Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7033 and GFF7034 overlap by 4 nucleotides GFF7034 and GFF7035 are separated by 143 nucleotides
GFF7033 - protein of unknown function DUF427, at 207 to 491
GFF7033
GFF7034 - FIG00538743: hypothetical protein, at 488 to 1,243
GFF7034
GFF7035 - UspA, at 1,387 to 1,827
GFF7035
Position (kb) 0
1 Strain fitness (log2 ratio) -2
-1
0
1 at 0.098 kb on - strand at 0.098 kb on - strand at 0.102 kb on - strand at 0.102 kb on - strand at 0.102 kb on - strand at 0.143 kb on - strand at 0.276 kb on + strand, within GFF7033 at 0.276 kb on + strand, within GFF7033 at 0.360 kb on + strand, within GFF7033 at 0.360 kb on + strand, within GFF7033 at 0.360 kb on + strand, within GFF7033 at 0.360 kb on + strand, within GFF7033 at 0.361 kb on - strand, within GFF7033 at 0.363 kb on + strand, within GFF7033 at 0.363 kb on + strand, within GFF7033 at 0.363 kb on + strand, within GFF7033 at 0.363 kb on + strand, within GFF7033 at 0.363 kb on + strand, within GFF7033 at 0.364 kb on - strand, within GFF7033 at 0.364 kb on - strand, within GFF7033 at 0.364 kb on - strand, within GFF7033 at 0.408 kb on + strand, within GFF7033 at 0.408 kb on + strand, within GFF7033 at 0.409 kb on - strand, within GFF7033 at 0.409 kb on - strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.411 kb on + strand, within GFF7033 at 0.412 kb on - strand, within GFF7033 at 0.412 kb on - strand, within GFF7033 at 0.412 kb on - strand, within GFF7033 at 0.412 kb on - strand, within GFF7033 at 0.412 kb on - strand, within GFF7033 at 0.412 kb on - strand, within GFF7033 at 0.560 kb on + strand at 0.560 kb on + strand at 0.561 kb on - strand at 0.561 kb on - strand at 0.561 kb on - strand at 0.779 kb on + strand, within GFF7034 at 0.779 kb on + strand, within GFF7034 at 0.779 kb on + strand, within GFF7034 at 0.780 kb on - strand, within GFF7034 at 0.780 kb on - strand, within GFF7034 at 0.780 kb on - strand, within GFF7034 at 0.780 kb on - strand, within GFF7034 at 0.917 kb on + strand, within GFF7034 at 0.917 kb on + strand, within GFF7034 at 0.917 kb on + strand, within GFF7034 at 0.918 kb on - strand, within GFF7034 at 0.918 kb on - strand, within GFF7034 at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.297 kb on + strand at 1.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 98 - -0.0 98 - -0.1 102 - -0.8 102 - -1.0 102 - -1.0 143 - +0.1 276 + GFF7033 0.24 -0.4 276 + GFF7033 0.24 +0.3 360 + GFF7033 0.54 +0.3 360 + GFF7033 0.54 +0.3 360 + GFF7033 0.54 +0.2 360 + GFF7033 0.54 -1.3 361 - GFF7033 0.54 -0.1 363 + GFF7033 0.55 -0.3 363 + GFF7033 0.55 +0.3 363 + GFF7033 0.55 +1.2 363 + GFF7033 0.55 -1.1 363 + GFF7033 0.55 +0.5 364 - GFF7033 0.55 -0.7 364 - GFF7033 0.55 -0.1 364 - GFF7033 0.55 -0.8 408 + GFF7033 0.71 -0.0 408 + GFF7033 0.71 -0.6 409 - GFF7033 0.71 +0.6 409 - GFF7033 0.71 +0.9 411 + GFF7033 0.72 -2.3 411 + GFF7033 0.72 +0.9 411 + GFF7033 0.72 +0.5 411 + GFF7033 0.72 +0.2 411 + GFF7033 0.72 +0.8 411 + GFF7033 0.72 -0.0 411 + GFF7033 0.72 +0.0 411 + GFF7033 0.72 -0.2 412 - GFF7033 0.72 +0.6 412 - GFF7033 0.72 -0.3 412 - GFF7033 0.72 -0.2 412 - GFF7033 0.72 -0.7 412 - GFF7033 0.72 -1.1 412 - GFF7033 0.72 +0.5 560 + +0.2 560 + -0.3 561 - +0.1 561 - +0.3 561 - -1.1 779 + GFF7034 0.38 -0.0 779 + GFF7034 0.38 -0.8 779 + GFF7034 0.38 +0.1 780 - GFF7034 0.39 -0.1 780 - GFF7034 0.39 +0.3 780 - GFF7034 0.39 -1.4 780 - GFF7034 0.39 -0.2 917 + GFF7034 0.57 -1.8 917 + GFF7034 0.57 -1.9 917 + GFF7034 0.57 +1.0 918 - GFF7034 0.57 -0.4 918 - GFF7034 0.57 -1.7 1,297 + -0.6 1,297 + -0.3 1,297 + +0.3 1,297 + +0.4 1,297 + +0.7 1,297 + -0.0 1,297 + -0.6 1,297 + +0.1 1,297 + +0.8 1,297 + -1.4 1,298 - +1.3
Or see this region's nucleotide sequence