E2_523at 592.482 kb on - strand, within Pf6N2E2_523at 592.482 kb on - strand, within Pf6N2E2_523at 592.482 kb on - strand, within Pf6N2E2_523at 592.482 kb on - strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.595 kb on + strand, within Pf6N2E2_523at 592.664 kb on + strand, within Pf6N2E2_523at 592.664 kb on + strand, within Pf6N2E2_523at 592.664 kb on + strand, within Pf6N2E2_523at 592.664 kb on + strand, within Pf6N2E2_523at 592.664 kb on + strand, within Pf6N2E2_523at 592.665 kb on - strand, within Pf6N2E2_523at 592.665 kb on - strand, within Pf6N2E2_523at 592.665 kb on - strand, within Pf6N2E2_523at 592.665 kb on - strand, within Pf6N2E2_523at 592.665 kb on - strand, within Pf6N2E2_523at 592.665 kb on - strand, within Pf6N2E2_523

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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589,998 - Pf6N2E2_519 0.43 -0.0
590,121 - Pf6N2E2_519 0.55 -0.5
590,261 + Pf6N2E2_519 0.68 +2.1
590,262 - Pf6N2E2_519 0.68 +0.7
590,262 - Pf6N2E2_519 0.68 +1.3
590,262 - Pf6N2E2_519 0.68 +0.3
590,429 + Pf6N2E2_519 0.84 -0.1
590,430 - Pf6N2E2_519 0.84 -0.0
590,430 - Pf6N2E2_519 0.84 -0.7
590,430 - Pf6N2E2_519 0.84 -0.4
590,430 - Pf6N2E2_519 0.84 -0.2
590,430 - Pf6N2E2_519 0.84 -0.6
590,430 - Pf6N2E2_519 0.84 +0.1
590,724 + -0.3
590,724 + +0.1
590,724 + -0.0
590,724 + -0.4
590,725 - +0.5
590,729 - +0.5
590,763 + Pf6N2E2_521 0.18 +0.2
590,763 + Pf6N2E2_521 0.18 -0.3
590,763 + Pf6N2E2_521 0.18 -0.1
590,763 + Pf6N2E2_521 0.18 +0.1
590,764 - Pf6N2E2_521 0.18 +0.4
590,764 - Pf6N2E2_521 0.18 +0.8
590,907 + -1.0
591,060 + Pf6N2E2_522 0.13 +0.8
591,061 - Pf6N2E2_522 0.13 -0.1
591,061 - Pf6N2E2_522 0.13 -0.7
591,478 - Pf6N2E2_522 0.58 +0.8
591,658 + Pf6N2E2_522 0.77 -0.0
591,770 + Pf6N2E2_522 0.90 +0.8
591,770 + Pf6N2E2_522 0.90 +1.8
591,897 - +0.4
591,935 + -0.1
591,935 + -0.4
591,935 + +0.9
591,999 + +0.7
591,999 + -0.2
591,999 + -0.4
591,999 + +0.1
591,999 + -0.5
591,999 + -0.6
592,000 - -0.6
592,000 - -0.9
592,002 + -0.3
592,003 - +0.6
592,003 - -0.7
592,003 - -0.3
592,017 - -0.2
592,017 - -0.3
592,077 + -0.4
592,077 + -0.2
592,077 + +1.6
592,077 + -0.8
592,077 + +0.1
592,077 + -0.9
592,078 - +0.3
592,169 + Pf6N2E2_523 0.14 -0.1
592,169 + Pf6N2E2_523 0.14 +0.5
592,170 - Pf6N2E2_523 0.15 +3.1
592,482 - Pf6N2E2_523 0.35 +0.5
592,482 - Pf6N2E2_523 0.35 -1.4
592,482 - Pf6N2E2_523 0.35 -0.1
592,482 - Pf6N2E2_523 0.35 -1.5
592,482 - Pf6N2E2_523 0.35 +0.1
592,595 + Pf6N2E2_523 0.42 -0.9
592,595 + Pf6N2E2_523 0.42 +0.6
592,595 + Pf6N2E2_523 0.42 -0.1
592,595 + Pf6N2E2_523 0.42 +0.4
592,595 + Pf6N2E2_523 0.42 +0.8
592,595 + Pf6N2E2_523 0.42 -0.5
592,595 + Pf6N2E2_523 0.42 -0.9
592,595 + Pf6N2E2_523 0.42 +0.8
592,595 + Pf6N2E2_523 0.42 +0.3
592,595 + Pf6N2E2_523 0.42 +0.1
592,664 + Pf6N2E2_523 0.46 +0.1
592,664 + Pf6N2E2_523 0.46 +0.3
592,664 + Pf6N2E2_523 0.46 +0.2
592,664 + Pf6N2E2_523 0.46 +0.2
592,664 + Pf6N2E2_523 0.46 -0.8
592,665 - Pf6N2E2_523 0.46 -0.5
592,665 - Pf6N2E2_523 0.46 -0.0
592,665 - Pf6N2E2_523 0.46 -0.6
592,665 - Pf6N2E2_523 0.46 -0.2
592,665 - Pf6N2E2_523 0.46 +0.4
592,665 - Pf6N2E2_523 0.46 -0.4

Or see this region's nucleotide sequence