Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4420 and GFF4421 are separated by 158 nucleotides GFF4421 and GFF4422 are separated by 5 nucleotides GFF4422 and GFF4423 are separated by 19 nucleotides GFF4423 and GFF4424 are separated by 142 nucleotides
GFF4420 - hypothetical protein, at 252 to 983
GFF4420
GFF4421 - Corrinoid adenosyltransferase, at 1,142 to 1,738
GFF4421
GFF4422 - hypothetical protein, at 1,744 to 1,938
GFF4422
GFF4423 - Tryptophan-rich protein TspO, at 1,958 to 2,464
GFF4423
GFF4424 - hypothetical protein, at 2,607 to 3,053
GFF4424
Position (kb) 1
2 Strain fitness (log2 ratio) -2
-1
0
1
2 at 0.231 kb on + strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.705 kb on + strand, within GFF4420 at 0.748 kb on + strand, within GFF4420 at 0.749 kb on - strand, within GFF4420 at 0.784 kb on + strand, within GFF4420 at 0.785 kb on - strand, within GFF4420 at 1.043 kb on + strand at 1.044 kb on - strand at 1.044 kb on - strand at 1.106 kb on + strand at 1.106 kb on + strand at 1.107 kb on - strand at 1.242 kb on + strand, within GFF4421 at 1.243 kb on - strand, within GFF4421 at 1.635 kb on + strand, within GFF4421 at 1.636 kb on - strand, within GFF4421 at 1.636 kb on - strand, within GFF4421 at 1.717 kb on - strand at 1.717 kb on - strand at 1.998 kb on + strand at 1.998 kb on + strand at 1.999 kb on - strand at 2.017 kb on - strand, within GFF4423 at 2.263 kb on - strand, within GFF4423
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 231 + +1.8 232 - +0.6 232 - -0.4 232 - -0.7 232 - +0.5 232 - -1.2 705 + GFF4420 0.62 +0.3 748 + GFF4420 0.68 -0.9 749 - GFF4420 0.68 -0.1 784 + GFF4420 0.73 -0.2 785 - GFF4420 0.73 -0.9 1,043 + -0.1 1,044 - +0.7 1,044 - +0.9 1,106 + -0.8 1,106 + -0.1 1,107 - +0.1 1,242 + GFF4421 0.17 -0.6 1,243 - GFF4421 0.17 -0.4 1,635 + GFF4421 0.83 -0.7 1,636 - GFF4421 0.83 +1.5 1,636 - GFF4421 0.83 -1.3 1,717 - +1.3 1,717 - +0.4 1,998 + -0.4 1,998 + -2.0 1,999 - +0.2 2,017 - GFF4423 0.12 +0.9 2,263 - GFF4423 0.60 +0.8
Or see this region's nucleotide sequence