Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3425 and GFF3426 overlap by 4 nucleotides GFF3426 and GFF3427 overlap by 4 nucleotides GFF3427 and GFF3428 are separated by 4 nucleotides GFF3428 and GFF3429 are separated by 16 nucleotides
GFF3425 - no description, at 249,814 to 250,161
GFF3425
GFF3426 - Predicted kinase, at 250,158 to 250,973
GFF3426
GFF3427 - FIG01006093: hypothetical protein, at 250,970 to 251,479
GFF3427
GFF3428 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 251,484 to 252,296
GFF3428
GFF3429 - Transcriptional regulator, AraC family, at 252,313 to 253,233
GFF3429
Position (kb)
250
251
252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 250.057 kb on + strand, within GFF3425 at 250.057 kb on + strand, within GFF3425 at 250.058 kb on - strand, within GFF3425 at 250.269 kb on + strand, within GFF3426 at 250.269 kb on + strand, within GFF3426 at 250.569 kb on + strand, within GFF3426 at 250.569 kb on + strand, within GFF3426 at 251.282 kb on + strand, within GFF3427 at 251.283 kb on - strand, within GFF3427 at 251.283 kb on - strand, within GFF3427 at 251.436 kb on + strand at 251.444 kb on + strand at 251.445 kb on - strand at 251.445 kb on - strand at 251.511 kb on + strand at 251.512 kb on - strand at 251.637 kb on + strand, within GFF3428 at 251.676 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.686 kb on - strand, within GFF3428 at 251.686 kb on - strand, within GFF3428 at 251.686 kb on - strand, within GFF3428 at 252.060 kb on + strand, within GFF3428 at 252.294 kb on + strand at 252.305 kb on + strand at 252.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 250,057 + GFF3425 0.70 -0.3 250,057 + GFF3425 0.70 -0.2 250,058 - GFF3425 0.70 -0.1 250,269 + GFF3426 0.14 -0.4 250,269 + GFF3426 0.14 -0.4 250,569 + GFF3426 0.50 -0.4 250,569 + GFF3426 0.50 +0.2 251,282 + GFF3427 0.61 +0.3 251,283 - GFF3427 0.61 -1.3 251,283 - GFF3427 0.61 -1.3 251,436 + -0.6 251,444 + +0.1 251,445 - +1.1 251,445 - +0.1 251,511 + -0.5 251,512 - +1.0 251,637 + GFF3428 0.19 +1.1 251,676 + GFF3428 0.24 -0.3 251,685 + GFF3428 0.25 +0.0 251,685 + GFF3428 0.25 +1.2 251,685 + GFF3428 0.25 -0.5 251,685 + GFF3428 0.25 +0.9 251,685 + GFF3428 0.25 -0.4 251,685 + GFF3428 0.25 -0.4 251,685 + GFF3428 0.25 -0.5 251,685 + GFF3428 0.25 +0.7 251,685 + GFF3428 0.25 +0.5 251,686 - GFF3428 0.25 -0.3 251,686 - GFF3428 0.25 -0.4 251,686 - GFF3428 0.25 -1.8 252,060 + GFF3428 0.71 +1.9 252,294 + +0.7 252,305 + -0.0 252,305 + +0.7
Or see this region's nucleotide sequence