Experiment: Mixed culture with Prochlorococcus marinus str. MED4; Experiment C, time point 7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01148 and MIT1002_01149 are separated by 155 nucleotides MIT1002_01149 and MIT1002_01150 are separated by 45 nucleotides MIT1002_01150 and MIT1002_01151 are separated by 308 nucleotides
MIT1002_01148: MIT1002_01148 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 1,260,669 to 1,261,679
_01148
MIT1002_01149: MIT1002_01149 - Maleylpyruvate isomerase, at 1,261,835 to 1,262,488
_01149
MIT1002_01150: MIT1002_01150 - Leucine-responsive regulatory protein, at 1,262,534 to 1,263,001
_01150
MIT1002_01151: MIT1002_01151 - Leucine dehydrogenase, at 1,263,310 to 1,264,368
_01151
Position (kb)
1262
1263
1264 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1261.536 kb on - strand, within MIT1002_01148 at 1261.544 kb on + strand, within MIT1002_01148 at 1261.554 kb on + strand, within MIT1002_01148 at 1261.562 kb on + strand, within MIT1002_01148 at 1261.570 kb on - strand, within MIT1002_01148 at 1261.578 kb on - strand, within MIT1002_01148 at 1261.656 kb on - strand at 1261.782 kb on - strand at 1261.791 kb on + strand at 1261.792 kb on - strand at 1261.792 kb on - strand at 1261.799 kb on - strand at 1261.882 kb on + strand at 1261.908 kb on + strand, within MIT1002_01149 at 1261.925 kb on + strand, within MIT1002_01149 at 1261.945 kb on + strand, within MIT1002_01149 at 1261.968 kb on - strand, within MIT1002_01149 at 1261.981 kb on + strand, within MIT1002_01149 at 1261.981 kb on + strand, within MIT1002_01149 at 1261.981 kb on + strand, within MIT1002_01149 at 1261.981 kb on + strand, within MIT1002_01149 at 1261.989 kb on - strand, within MIT1002_01149 at 1262.041 kb on - strand, within MIT1002_01149 at 1262.077 kb on + strand, within MIT1002_01149 at 1262.261 kb on - strand, within MIT1002_01149 at 1262.261 kb on - strand, within MIT1002_01149 at 1262.261 kb on - strand, within MIT1002_01149 at 1262.264 kb on + strand, within MIT1002_01149 at 1262.266 kb on - strand, within MIT1002_01149 at 1262.333 kb on - strand, within MIT1002_01149 at 1262.370 kb on - strand, within MIT1002_01149 at 1262.410 kb on - strand, within MIT1002_01149 at 1262.418 kb on - strand, within MIT1002_01149 at 1262.444 kb on - strand at 1262.455 kb on + strand at 1262.463 kb on - strand at 1262.490 kb on + strand at 1262.508 kb on + strand at 1262.509 kb on - strand at 1262.583 kb on - strand, within MIT1002_01150 at 1262.653 kb on + strand, within MIT1002_01150 at 1262.653 kb on + strand, within MIT1002_01150 at 1262.653 kb on + strand, within MIT1002_01150 at 1262.653 kb on + strand, within MIT1002_01150 at 1262.661 kb on - strand, within MIT1002_01150 at 1262.661 kb on - strand, within MIT1002_01150 at 1262.661 kb on - strand, within MIT1002_01150 at 1262.706 kb on + strand, within MIT1002_01150 at 1262.714 kb on - strand, within MIT1002_01150 at 1262.795 kb on + strand, within MIT1002_01150 at 1262.809 kb on - strand, within MIT1002_01150 at 1262.894 kb on + strand, within MIT1002_01150 at 1262.901 kb on + strand, within MIT1002_01150 at 1262.923 kb on + strand, within MIT1002_01150 at 1263.139 kb on - strand at 1263.146 kb on - strand at 1263.187 kb on + strand at 1263.195 kb on - strand at 1263.203 kb on - strand at 1263.286 kb on - strand at 1263.407 kb on + strand at 1263.415 kb on - strand at 1263.427 kb on + strand, within MIT1002_01151 at 1263.468 kb on + strand, within MIT1002_01151 at 1263.477 kb on - strand, within MIT1002_01151 at 1263.492 kb on + strand, within MIT1002_01151 at 1263.500 kb on - strand, within MIT1002_01151 at 1263.636 kb on + strand, within MIT1002_01151 at 1263.644 kb on - strand, within MIT1002_01151 at 1263.646 kb on + strand, within MIT1002_01151 at 1263.699 kb on + strand, within MIT1002_01151 at 1263.788 kb on - strand, within MIT1002_01151 at 1263.816 kb on - strand, within MIT1002_01151 at 1263.816 kb on - strand, within MIT1002_01151 at 1263.816 kb on - strand, within MIT1002_01151 at 1263.838 kb on - strand, within MIT1002_01151 at 1263.838 kb on - strand, within MIT1002_01151 at 1263.838 kb on - strand, within MIT1002_01151 at 1263.853 kb on + strand, within MIT1002_01151 at 1263.860 kb on + strand, within MIT1002_01151 at 1263.868 kb on - strand, within MIT1002_01151 at 1263.891 kb on + strand, within MIT1002_01151 at 1263.908 kb on + strand, within MIT1002_01151 at 1263.915 kb on + strand, within MIT1002_01151 at 1263.915 kb on + strand, within MIT1002_01151 at 1263.915 kb on + strand, within MIT1002_01151 at 1263.916 kb on - strand, within MIT1002_01151 at 1263.923 kb on - strand, within MIT1002_01151 at 1263.980 kb on + strand, within MIT1002_01151
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture with Prochlorococcus marinus str. MED4; Experiment C, time point 7 remove 1,261,536 - MIT1002_01148 0.86 -1.2 1,261,544 + MIT1002_01148 0.87 -0.9 1,261,554 + MIT1002_01148 0.88 -1.7 1,261,562 + MIT1002_01148 0.88 -1.9 1,261,570 - MIT1002_01148 0.89 -1.6 1,261,578 - MIT1002_01148 0.90 -0.7 1,261,656 - -0.2 1,261,782 - +0.7 1,261,791 + -0.4 1,261,792 - +4.0 1,261,792 - -1.0 1,261,799 - -0.4 1,261,882 + -0.7 1,261,908 + MIT1002_01149 0.11 +0.2 1,261,925 + MIT1002_01149 0.14 +0.9 1,261,945 + MIT1002_01149 0.17 -1.7 1,261,968 - MIT1002_01149 0.20 -0.3 1,261,981 + MIT1002_01149 0.22 -0.5 1,261,981 + MIT1002_01149 0.22 +0.0 1,261,981 + MIT1002_01149 0.22 +2.1 1,261,981 + MIT1002_01149 0.22 +1.4 1,261,989 - MIT1002_01149 0.24 -0.4 1,262,041 - MIT1002_01149 0.31 -2.1 1,262,077 + MIT1002_01149 0.37 -0.5 1,262,261 - MIT1002_01149 0.65 +0.6 1,262,261 - MIT1002_01149 0.65 +0.2 1,262,261 - MIT1002_01149 0.65 +1.6 1,262,264 + MIT1002_01149 0.66 +0.6 1,262,266 - MIT1002_01149 0.66 -0.5 1,262,333 - MIT1002_01149 0.76 +2.3 1,262,370 - MIT1002_01149 0.82 +0.4 1,262,410 - MIT1002_01149 0.88 -0.2 1,262,418 - MIT1002_01149 0.89 +1.7 1,262,444 - -0.2 1,262,455 + +0.0 1,262,463 - +0.2 1,262,490 + +1.4 1,262,508 + -0.4 1,262,509 - -0.7 1,262,583 - MIT1002_01150 0.10 -0.6 1,262,653 + MIT1002_01150 0.25 -0.5 1,262,653 + MIT1002_01150 0.25 -3.8 1,262,653 + MIT1002_01150 0.25 -1.3 1,262,653 + MIT1002_01150 0.25 -1.2 1,262,661 - MIT1002_01150 0.27 -1.1 1,262,661 - MIT1002_01150 0.27 -4.9 1,262,661 - MIT1002_01150 0.27 +1.6 1,262,706 + MIT1002_01150 0.37 -3.6 1,262,714 - MIT1002_01150 0.38 +0.3 1,262,795 + MIT1002_01150 0.56 -1.7 1,262,809 - MIT1002_01150 0.59 -0.3 1,262,894 + MIT1002_01150 0.77 +1.4 1,262,901 + MIT1002_01150 0.78 -3.4 1,262,923 + MIT1002_01150 0.83 -1.6 1,263,139 - -0.5 1,263,146 - -0.3 1,263,187 + -0.1 1,263,195 - -2.4 1,263,203 - -1.7 1,263,286 - +0.4 1,263,407 + -1.5 1,263,415 - -3.4 1,263,427 + MIT1002_01151 0.11 +0.3 1,263,468 + MIT1002_01151 0.15 -1.9 1,263,477 - MIT1002_01151 0.16 -0.4 1,263,492 + MIT1002_01151 0.17 -0.7 1,263,500 - MIT1002_01151 0.18 -0.1 1,263,636 + MIT1002_01151 0.31 -0.8 1,263,644 - MIT1002_01151 0.32 -1.7 1,263,646 + MIT1002_01151 0.32 +0.9 1,263,699 + MIT1002_01151 0.37 -0.4 1,263,788 - MIT1002_01151 0.45 -0.8 1,263,816 - MIT1002_01151 0.48 -1.3 1,263,816 - MIT1002_01151 0.48 -2.1 1,263,816 - MIT1002_01151 0.48 -0.4 1,263,838 - MIT1002_01151 0.50 -1.6 1,263,838 - MIT1002_01151 0.50 +3.1 1,263,838 - MIT1002_01151 0.50 -1.5 1,263,853 + MIT1002_01151 0.51 -0.2 1,263,860 + MIT1002_01151 0.52 -1.0 1,263,868 - MIT1002_01151 0.53 +0.0 1,263,891 + MIT1002_01151 0.55 -1.3 1,263,908 + MIT1002_01151 0.56 -2.2 1,263,915 + MIT1002_01151 0.57 -0.7 1,263,915 + MIT1002_01151 0.57 -0.2 1,263,915 + MIT1002_01151 0.57 -1.5 1,263,916 - MIT1002_01151 0.57 -0.3 1,263,923 - MIT1002_01151 0.58 -2.4 1,263,980 + MIT1002_01151 0.63 -1.9
Or see this region's nucleotide sequence