Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00824 and MPMX20_00825 are separated by 40 nucleotides MPMX20_00825 and MPMX20_00826 are separated by 57 nucleotides MPMX20_00826 and MPMX20_00827 are separated by 11 nucleotides MPMX20_00827 and MPMX20_00828 are separated by 122 nucleotides
MPMX20_00824: MPMX20_00824 - hypothetical protein, at 866,762 to 868,009
_00824
MPMX20_00825: MPMX20_00825 - Aspartate 1-decarboxylase, at 868,050 to 868,430
_00825
MPMX20_00826: MPMX20_00826 - Pantothenate synthetase, at 868,488 to 869,339
_00826
MPMX20_00827: MPMX20_00827 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 869,351 to 870,142
_00827
MPMX20_00828: MPMX20_00828 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 870,265 to 870,744
_00828
Position (kb) 868
869
870 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 867.489 kb on + strand, within MPMX20_00824 at 867.625 kb on + strand, within MPMX20_00824 at 867.625 kb on + strand, within MPMX20_00824 at 867.652 kb on + strand, within MPMX20_00824 at 867.660 kb on - strand, within MPMX20_00824 at 867.660 kb on - strand, within MPMX20_00824 at 867.789 kb on + strand, within MPMX20_00824 at 868.005 kb on + strand at 868.010 kb on + strand at 868.013 kb on - strand at 868.018 kb on - strand at 868.021 kb on + strand at 868.024 kb on + strand at 868.031 kb on + strand at 868.031 kb on + strand at 868.031 kb on + strand at 868.039 kb on - strand at 868.046 kb on - strand at 868.264 kb on + strand, within MPMX20_00825 at 868.278 kb on - strand, within MPMX20_00825 at 868.381 kb on - strand, within MPMX20_00825 at 868.403 kb on + strand at 868.427 kb on + strand at 868.469 kb on + strand at 868.479 kb on + strand at 868.480 kb on + strand at 868.670 kb on + strand, within MPMX20_00826 at 868.678 kb on - strand, within MPMX20_00826 at 868.714 kb on + strand, within MPMX20_00826 at 868.804 kb on + strand, within MPMX20_00826 at 868.906 kb on + strand, within MPMX20_00826 at 868.921 kb on + strand, within MPMX20_00826 at 868.921 kb on + strand, within MPMX20_00826 at 868.929 kb on - strand, within MPMX20_00826 at 869.001 kb on - strand, within MPMX20_00826 at 869.017 kb on - strand, within MPMX20_00826 at 869.027 kb on + strand, within MPMX20_00826 at 869.184 kb on - strand, within MPMX20_00826 at 869.250 kb on - strand, within MPMX20_00826 at 869.339 kb on - strand at 869.390 kb on - strand at 869.400 kb on + strand at 869.408 kb on + strand at 869.408 kb on - strand at 869.421 kb on + strand at 869.513 kb on - strand, within MPMX20_00827 at 869.521 kb on + strand, within MPMX20_00827 at 869.563 kb on + strand, within MPMX20_00827 at 869.596 kb on + strand, within MPMX20_00827 at 869.684 kb on - strand, within MPMX20_00827 at 869.689 kb on + strand, within MPMX20_00827 at 869.697 kb on - strand, within MPMX20_00827 at 869.729 kb on + strand, within MPMX20_00827 at 869.742 kb on - strand, within MPMX20_00827 at 869.858 kb on - strand, within MPMX20_00827 at 869.917 kb on - strand, within MPMX20_00827 at 870.027 kb on + strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.033 kb on - strand, within MPMX20_00827 at 870.035 kb on - strand, within MPMX20_00827 at 870.035 kb on - strand, within MPMX20_00827 at 870.067 kb on - strand at 870.067 kb on - strand at 870.081 kb on - strand at 870.093 kb on + strand at 870.130 kb on - strand at 870.192 kb on + strand at 870.200 kb on - strand at 870.250 kb on - strand at 870.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 867,489 + MPMX20_00824 0.58 +0.8 867,625 + MPMX20_00824 0.69 +0.2 867,625 + MPMX20_00824 0.69 -0.6 867,652 + MPMX20_00824 0.71 -0.3 867,660 - MPMX20_00824 0.72 +0.8 867,660 - MPMX20_00824 0.72 +0.3 867,789 + MPMX20_00824 0.82 -0.9 868,005 + -2.1 868,010 + -0.1 868,013 - +0.1 868,018 - +1.7 868,021 + +0.5 868,024 + +0.7 868,031 + -0.1 868,031 + +0.5 868,031 + +1.0 868,039 - -0.1 868,046 - +1.0 868,264 + MPMX20_00825 0.56 +0.5 868,278 - MPMX20_00825 0.60 -0.6 868,381 - MPMX20_00825 0.87 -1.0 868,403 + -0.4 868,427 + -0.6 868,469 + -0.1 868,479 + +0.7 868,480 + +0.2 868,670 + MPMX20_00826 0.21 -0.0 868,678 - MPMX20_00826 0.22 -0.1 868,714 + MPMX20_00826 0.27 -0.2 868,804 + MPMX20_00826 0.37 +0.4 868,906 + MPMX20_00826 0.49 -2.2 868,921 + MPMX20_00826 0.51 -0.6 868,921 + MPMX20_00826 0.51 -2.7 868,929 - MPMX20_00826 0.52 -2.2 869,001 - MPMX20_00826 0.60 -0.6 869,017 - MPMX20_00826 0.62 -1.2 869,027 + MPMX20_00826 0.63 +0.5 869,184 - MPMX20_00826 0.82 -0.8 869,250 - MPMX20_00826 0.89 -0.9 869,339 - -3.2 869,390 - -0.3 869,400 + +0.4 869,408 + -0.2 869,408 - -0.3 869,421 + -0.2 869,513 - MPMX20_00827 0.20 -0.1 869,521 + MPMX20_00827 0.21 -1.1 869,563 + MPMX20_00827 0.27 -0.2 869,596 + MPMX20_00827 0.31 -0.3 869,684 - MPMX20_00827 0.42 -0.4 869,689 + MPMX20_00827 0.43 -1.3 869,697 - MPMX20_00827 0.44 +0.4 869,729 + MPMX20_00827 0.48 +0.3 869,742 - MPMX20_00827 0.49 -1.1 869,858 - MPMX20_00827 0.64 -1.5 869,917 - MPMX20_00827 0.71 +0.1 870,027 + MPMX20_00827 0.85 -0.1 870,027 - MPMX20_00827 0.85 -0.7 870,027 - MPMX20_00827 0.85 -0.2 870,027 - MPMX20_00827 0.85 -0.3 870,027 - MPMX20_00827 0.85 -0.9 870,033 - MPMX20_00827 0.86 -0.3 870,035 - MPMX20_00827 0.86 -0.7 870,035 - MPMX20_00827 0.86 -0.3 870,067 - -1.1 870,067 - -0.8 870,081 - -2.8 870,093 + +2.5 870,130 - -0.2 870,192 + +0.2 870,200 - +0.3 870,250 - -0.0 870,250 - -3.0
Or see this region's nucleotide sequence