Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntB and pntA overlap by 1 nucleotides pntA and rpoE are separated by 54 nucleotides rpoE and OKGIIK_10690 overlap by 4 nucleotides OKGIIK_10690 and OKGIIK_10695 are separated by 3 nucleotides
OKGIIK_10675: pntB - NAD(P) transhydrogenase subunit beta, at 2,289,507 to 2,290,964
pntB
OKGIIK_10680: pntA - NAD(P)(+) transhydrogenase, at 2,290,964 to 2,291,269
pntA
OKGIIK_10685: rpoE - RNA polymerase sigma factor, at 2,291,324 to 2,292,004
rpoE
OKGIIK_10690: OKGIIK_10690 - hypothetical protein, at 2,292,001 to 2,292,390
_10690
OKGIIK_10695: OKGIIK_10695 - DUF3106 domain-containing protein, at 2,292,394 to 2,292,933
_10695
Position (kb)
2291
2292
2293 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2290.595 kb on + strand, within pntB at 2290.596 kb on - strand, within pntB at 2290.690 kb on + strand, within pntB at 2290.690 kb on + strand, within pntB at 2290.690 kb on + strand, within pntB at 2290.691 kb on - strand, within pntB at 2290.691 kb on - strand, within pntB at 2290.691 kb on - strand, within pntB at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.930 kb on + strand at 2290.931 kb on - strand at 2290.931 kb on - strand at 2290.931 kb on - strand at 2290.931 kb on - strand at 2290.931 kb on - strand at 2290.931 kb on - strand at 2291.034 kb on + strand, within pntA at 2291.034 kb on + strand, within pntA at 2291.034 kb on + strand, within pntA at 2291.035 kb on - strand, within pntA at 2291.035 kb on - strand, within pntA at 2291.035 kb on - strand, within pntA at 2291.179 kb on + strand, within pntA at 2291.179 kb on + strand, within pntA at 2291.179 kb on + strand, within pntA at 2291.179 kb on + strand, within pntA at 2291.179 kb on + strand, within pntA at 2291.179 kb on + strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.180 kb on - strand, within pntA at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.244 kb on + strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.245 kb on - strand at 2291.319 kb on + strand at 2291.319 kb on + strand at 2291.320 kb on - strand at 2291.320 kb on - strand at 2291.320 kb on - strand at 2291.320 kb on - strand at 2291.320 kb on - strand at 2291.320 kb on - strand at 2291.320 kb on - strand at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.396 kb on + strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.397 kb on - strand, within rpoE at 2291.442 kb on - strand, within rpoE at 2291.443 kb on - strand, within rpoE at 2291.443 kb on - strand, within rpoE at 2291.443 kb on - strand, within rpoE at 2291.607 kb on - strand, within rpoE at 2291.607 kb on - strand, within rpoE at 2291.816 kb on + strand, within rpoE at 2291.816 kb on + strand, within rpoE at 2291.816 kb on + strand, within rpoE at 2291.816 kb on + strand, within rpoE at 2291.817 kb on - strand, within rpoE at 2291.881 kb on + strand, within rpoE at 2291.881 kb on + strand, within rpoE at 2291.881 kb on + strand, within rpoE at 2291.881 kb on + strand, within rpoE at 2291.882 kb on - strand, within rpoE at 2292.941 kb on - strand at 2292.967 kb on - strand at 2292.967 kb on - strand at 2292.967 kb on - strand at 2292.967 kb on - strand at 2292.967 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,290,595 + pntB OKGIIK_10675 0.75 -0.5 2,290,596 - pntB OKGIIK_10675 0.75 +1.0 2,290,690 + pntB OKGIIK_10675 0.81 +1.0 2,290,690 + pntB OKGIIK_10675 0.81 -0.2 2,290,690 + pntB OKGIIK_10675 0.81 -0.7 2,290,691 - pntB OKGIIK_10675 0.81 -0.5 2,290,691 - pntB OKGIIK_10675 0.81 +0.0 2,290,691 - pntB OKGIIK_10675 0.81 +0.2 2,290,930 + +1.0 2,290,930 + +0.4 2,290,930 + -0.8 2,290,930 + +1.6 2,290,930 + +0.6 2,290,930 + -0.2 2,290,930 + -0.6 2,290,930 + +1.3 2,290,931 - +0.5 2,290,931 - -1.4 2,290,931 - -0.5 2,290,931 - -1.2 2,290,931 - -1.2 2,290,931 - -0.2 2,291,034 + pntA OKGIIK_10680 0.23 -1.6 2,291,034 + pntA OKGIIK_10680 0.23 +0.3 2,291,034 + pntA OKGIIK_10680 0.23 +1.0 2,291,035 - pntA OKGIIK_10680 0.23 -1.1 2,291,035 - pntA OKGIIK_10680 0.23 +0.9 2,291,035 - pntA OKGIIK_10680 0.23 +1.3 2,291,179 + pntA OKGIIK_10680 0.70 +0.8 2,291,179 + pntA OKGIIK_10680 0.70 +0.3 2,291,179 + pntA OKGIIK_10680 0.70 -0.7 2,291,179 + pntA OKGIIK_10680 0.70 -0.5 2,291,179 + pntA OKGIIK_10680 0.70 -1.1 2,291,179 + pntA OKGIIK_10680 0.70 +0.3 2,291,180 - pntA OKGIIK_10680 0.71 +1.3 2,291,180 - pntA OKGIIK_10680 0.71 -0.2 2,291,180 - pntA OKGIIK_10680 0.71 +0.5 2,291,180 - pntA OKGIIK_10680 0.71 -0.5 2,291,180 - pntA OKGIIK_10680 0.71 +0.0 2,291,180 - pntA OKGIIK_10680 0.71 -0.7 2,291,180 - pntA OKGIIK_10680 0.71 +1.9 2,291,180 - pntA OKGIIK_10680 0.71 +0.0 2,291,180 - pntA OKGIIK_10680 0.71 +1.2 2,291,180 - pntA OKGIIK_10680 0.71 +0.9 2,291,244 + +0.3 2,291,244 + +0.7 2,291,244 + -0.5 2,291,244 + +0.3 2,291,244 + +0.3 2,291,244 + -0.2 2,291,244 + -0.4 2,291,244 + +0.0 2,291,244 + -2.2 2,291,244 + -0.1 2,291,245 - +0.3 2,291,245 - -2.2 2,291,245 - +1.0 2,291,245 - +0.3 2,291,245 - +0.3 2,291,245 - -1.4 2,291,245 - -0.4 2,291,245 - +0.5 2,291,245 - -0.1 2,291,245 - -0.8 2,291,245 - +0.1 2,291,245 - +1.3 2,291,245 - -0.9 2,291,245 - +0.3 2,291,245 - -1.4 2,291,245 - -0.5 2,291,245 - +0.9 2,291,245 - -0.1 2,291,245 - -1.7 2,291,245 - +0.8 2,291,245 - -0.2 2,291,245 - -0.2 2,291,319 + +1.9 2,291,319 + +0.0 2,291,320 - -0.4 2,291,320 - -1.0 2,291,320 - -1.1 2,291,320 - +0.9 2,291,320 - +0.6 2,291,320 - +0.0 2,291,320 - +0.9 2,291,396 + rpoE OKGIIK_10685 0.11 +0.0 2,291,396 + rpoE OKGIIK_10685 0.11 -0.5 2,291,396 + rpoE OKGIIK_10685 0.11 -1.0 2,291,396 + rpoE OKGIIK_10685 0.11 -0.8 2,291,396 + rpoE OKGIIK_10685 0.11 +1.5 2,291,396 + rpoE OKGIIK_10685 0.11 -0.7 2,291,396 + rpoE OKGIIK_10685 0.11 -2.4 2,291,396 + rpoE OKGIIK_10685 0.11 -1.0 2,291,396 + rpoE OKGIIK_10685 0.11 -1.7 2,291,397 - rpoE OKGIIK_10685 0.11 -1.7 2,291,397 - rpoE OKGIIK_10685 0.11 +0.3 2,291,397 - rpoE OKGIIK_10685 0.11 +0.8 2,291,397 - rpoE OKGIIK_10685 0.11 +0.0 2,291,397 - rpoE OKGIIK_10685 0.11 -0.2 2,291,397 - rpoE OKGIIK_10685 0.11 -1.6 2,291,397 - rpoE OKGIIK_10685 0.11 -0.8 2,291,397 - rpoE OKGIIK_10685 0.11 +0.8 2,291,397 - rpoE OKGIIK_10685 0.11 -1.0 2,291,397 - rpoE OKGIIK_10685 0.11 -0.8 2,291,397 - rpoE OKGIIK_10685 0.11 +0.5 2,291,397 - rpoE OKGIIK_10685 0.11 +1.1 2,291,397 - rpoE OKGIIK_10685 0.11 -1.4 2,291,397 - rpoE OKGIIK_10685 0.11 -0.8 2,291,442 - rpoE OKGIIK_10685 0.17 -0.7 2,291,443 - rpoE OKGIIK_10685 0.17 -1.5 2,291,443 - rpoE OKGIIK_10685 0.17 -2.3 2,291,443 - rpoE OKGIIK_10685 0.17 +0.8 2,291,607 - rpoE OKGIIK_10685 0.42 -0.7 2,291,607 - rpoE OKGIIK_10685 0.42 -1.1 2,291,816 + rpoE OKGIIK_10685 0.72 -0.1 2,291,816 + rpoE OKGIIK_10685 0.72 +1.5 2,291,816 + rpoE OKGIIK_10685 0.72 -1.4 2,291,816 + rpoE OKGIIK_10685 0.72 -0.1 2,291,817 - rpoE OKGIIK_10685 0.72 -0.8 2,291,881 + rpoE OKGIIK_10685 0.82 +0.3 2,291,881 + rpoE OKGIIK_10685 0.82 -0.5 2,291,881 + rpoE OKGIIK_10685 0.82 +0.1 2,291,881 + rpoE OKGIIK_10685 0.82 +1.9 2,291,882 - rpoE OKGIIK_10685 0.82 +1.1 2,292,941 - -1.1 2,292,967 - +1.4 2,292,967 - +0.0 2,292,967 - -0.2 2,292,967 - +0.6 2,292,967 - +2.6
Or see this region's nucleotide sequence