Strain Fitness in Escherichia coli ECRC62 around BNILDI_09265

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttsaA and rcsF overlap by 4 nucleotidesrcsF and metQ are separated by 117 nucleotidesmetQ and metI are separated by 39 nucleotidesmetI and metN overlap by 8 nucleotides BNILDI_09255: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,857,753 to 1,858,460 tsaA BNILDI_09260: rcsF - Rcs stress response system protein RcsF, at 1,858,457 to 1,858,861 rcsF BNILDI_09265: metQ - methionine ABC transporter substrate-binding lipoprotein MetQ, at 1,858,979 to 1,859,794 metQ BNILDI_09270: metI - D-methionine transport system permease protein MetI, at 1,859,834 to 1,860,487 metI BNILDI_09275: metN - methionine ABC transporter ATP-binding protein MetN, at 1,860,480 to 1,861,511 metN Position (kb) 1858 1859 1860Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1858.048 kb on + strand, within tsaAat 1858.157 kb on + strand, within tsaAat 1858.290 kb on + strand, within tsaAat 1858.291 kb on - strand, within tsaAat 1858.336 kb on + strand, within tsaAat 1858.337 kb on - strand, within tsaAat 1858.414 kb on + strandat 1858.415 kb on - strandat 1858.434 kb on + strandat 1858.435 kb on - strandat 1858.435 kb on - strandat 1858.475 kb on - strandat 1858.493 kb on + strandat 1858.493 kb on + strandat 1858.494 kb on - strandat 1858.506 kb on + strand, within rcsFat 1858.540 kb on + strand, within rcsFat 1858.547 kb on + strand, within rcsFat 1858.547 kb on + strand, within rcsFat 1858.547 kb on + strand, within rcsFat 1858.548 kb on - strand, within rcsFat 1858.631 kb on - strand, within rcsFat 1858.705 kb on + strand, within rcsFat 1858.836 kb on - strandat 1858.861 kb on + strandat 1858.861 kb on + strandat 1858.862 kb on - strandat 1858.862 kb on - strandat 1858.864 kb on + strandat 1858.877 kb on + strandat 1858.878 kb on - strandat 1858.912 kb on + strandat 1858.950 kb on + strandat 1858.951 kb on - strandat 1859.086 kb on + strand, within metQat 1859.111 kb on + strand, within metQat 1859.181 kb on + strand, within metQat 1859.181 kb on + strand, within metQat 1859.182 kb on - strand, within metQat 1859.194 kb on + strand, within metQat 1859.194 kb on + strand, within metQat 1859.194 kb on + strand, within metQat 1859.195 kb on - strand, within metQat 1859.478 kb on - strand, within metQat 1859.524 kb on - strand, within metQat 1859.530 kb on + strand, within metQat 1859.626 kb on - strand, within metQat 1859.626 kb on - strand, within metQat 1859.626 kb on - strand, within metQat 1859.696 kb on - strand, within metQat 1859.696 kb on - strand, within metQat 1859.794 kb on + strandat 1859.814 kb on + strandat 1859.819 kb on - strandat 1859.882 kb on + strandat 1859.882 kb on + strandat 1859.883 kb on - strandat 1859.883 kb on - strandat 1859.917 kb on + strand, within metIat 1859.946 kb on + strand, within metIat 1859.946 kb on + strand, within metIat 1860.245 kb on - strand, within metIat 1860.281 kb on - strand, within metIat 1860.350 kb on - strand, within metIat 1860.372 kb on + strand, within metIat 1860.373 kb on - strand, within metIat 1860.373 kb on - strand, within metIat 1860.373 kb on - strand, within metIat 1860.487 kb on + strandat 1860.487 kb on + strandat 1860.488 kb on - strandat 1860.501 kb on + strandat 1860.501 kb on + strandat 1860.501 kb on + strandat 1860.508 kb on - strandat 1860.546 kb on - strandat 1860.621 kb on - strand, within metNat 1860.623 kb on + strand, within metNat 1860.623 kb on + strand, within metNat 1860.623 kb on + strand, within metNat 1860.623 kb on + strand, within metNat 1860.623 kb on + strand, within metNat 1860.624 kb on - strand, within metNat 1860.624 kb on - strand, within metNat 1860.725 kb on + strand, within metNat 1860.792 kb on - strand, within metN

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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1,858,048 + tsaA BNILDI_09255 0.42 +0.2
1,858,157 + tsaA BNILDI_09255 0.57 +0.9
1,858,290 + tsaA BNILDI_09255 0.76 +0.5
1,858,291 - tsaA BNILDI_09255 0.76 -1.0
1,858,336 + tsaA BNILDI_09255 0.82 -0.9
1,858,337 - tsaA BNILDI_09255 0.82 -0.9
1,858,414 + +0.6
1,858,415 - -0.0
1,858,434 + -1.7
1,858,435 - -0.8
1,858,435 - +2.9
1,858,475 - +1.9
1,858,493 + +1.3
1,858,493 + -0.8
1,858,494 - +1.5
1,858,506 + rcsF BNILDI_09260 0.12 -1.1
1,858,540 + rcsF BNILDI_09260 0.20 +0.3
1,858,547 + rcsF BNILDI_09260 0.22 -1.6
1,858,547 + rcsF BNILDI_09260 0.22 -1.0
1,858,547 + rcsF BNILDI_09260 0.22 -0.9
1,858,548 - rcsF BNILDI_09260 0.22 +0.1
1,858,631 - rcsF BNILDI_09260 0.43 -0.5
1,858,705 + rcsF BNILDI_09260 0.61 -0.3
1,858,836 - +1.1
1,858,861 + -0.5
1,858,861 + +0.2
1,858,862 - -0.1
1,858,862 - +1.9
1,858,864 + -0.3
1,858,877 + +0.5
1,858,878 - -0.2
1,858,912 + +0.7
1,858,950 + +1.4
1,858,951 - -1.1
1,859,086 + metQ BNILDI_09265 0.13 -1.0
1,859,111 + metQ BNILDI_09265 0.16 -1.0
1,859,181 + metQ BNILDI_09265 0.25 -1.7
1,859,181 + metQ BNILDI_09265 0.25 -0.0
1,859,182 - metQ BNILDI_09265 0.25 -2.5
1,859,194 + metQ BNILDI_09265 0.26 -0.3
1,859,194 + metQ BNILDI_09265 0.26 -0.5
1,859,194 + metQ BNILDI_09265 0.26 -0.3
1,859,195 - metQ BNILDI_09265 0.26 -1.1
1,859,478 - metQ BNILDI_09265 0.61 -0.6
1,859,524 - metQ BNILDI_09265 0.67 -0.5
1,859,530 + metQ BNILDI_09265 0.68 -1.6
1,859,626 - metQ BNILDI_09265 0.79 -0.4
1,859,626 - metQ BNILDI_09265 0.79 -0.7
1,859,626 - metQ BNILDI_09265 0.79 -2.1
1,859,696 - metQ BNILDI_09265 0.88 +0.1
1,859,696 - metQ BNILDI_09265 0.88 -0.3
1,859,794 + +0.9
1,859,814 + -0.2
1,859,819 - -1.8
1,859,882 + +0.6
1,859,882 + -0.2
1,859,883 - -1.1
1,859,883 - -0.3
1,859,917 + metI BNILDI_09270 0.13 +0.1
1,859,946 + metI BNILDI_09270 0.17 +1.3
1,859,946 + metI BNILDI_09270 0.17 +1.0
1,860,245 - metI BNILDI_09270 0.63 +0.3
1,860,281 - metI BNILDI_09270 0.68 +1.1
1,860,350 - metI BNILDI_09270 0.79 -0.1
1,860,372 + metI BNILDI_09270 0.82 -0.5
1,860,373 - metI BNILDI_09270 0.82 -0.6
1,860,373 - metI BNILDI_09270 0.82 -0.5
1,860,373 - metI BNILDI_09270 0.82 -0.3
1,860,487 + -0.3
1,860,487 + -0.0
1,860,488 - -1.2
1,860,501 + +0.5
1,860,501 + -0.9
1,860,501 + -0.2
1,860,508 - +0.6
1,860,546 - -0.4
1,860,621 - metN BNILDI_09275 0.14 -0.2
1,860,623 + metN BNILDI_09275 0.14 -1.2
1,860,623 + metN BNILDI_09275 0.14 +0.1
1,860,623 + metN BNILDI_09275 0.14 -1.7
1,860,623 + metN BNILDI_09275 0.14 -0.0
1,860,623 + metN BNILDI_09275 0.14 +1.9
1,860,624 - metN BNILDI_09275 0.14 +0.3
1,860,624 - metN BNILDI_09275 0.14 +1.9
1,860,725 + metN BNILDI_09275 0.24 +1.0
1,860,792 - metN BNILDI_09275 0.30 +0.0

Or see this region's nucleotide sequence