Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6336 and GFF6337 are separated by 139 nucleotides GFF6337 and GFF6338 overlap by 4 nucleotides GFF6338 and GFF6339 are separated by 425 nucleotides GFF6339 and GFF6340 are separated by 232 nucleotides
GFF6336 - no description, at 1,411 to 1,911
GFF6336
GFF6337 - Ser/Thr protein phosphatase family protein, at 2,051 to 2,884
GFF6337
GFF6338 - no description, at 2,881 to 3,012
GFF6338
GFF6339 - no description, at 3,438 to 3,563
GFF6339
GFF6340 - no description, at 3,796 to 3,966
GFF6340
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.005 kb on + strand at 2.005 kb on + strand at 2.006 kb on - strand at 2.015 kb on + strand at 2.015 kb on + strand at 2.015 kb on + strand at 2.046 kb on + strand at 2.046 kb on + strand at 2.060 kb on + strand at 2.061 kb on - strand at 2.061 kb on - strand at 2.061 kb on - strand at 2.061 kb on - strand at 2.115 kb on + strand at 2.116 kb on - strand at 2.176 kb on + strand, within GFF6337 at 2.176 kb on + strand, within GFF6337 at 2.177 kb on - strand, within GFF6337 at 2.177 kb on - strand, within GFF6337 at 2.177 kb on - strand, within GFF6337 at 2.177 kb on - strand, within GFF6337 at 2.341 kb on + strand, within GFF6337 at 2.341 kb on + strand, within GFF6337 at 2.342 kb on - strand, within GFF6337 at 2.342 kb on - strand, within GFF6337 at 2.374 kb on + strand, within GFF6337 at 2.375 kb on - strand, within GFF6337 at 2.375 kb on - strand, within GFF6337 at 2.389 kb on + strand, within GFF6337 at 2.389 kb on + strand, within GFF6337 at 2.389 kb on + strand, within GFF6337 at 2.389 kb on + strand, within GFF6337 at 2.389 kb on + strand, within GFF6337 at 2.390 kb on - strand, within GFF6337 at 2.390 kb on - strand, within GFF6337 at 2.390 kb on - strand, within GFF6337 at 2.390 kb on - strand, within GFF6337 at 2.390 kb on - strand, within GFF6337 at 2.390 kb on - strand, within GFF6337 at 2.409 kb on - strand, within GFF6337 at 2.409 kb on - strand, within GFF6337 at 2.467 kb on - strand, within GFF6337 at 2.688 kb on + strand, within GFF6337 at 2.689 kb on - strand, within GFF6337 at 2.689 kb on - strand, within GFF6337 at 2.814 kb on + strand at 2.814 kb on + strand at 2.983 kb on + strand, within GFF6338 at 2.983 kb on + strand, within GFF6338 at 2.983 kb on + strand, within GFF6338 at 2.983 kb on + strand, within GFF6338 at 2.984 kb on - strand, within GFF6338 at 2.984 kb on - strand, within GFF6338 at 2.984 kb on - strand, within GFF6338 at 3.006 kb on + strand at 3.162 kb on - strand at 3.162 kb on - strand at 3.162 kb on - strand at 3.359 kb on + strand at 3.360 kb on - strand at 3.385 kb on + strand at 3.396 kb on + strand at 3.397 kb on - strand at 3.399 kb on + strand at 3.399 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.635 kb on - strand at 3.635 kb on - strand at 3.635 kb on - strand at 3.635 kb on - strand at 3.635 kb on - strand at 3.635 kb on - strand at 3.639 kb on + strand at 3.639 kb on + strand at 3.640 kb on - strand at 3.686 kb on - strand at 3.737 kb on + strand at 3.737 kb on + strand at 3.738 kb on - strand at 3.774 kb on - strand at 3.780 kb on - strand at 3.797 kb on + strand at 3.797 kb on + strand at 3.798 kb on - strand at 3.896 kb on - strand, within GFF6340 at 3.896 kb on - strand, within GFF6340
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 2,005 + +0.6 2,005 + +1.3 2,006 - +0.1 2,015 + +0.7 2,015 + -2.2 2,015 + -0.2 2,046 + +0.5 2,046 + +1.0 2,060 + +1.8 2,061 - -0.7 2,061 - -0.6 2,061 - -1.4 2,061 - +0.4 2,115 + +0.6 2,116 - -1.2 2,176 + GFF6337 0.15 +0.8 2,176 + GFF6337 0.15 +1.2 2,177 - GFF6337 0.15 +1.5 2,177 - GFF6337 0.15 +1.7 2,177 - GFF6337 0.15 +0.0 2,177 - GFF6337 0.15 -0.7 2,341 + GFF6337 0.35 +0.2 2,341 + GFF6337 0.35 +0.1 2,342 - GFF6337 0.35 -2.0 2,342 - GFF6337 0.35 +0.3 2,374 + GFF6337 0.39 -1.4 2,375 - GFF6337 0.39 +1.0 2,375 - GFF6337 0.39 -1.2 2,389 + GFF6337 0.41 -0.1 2,389 + GFF6337 0.41 -0.8 2,389 + GFF6337 0.41 -0.0 2,389 + GFF6337 0.41 -0.9 2,389 + GFF6337 0.41 -0.6 2,390 - GFF6337 0.41 -0.0 2,390 - GFF6337 0.41 +0.3 2,390 - GFF6337 0.41 -0.2 2,390 - GFF6337 0.41 -0.0 2,390 - GFF6337 0.41 -0.2 2,390 - GFF6337 0.41 -1.9 2,409 - GFF6337 0.43 -0.7 2,409 - GFF6337 0.43 +1.5 2,467 - GFF6337 0.50 -0.0 2,688 + GFF6337 0.76 -0.5 2,689 - GFF6337 0.76 -0.1 2,689 - GFF6337 0.76 +0.5 2,814 + +0.5 2,814 + +0.1 2,983 + GFF6338 0.77 +0.5 2,983 + GFF6338 0.77 +1.0 2,983 + GFF6338 0.77 -0.0 2,983 + GFF6338 0.77 +0.0 2,984 - GFF6338 0.78 -0.3 2,984 - GFF6338 0.78 -1.2 2,984 - GFF6338 0.78 -0.2 3,006 + +0.0 3,162 - -0.9 3,162 - +0.1 3,162 - +1.0 3,359 + -0.0 3,360 - +1.5 3,385 + +0.0 3,396 + -0.4 3,397 - +0.2 3,399 + +1.8 3,399 + -0.1 3,568 + +0.2 3,568 + +0.1 3,568 + +1.3 3,569 - -0.5 3,569 - -0.3 3,569 - -0.4 3,569 - +1.0 3,569 - +0.0 3,569 - +0.1 3,635 - +0.0 3,635 - -0.3 3,635 - -0.1 3,635 - -0.1 3,635 - +0.2 3,635 - -0.2 3,639 + -0.4 3,639 + -0.1 3,640 - -0.2 3,686 - -0.3 3,737 + -1.7 3,737 + +1.0 3,738 - +0.3 3,774 - +1.6 3,780 - +0.3 3,797 + -0.7 3,797 + +0.5 3,798 - +1.0 3,896 - GFF6340 0.58 +0.0 3,896 - GFF6340 0.58 -0.4
Or see this region's nucleotide sequence