Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1348 and GFF1349 are separated by 9 nucleotides GFF1349 and GFF1350 are separated by 24 nucleotides GFF1350 and GFF1351 are separated by 98 nucleotides
GFF1348 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 691,275 to 692,072
GFF1348
GFF1349 - Thioesterase, at 692,082 to 692,546
GFF1349
GFF1350 - Quinone oxidoreductase (EC 1.6.5.5), at 692,571 to 693,587
GFF1350
GFF1351 - Acyl-CoA dehydrogenase, at 693,686 to 695,326
GFF1351
Position (kb)
692
693
694 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 691.650 kb on + strand, within GFF1348 at 691.684 kb on - strand, within GFF1348 at 691.773 kb on + strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 692.199 kb on + strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.431 kb on - strand, within GFF1349 at 692.431 kb on - strand, within GFF1349 at 692.478 kb on + strand, within GFF1349 at 692.566 kb on + strand at 692.567 kb on - strand at 692.567 kb on - strand at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.697 kb on + strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.698 kb on - strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.715 kb on + strand, within GFF1350 at 692.716 kb on - strand, within GFF1350 at 692.716 kb on - strand, within GFF1350 at 692.748 kb on + strand, within GFF1350 at 692.748 kb on + strand, within GFF1350 at 692.749 kb on - strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.826 kb on + strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.827 kb on - strand, within GFF1350 at 692.871 kb on + strand, within GFF1350 at 692.871 kb on + strand, within GFF1350 at 692.872 kb on - strand, within GFF1350 at 692.872 kb on - strand, within GFF1350 at 692.983 kb on - strand, within GFF1350 at 693.132 kb on + strand, within GFF1350 at 693.132 kb on + strand, within GFF1350 at 693.133 kb on - strand, within GFF1350 at 693.133 kb on - strand, within GFF1350 at 693.133 kb on - strand, within GFF1350 at 693.133 kb on - strand, within GFF1350 at 693.264 kb on + strand, within GFF1350 at 693.264 kb on + strand, within GFF1350 at 693.265 kb on - strand, within GFF1350 at 693.265 kb on - strand, within GFF1350 at 693.265 kb on - strand, within GFF1350 at 693.509 kb on + strand at 693.679 kb on + strand at 693.679 kb on + strand at 693.734 kb on + strand at 693.734 kb on + strand at 693.734 kb on + strand at 693.734 kb on + strand at 693.734 kb on + strand at 693.735 kb on - strand at 693.735 kb on - strand at 693.944 kb on + strand, within GFF1351 at 693.944 kb on + strand, within GFF1351 at 693.945 kb on - strand, within GFF1351 at 693.945 kb on - strand, within GFF1351 at 694.094 kb on + strand, within GFF1351 at 694.094 kb on + strand, within GFF1351 at 694.095 kb on - strand, within GFF1351 at 694.095 kb on - strand, within GFF1351 at 694.134 kb on - strand, within GFF1351 at 694.134 kb on - strand, within GFF1351 at 694.310 kb on + strand, within GFF1351
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 691,650 + GFF1348 0.47 +0.1 691,684 - GFF1348 0.51 +0.1 691,773 + GFF1348 0.62 -0.4 691,774 - GFF1348 0.63 +1.7 691,774 - GFF1348 0.63 -0.0 691,836 + GFF1348 0.70 -0.7 691,836 + GFF1348 0.70 +0.2 691,836 + GFF1348 0.70 -1.1 691,837 - GFF1348 0.70 -1.5 691,837 - GFF1348 0.70 -0.5 691,837 - GFF1348 0.70 -0.6 691,837 - GFF1348 0.70 +0.6 692,199 + GFF1349 0.25 -1.6 692,200 - GFF1349 0.25 -0.4 692,200 - GFF1349 0.25 -0.1 692,431 - GFF1349 0.75 -0.2 692,431 - GFF1349 0.75 -0.4 692,478 + GFF1349 0.85 +0.1 692,566 + -0.4 692,567 - -1.5 692,567 - -0.4 692,697 + GFF1350 0.12 -0.6 692,697 + GFF1350 0.12 +0.2 692,697 + GFF1350 0.12 +0.9 692,697 + GFF1350 0.12 +0.9 692,697 + GFF1350 0.12 -0.7 692,697 + GFF1350 0.12 +0.5 692,698 - GFF1350 0.12 -0.1 692,698 - GFF1350 0.12 -0.1 692,698 - GFF1350 0.12 -2.7 692,698 - GFF1350 0.12 +0.1 692,698 - GFF1350 0.12 +1.4 692,698 - GFF1350 0.12 -0.4 692,698 - GFF1350 0.12 -0.6 692,698 - GFF1350 0.12 +0.4 692,698 - GFF1350 0.12 -0.9 692,715 + GFF1350 0.14 +0.2 692,715 + GFF1350 0.14 -0.3 692,715 + GFF1350 0.14 +0.1 692,716 - GFF1350 0.14 -0.5 692,716 - GFF1350 0.14 -0.2 692,748 + GFF1350 0.17 -0.1 692,748 + GFF1350 0.17 -1.4 692,749 - GFF1350 0.18 +2.2 692,826 + GFF1350 0.25 -0.4 692,826 + GFF1350 0.25 -0.3 692,826 + GFF1350 0.25 -0.5 692,827 - GFF1350 0.25 +0.2 692,827 - GFF1350 0.25 -0.5 692,827 - GFF1350 0.25 -0.4 692,827 - GFF1350 0.25 -0.2 692,827 - GFF1350 0.25 +0.6 692,871 + GFF1350 0.29 +0.3 692,871 + GFF1350 0.29 -0.5 692,872 - GFF1350 0.30 +0.1 692,872 - GFF1350 0.30 +0.5 692,983 - GFF1350 0.41 +0.2 693,132 + GFF1350 0.55 +1.1 693,132 + GFF1350 0.55 -1.1 693,133 - GFF1350 0.55 +1.1 693,133 - GFF1350 0.55 +2.7 693,133 - GFF1350 0.55 -1.9 693,133 - GFF1350 0.55 +0.2 693,264 + GFF1350 0.68 -1.9 693,264 + GFF1350 0.68 +0.4 693,265 - GFF1350 0.68 +0.2 693,265 - GFF1350 0.68 -0.9 693,265 - GFF1350 0.68 +0.8 693,509 + +0.7 693,679 + +0.1 693,679 + +1.6 693,734 + -0.1 693,734 + -0.6 693,734 + +1.4 693,734 + -2.4 693,734 + +0.5 693,735 - +0.0 693,735 - -1.1 693,944 + GFF1351 0.16 +1.5 693,944 + GFF1351 0.16 -0.2 693,945 - GFF1351 0.16 -0.9 693,945 - GFF1351 0.16 -1.8 694,094 + GFF1351 0.25 -1.9 694,094 + GFF1351 0.25 -0.7 694,095 - GFF1351 0.25 -0.7 694,095 - GFF1351 0.25 +0.4 694,134 - GFF1351 0.27 +0.5 694,134 - GFF1351 0.27 -0.8 694,310 + GFF1351 0.38 -0.4
Or see this region's nucleotide sequence