Experiment: TP7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dauA and prs are separated by 151 nucleotides prs and ispE are separated by 84 nucleotides ispE and lolB overlap by 1 nucleotides
HEPCGN_24890: dauA - C4-dicarboxylic acid transporter DauA, at 3,733,295 to 3,734,947
dauA
HEPCGN_24895: prs - ribose-phosphate diphosphokinase, at 3,735,099 to 3,736,112
prs
HEPCGN_24900: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 3,736,197 to 3,737,048
ispE
HEPCGN_24905: lolB - lipoprotein insertase outer membrane protein LolB, at 3,737,048 to 3,737,671
lolB
Position (kb)
3735
3736
3737 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3734.153 kb on - strand, within dauA at 3734.153 kb on - strand, within dauA at 3734.186 kb on + strand, within dauA at 3734.227 kb on + strand, within dauA at 3734.281 kb on + strand, within dauA at 3734.281 kb on + strand, within dauA at 3734.364 kb on - strand, within dauA at 3734.453 kb on + strand, within dauA at 3734.454 kb on - strand, within dauA at 3734.475 kb on + strand, within dauA at 3734.476 kb on - strand, within dauA at 3734.531 kb on - strand, within dauA at 3734.565 kb on - strand, within dauA at 3734.586 kb on + strand, within dauA at 3734.694 kb on + strand, within dauA at 3734.786 kb on - strand at 3734.787 kb on + strand at 3734.787 kb on + strand at 3734.787 kb on + strand at 3734.876 kb on + strand at 3734.876 kb on + strand at 3734.902 kb on + strand at 3735.011 kb on + strand at 3735.033 kb on + strand at 3735.033 kb on + strand at 3735.034 kb on - strand at 3735.040 kb on + strand at 3735.041 kb on - strand at 3735.054 kb on - strand at 3737.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP7 remove 3,734,153 - dauA HEPCGN_24890 0.52 +0.2 3,734,153 - dauA HEPCGN_24890 0.52 -0.7 3,734,186 + dauA HEPCGN_24890 0.54 +2.1 3,734,227 + dauA HEPCGN_24890 0.56 -1.3 3,734,281 + dauA HEPCGN_24890 0.60 +1.0 3,734,281 + dauA HEPCGN_24890 0.60 +1.7 3,734,364 - dauA HEPCGN_24890 0.65 -0.3 3,734,453 + dauA HEPCGN_24890 0.70 +0.2 3,734,454 - dauA HEPCGN_24890 0.70 -2.9 3,734,475 + dauA HEPCGN_24890 0.71 -0.7 3,734,476 - dauA HEPCGN_24890 0.71 -1.3 3,734,531 - dauA HEPCGN_24890 0.75 -1.0 3,734,565 - dauA HEPCGN_24890 0.77 -1.8 3,734,586 + dauA HEPCGN_24890 0.78 +2.0 3,734,694 + dauA HEPCGN_24890 0.85 +0.2 3,734,786 - +1.0 3,734,787 + -0.6 3,734,787 + +2.7 3,734,787 + +1.3 3,734,876 + +1.6 3,734,876 + +1.3 3,734,902 + +1.3 3,735,011 + -0.3 3,735,033 + +0.7 3,735,033 + -0.5 3,735,034 - +1.2 3,735,040 + +1.7 3,735,041 - -0.3 3,735,054 - +3.8 3,737,050 - +0.2
Or see this region's nucleotide sequence