Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fumE and coaA overlap by 4 nucleotides coaA and cysA overlap by 29 nucleotides cysA and ecfA2 overlap by 7 nucleotides ecfA2 and BNILDI_09820 overlap by 13 nucleotides
BNILDI_09800: fumE - fumarase E, at 1,973,293 to 1,973,802
fumE
BNILDI_09805: coaA - nucleoside/nucleotide kinase family protein, at 1,973,799 to 1,974,512
coaA
BNILDI_09810: cysA - ABC transporter ATP-binding protein, at 1,974,484 to 1,975,161
cysA
BNILDI_09815: ecfA2 - cobalt ABC transporter ATP-binding protein, at 1,975,155 to 1,975,832
ecfA2
BNILDI_09820: BNILDI_09820 - cobalt ABC transporter permease, at 1,975,820 to 1,976,527
_09820
Position (kb)
1974
1975
1976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1973.536 kb on - strand, within fumE at 1973.545 kb on + strand, within fumE at 1973.784 kb on - strand at 1973.873 kb on + strand, within coaA at 1973.911 kb on + strand, within coaA at 1973.927 kb on + strand, within coaA at 1973.927 kb on + strand, within coaA at 1973.969 kb on + strand, within coaA at 1973.970 kb on - strand, within coaA at 1973.975 kb on - strand, within coaA at 1973.975 kb on - strand, within coaA at 1973.979 kb on - strand, within coaA at 1973.992 kb on - strand, within coaA at 1973.992 kb on - strand, within coaA at 1974.058 kb on - strand, within coaA at 1974.063 kb on - strand, within coaA at 1974.167 kb on + strand, within coaA at 1974.168 kb on - strand, within coaA at 1974.168 kb on - strand, within coaA at 1974.405 kb on + strand, within coaA at 1974.568 kb on - strand, within cysA at 1974.814 kb on - strand, within cysA at 1975.278 kb on + strand, within ecfA2 at 1975.278 kb on + strand, within ecfA2 at 1975.541 kb on + strand, within ecfA2 at 1975.653 kb on + strand, within ecfA2 at 1975.759 kb on + strand, within ecfA2 at 1975.760 kb on - strand, within ecfA2 at 1975.821 kb on + strand at 1975.826 kb on + strand at 1975.826 kb on + strand at 1975.892 kb on + strand, within BNILDI_09820 at 1975.987 kb on + strand, within BNILDI_09820 at 1976.111 kb on + strand, within BNILDI_09820 at 1976.112 kb on - strand, within BNILDI_09820 at 1976.148 kb on + strand, within BNILDI_09820 at 1976.148 kb on + strand, within BNILDI_09820 at 1976.149 kb on - strand, within BNILDI_09820
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,973,536 - fumE BNILDI_09800 0.48 -1.5 1,973,545 + fumE BNILDI_09800 0.49 -1.3 1,973,784 - +0.4 1,973,873 + coaA BNILDI_09805 0.10 +1.2 1,973,911 + coaA BNILDI_09805 0.16 -1.8 1,973,927 + coaA BNILDI_09805 0.18 -0.4 1,973,927 + coaA BNILDI_09805 0.18 -0.4 1,973,969 + coaA BNILDI_09805 0.24 -0.0 1,973,970 - coaA BNILDI_09805 0.24 +0.5 1,973,975 - coaA BNILDI_09805 0.25 -0.8 1,973,975 - coaA BNILDI_09805 0.25 -0.5 1,973,979 - coaA BNILDI_09805 0.25 -0.5 1,973,992 - coaA BNILDI_09805 0.27 +0.4 1,973,992 - coaA BNILDI_09805 0.27 -0.8 1,974,058 - coaA BNILDI_09805 0.36 -0.5 1,974,063 - coaA BNILDI_09805 0.37 -0.1 1,974,167 + coaA BNILDI_09805 0.52 +0.0 1,974,168 - coaA BNILDI_09805 0.52 -0.7 1,974,168 - coaA BNILDI_09805 0.52 +0.5 1,974,405 + coaA BNILDI_09805 0.85 +0.3 1,974,568 - cysA BNILDI_09810 0.12 -2.9 1,974,814 - cysA BNILDI_09810 0.49 +0.7 1,975,278 + ecfA2 BNILDI_09815 0.18 +0.1 1,975,278 + ecfA2 BNILDI_09815 0.18 +1.0 1,975,541 + ecfA2 BNILDI_09815 0.57 -0.2 1,975,653 + ecfA2 BNILDI_09815 0.73 -2.1 1,975,759 + ecfA2 BNILDI_09815 0.89 -0.6 1,975,760 - ecfA2 BNILDI_09815 0.89 -0.2 1,975,821 + +0.1 1,975,826 + -0.0 1,975,826 + -0.5 1,975,892 + BNILDI_09820 0.10 +1.9 1,975,987 + BNILDI_09820 0.24 -1.1 1,976,111 + BNILDI_09820 0.41 -1.0 1,976,112 - BNILDI_09820 0.41 +0.3 1,976,148 + BNILDI_09820 0.46 +0.5 1,976,148 + BNILDI_09820 0.46 +0.4 1,976,149 - BNILDI_09820 0.46 +2.2
Or see this region's nucleotide sequence