Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS06290 and RR41_RS06295 are separated by 133 nucleotides RR41_RS06295 and RR41_RS20020 overlap by 44 nucleotides RR41_RS20020 and RR41_RS06300 are separated by 355 nucleotides RR41_RS06300 and RR41_RS06305 are separated by 85 nucleotides
RR41_RS06290: RR41_RS06290 - RNA polymerase sigma factor RpoH, at 1,312,726 to 1,313,592
_RS06290
RR41_RS06295: RR41_RS06295 - sulfurtransferase TusA family protein, at 1,313,726 to 1,313,908
_RS06295
RR41_RS20020: RR41_RS20020 - hypothetical protein, at 1,313,865 to 1,313,990
_RS20020
RR41_RS06300: RR41_RS06300 - cold-shock protein, at 1,314,346 to 1,314,558
_RS06300
RR41_RS06305: RR41_RS06305 - ATP-dependent RNA helicase RhlB, at 1,314,644 to 1,315,813
_RS06305
Position (kb)
1314
1315 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1313.518 kb on - strand at 1313.606 kb on - strand at 1313.606 kb on - strand at 1313.606 kb on - strand at 1313.628 kb on - strand at 1313.657 kb on - strand at 1313.657 kb on - strand at 1313.657 kb on - strand at 1313.661 kb on - strand at 1313.677 kb on - strand at 1313.711 kb on + strand at 1313.785 kb on + strand, within RR41_RS06295 at 1313.856 kb on - strand, within RR41_RS06295 at 1313.856 kb on - strand, within RR41_RS06295 at 1313.897 kb on + strand at 1314.032 kb on + strand at 1314.033 kb on - strand at 1314.036 kb on + strand at 1314.036 kb on + strand at 1314.037 kb on - strand at 1314.037 kb on - strand at 1314.048 kb on - strand at 1314.072 kb on + strand at 1314.075 kb on + strand at 1314.112 kb on - strand at 1314.194 kb on + strand at 1314.194 kb on + strand at 1314.194 kb on + strand at 1314.195 kb on - strand at 1314.205 kb on + strand at 1314.236 kb on - strand at 1314.242 kb on - strand at 1314.253 kb on - strand at 1314.269 kb on + strand at 1314.345 kb on + strand at 1314.346 kb on - strand at 1314.369 kb on - strand, within RR41_RS06300 at 1314.371 kb on + strand, within RR41_RS06300 at 1314.431 kb on + strand, within RR41_RS06300 at 1314.431 kb on + strand, within RR41_RS06300 at 1314.431 kb on + strand, within RR41_RS06300 at 1314.456 kb on - strand, within RR41_RS06300 at 1314.477 kb on - strand, within RR41_RS06300 at 1314.502 kb on - strand, within RR41_RS06300 at 1314.509 kb on + strand, within RR41_RS06300 at 1314.509 kb on + strand, within RR41_RS06300 at 1314.509 kb on + strand, within RR41_RS06300 at 1314.509 kb on + strand, within RR41_RS06300 at 1314.509 kb on + strand, within RR41_RS06300 at 1314.556 kb on + strand at 1314.556 kb on + strand at 1314.615 kb on + strand at 1314.617 kb on + strand at 1314.618 kb on - strand at 1314.657 kb on + strand at 1314.703 kb on + strand at 1314.703 kb on + strand at 1314.768 kb on - strand, within RR41_RS06305 at 1314.770 kb on - strand, within RR41_RS06305 at 1314.817 kb on + strand, within RR41_RS06305 at 1314.818 kb on - strand, within RR41_RS06305 at 1314.843 kb on + strand, within RR41_RS06305 at 1314.912 kb on + strand, within RR41_RS06305 at 1314.912 kb on + strand, within RR41_RS06305 at 1314.988 kb on - strand, within RR41_RS06305 at 1314.988 kb on - strand, within RR41_RS06305 at 1314.999 kb on + strand, within RR41_RS06305 at 1315.000 kb on - strand, within RR41_RS06305 at 1315.080 kb on + strand, within RR41_RS06305 at 1315.210 kb on + strand, within RR41_RS06305 at 1315.254 kb on - strand, within RR41_RS06305 at 1315.352 kb on + strand, within RR41_RS06305 at 1315.353 kb on - strand, within RR41_RS06305 at 1315.376 kb on + strand, within RR41_RS06305 at 1315.376 kb on + strand, within RR41_RS06305 at 1315.377 kb on - strand, within RR41_RS06305 at 1315.463 kb on + strand, within RR41_RS06305 at 1315.503 kb on + strand, within RR41_RS06305 at 1315.503 kb on + strand, within RR41_RS06305 at 1315.503 kb on + strand, within RR41_RS06305 at 1315.525 kb on + strand, within RR41_RS06305
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 1,313,518 - -0.7 1,313,606 - +0.8 1,313,606 - -1.7 1,313,606 - +0.3 1,313,628 - -0.7 1,313,657 - +0.3 1,313,657 - +0.3 1,313,657 - -2.1 1,313,661 - -1.7 1,313,677 - +2.0 1,313,711 + -2.3 1,313,785 + RR41_RS06295 0.32 -0.7 1,313,856 - RR41_RS06295 0.71 +1.5 1,313,856 - RR41_RS06295 0.71 -1.1 1,313,897 + -1.7 1,314,032 + -0.5 1,314,033 - +0.3 1,314,036 + -0.7 1,314,036 + -2.1 1,314,037 - -1.3 1,314,037 - +0.5 1,314,048 - -0.6 1,314,072 + +0.3 1,314,075 + +0.7 1,314,112 - -0.7 1,314,194 + -2.3 1,314,194 + -0.7 1,314,194 + -0.7 1,314,195 - -1.3 1,314,205 + +1.3 1,314,236 - -0.9 1,314,242 - -2.1 1,314,253 - -0.7 1,314,269 + -0.7 1,314,345 + -1.7 1,314,346 - -0.7 1,314,369 - RR41_RS06300 0.11 -2.6 1,314,371 + RR41_RS06300 0.12 +0.3 1,314,431 + RR41_RS06300 0.40 -2.1 1,314,431 + RR41_RS06300 0.40 -2.1 1,314,431 + RR41_RS06300 0.40 -1.7 1,314,456 - RR41_RS06300 0.52 -0.7 1,314,477 - RR41_RS06300 0.62 -1.3 1,314,502 - RR41_RS06300 0.73 -0.7 1,314,509 + RR41_RS06300 0.77 -0.4 1,314,509 + RR41_RS06300 0.77 -0.7 1,314,509 + RR41_RS06300 0.77 -1.3 1,314,509 + RR41_RS06300 0.77 -2.3 1,314,509 + RR41_RS06300 0.77 -2.3 1,314,556 + -1.1 1,314,556 + +0.3 1,314,615 + +0.3 1,314,617 + +0.3 1,314,618 - -0.5 1,314,657 + -0.2 1,314,703 + -2.1 1,314,703 + -1.3 1,314,768 - RR41_RS06305 0.11 -0.6 1,314,770 - RR41_RS06305 0.11 +0.3 1,314,817 + RR41_RS06305 0.15 -1.7 1,314,818 - RR41_RS06305 0.15 -0.6 1,314,843 + RR41_RS06305 0.17 -3.1 1,314,912 + RR41_RS06305 0.23 +0.3 1,314,912 + RR41_RS06305 0.23 -0.7 1,314,988 - RR41_RS06305 0.29 -1.7 1,314,988 - RR41_RS06305 0.29 -3.2 1,314,999 + RR41_RS06305 0.30 -0.7 1,315,000 - RR41_RS06305 0.30 +2.8 1,315,080 + RR41_RS06305 0.37 -1.7 1,315,210 + RR41_RS06305 0.48 +0.3 1,315,254 - RR41_RS06305 0.52 -1.3 1,315,352 + RR41_RS06305 0.61 -0.7 1,315,353 - RR41_RS06305 0.61 +0.7 1,315,376 + RR41_RS06305 0.63 -1.0 1,315,376 + RR41_RS06305 0.63 +0.3 1,315,377 - RR41_RS06305 0.63 -1.3 1,315,463 + RR41_RS06305 0.70 +0.3 1,315,503 + RR41_RS06305 0.73 -2.3 1,315,503 + RR41_RS06305 0.73 -0.7 1,315,503 + RR41_RS06305 0.73 -2.3 1,315,525 + RR41_RS06305 0.75 +3.4
Or see this region's nucleotide sequence