Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsaA and rcsF overlap by 4 nucleotides rcsF and metQ are separated by 117 nucleotides metQ and metI are separated by 39 nucleotides metI and metN overlap by 8 nucleotides
BNILDI_09255: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,857,753 to 1,858,460
tsaA
BNILDI_09260: rcsF - Rcs stress response system protein RcsF, at 1,858,457 to 1,858,861
rcsF
BNILDI_09265: metQ - methionine ABC transporter substrate-binding lipoprotein MetQ, at 1,858,979 to 1,859,794
metQ
BNILDI_09270: metI - D-methionine transport system permease protein MetI, at 1,859,834 to 1,860,487
metI
BNILDI_09275: metN - methionine ABC transporter ATP-binding protein MetN, at 1,860,480 to 1,861,511
metN
Position (kb)
1858
1859
1860 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1858.048 kb on + strand, within tsaA at 1858.157 kb on + strand, within tsaA at 1858.290 kb on + strand, within tsaA at 1858.291 kb on - strand, within tsaA at 1858.336 kb on + strand, within tsaA at 1858.337 kb on - strand, within tsaA at 1858.414 kb on + strand at 1858.415 kb on - strand at 1858.434 kb on + strand at 1858.435 kb on - strand at 1858.435 kb on - strand at 1858.475 kb on - strand at 1858.493 kb on + strand at 1858.493 kb on + strand at 1858.494 kb on - strand at 1858.506 kb on + strand, within rcsF at 1858.540 kb on + strand, within rcsF at 1858.547 kb on + strand, within rcsF at 1858.547 kb on + strand, within rcsF at 1858.547 kb on + strand, within rcsF at 1858.548 kb on - strand, within rcsF at 1858.631 kb on - strand, within rcsF at 1858.705 kb on + strand, within rcsF at 1858.836 kb on - strand at 1858.861 kb on + strand at 1858.861 kb on + strand at 1858.862 kb on - strand at 1858.862 kb on - strand at 1858.864 kb on + strand at 1858.877 kb on + strand at 1858.878 kb on - strand at 1858.912 kb on + strand at 1858.950 kb on + strand at 1858.951 kb on - strand at 1859.086 kb on + strand, within metQ at 1859.111 kb on + strand, within metQ at 1859.181 kb on + strand, within metQ at 1859.181 kb on + strand, within metQ at 1859.182 kb on - strand, within metQ at 1859.194 kb on + strand, within metQ at 1859.194 kb on + strand, within metQ at 1859.194 kb on + strand, within metQ at 1859.195 kb on - strand, within metQ at 1859.478 kb on - strand, within metQ at 1859.524 kb on - strand, within metQ at 1859.530 kb on + strand, within metQ at 1859.626 kb on - strand, within metQ at 1859.626 kb on - strand, within metQ at 1859.626 kb on - strand, within metQ at 1859.696 kb on - strand, within metQ at 1859.696 kb on - strand, within metQ at 1859.794 kb on + strand at 1859.814 kb on + strand at 1859.819 kb on - strand at 1859.882 kb on + strand at 1859.882 kb on + strand at 1859.883 kb on - strand at 1859.883 kb on - strand at 1859.917 kb on + strand, within metI at 1859.946 kb on + strand, within metI at 1859.946 kb on + strand, within metI at 1860.245 kb on - strand, within metI at 1860.281 kb on - strand, within metI at 1860.350 kb on - strand, within metI at 1860.372 kb on + strand, within metI at 1860.373 kb on - strand, within metI at 1860.373 kb on - strand, within metI at 1860.373 kb on - strand, within metI at 1860.487 kb on + strand at 1860.487 kb on + strand at 1860.488 kb on - strand at 1860.501 kb on + strand at 1860.501 kb on + strand at 1860.501 kb on + strand at 1860.508 kb on - strand at 1860.546 kb on - strand at 1860.621 kb on - strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.623 kb on + strand, within metN at 1860.624 kb on - strand, within metN at 1860.624 kb on - strand, within metN at 1860.725 kb on + strand, within metN at 1860.792 kb on - strand, within metN
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,858,048 + tsaA BNILDI_09255 0.42 +0.2 1,858,157 + tsaA BNILDI_09255 0.57 +0.9 1,858,290 + tsaA BNILDI_09255 0.76 +0.5 1,858,291 - tsaA BNILDI_09255 0.76 -1.0 1,858,336 + tsaA BNILDI_09255 0.82 -0.9 1,858,337 - tsaA BNILDI_09255 0.82 -0.9 1,858,414 + +0.6 1,858,415 - -0.0 1,858,434 + -1.7 1,858,435 - -0.8 1,858,435 - +2.9 1,858,475 - +1.9 1,858,493 + +1.3 1,858,493 + -0.8 1,858,494 - +1.5 1,858,506 + rcsF BNILDI_09260 0.12 -1.1 1,858,540 + rcsF BNILDI_09260 0.20 +0.3 1,858,547 + rcsF BNILDI_09260 0.22 -1.6 1,858,547 + rcsF BNILDI_09260 0.22 -1.0 1,858,547 + rcsF BNILDI_09260 0.22 -0.9 1,858,548 - rcsF BNILDI_09260 0.22 +0.1 1,858,631 - rcsF BNILDI_09260 0.43 -0.5 1,858,705 + rcsF BNILDI_09260 0.61 -0.3 1,858,836 - +1.1 1,858,861 + -0.5 1,858,861 + +0.2 1,858,862 - -0.1 1,858,862 - +1.9 1,858,864 + -0.3 1,858,877 + +0.5 1,858,878 - -0.2 1,858,912 + +0.7 1,858,950 + +1.4 1,858,951 - -1.1 1,859,086 + metQ BNILDI_09265 0.13 -1.0 1,859,111 + metQ BNILDI_09265 0.16 -1.0 1,859,181 + metQ BNILDI_09265 0.25 -1.7 1,859,181 + metQ BNILDI_09265 0.25 -0.0 1,859,182 - metQ BNILDI_09265 0.25 -2.5 1,859,194 + metQ BNILDI_09265 0.26 -0.3 1,859,194 + metQ BNILDI_09265 0.26 -0.5 1,859,194 + metQ BNILDI_09265 0.26 -0.3 1,859,195 - metQ BNILDI_09265 0.26 -1.1 1,859,478 - metQ BNILDI_09265 0.61 -0.6 1,859,524 - metQ BNILDI_09265 0.67 -0.5 1,859,530 + metQ BNILDI_09265 0.68 -1.6 1,859,626 - metQ BNILDI_09265 0.79 -0.4 1,859,626 - metQ BNILDI_09265 0.79 -0.7 1,859,626 - metQ BNILDI_09265 0.79 -2.1 1,859,696 - metQ BNILDI_09265 0.88 +0.1 1,859,696 - metQ BNILDI_09265 0.88 -0.3 1,859,794 + +0.9 1,859,814 + -0.2 1,859,819 - -1.8 1,859,882 + +0.6 1,859,882 + -0.2 1,859,883 - -1.1 1,859,883 - -0.3 1,859,917 + metI BNILDI_09270 0.13 +0.1 1,859,946 + metI BNILDI_09270 0.17 +1.3 1,859,946 + metI BNILDI_09270 0.17 +1.0 1,860,245 - metI BNILDI_09270 0.63 +0.3 1,860,281 - metI BNILDI_09270 0.68 +1.1 1,860,350 - metI BNILDI_09270 0.79 -0.1 1,860,372 + metI BNILDI_09270 0.82 -0.5 1,860,373 - metI BNILDI_09270 0.82 -0.6 1,860,373 - metI BNILDI_09270 0.82 -0.5 1,860,373 - metI BNILDI_09270 0.82 -0.3 1,860,487 + -0.3 1,860,487 + -0.0 1,860,488 - -1.2 1,860,501 + +0.5 1,860,501 + -0.9 1,860,501 + -0.2 1,860,508 - +0.6 1,860,546 - -0.4 1,860,621 - metN BNILDI_09275 0.14 -0.2 1,860,623 + metN BNILDI_09275 0.14 -1.2 1,860,623 + metN BNILDI_09275 0.14 +0.1 1,860,623 + metN BNILDI_09275 0.14 -1.7 1,860,623 + metN BNILDI_09275 0.14 -0.0 1,860,623 + metN BNILDI_09275 0.14 +1.9 1,860,624 - metN BNILDI_09275 0.14 +0.3 1,860,624 - metN BNILDI_09275 0.14 +1.9 1,860,725 + metN BNILDI_09275 0.24 +1.0 1,860,792 - metN BNILDI_09275 0.30 +0.0
Or see this region's nucleotide sequence