Strain Fitness in Escherichia coli ECRC62 around BNILDI_08265

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_08255 and fimD are separated by 12 nucleotidesfimD and BNILDI_08265 are separated by 288 nucleotidesBNILDI_08265 and espX1 are separated by 432 nucleotides BNILDI_08255: BNILDI_08255 - fimbrial family protein, at 1,647,043 to 1,648,002 _08255 BNILDI_08260: fimD - fimbrial assembly protein, at 1,648,015 to 1,648,275 fimD BNILDI_08265: BNILDI_08265 - Fimbrial protein, at 1,648,564 to 1,649,160 _08265 BNILDI_08270: espX1 - type III secretion system effector EspX1, at 1,649,593 to 1,650,852 espX1 Position (kb) 1648 1649 1650Strain fitness (log2 ratio) -1 0 1at 1647.902 kb on - strand, within BNILDI_08255at 1648.171 kb on - strand, within fimDat 1648.239 kb on + strand, within fimDat 1648.252 kb on - strandat 1648.306 kb on - strandat 1648.512 kb on + strandat 1648.549 kb on - strandat 1648.676 kb on - strand, within BNILDI_08265at 1648.893 kb on + strand, within BNILDI_08265at 1648.899 kb on + strand, within BNILDI_08265at 1649.073 kb on - strand, within BNILDI_08265at 1649.088 kb on - strand, within BNILDI_08265at 1649.194 kb on - strandat 1649.194 kb on - strandat 1649.217 kb on + strandat 1649.242 kb on + strandat 1649.322 kb on + strandat 1649.324 kb on + strandat 1649.425 kb on + strandat 1649.609 kb on - strandat 1650.044 kb on + strand, within espX1

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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1,647,902 - BNILDI_08255 0.89 +0.2
1,648,171 - fimD BNILDI_08260 0.60 +0.9
1,648,239 + fimD BNILDI_08260 0.86 -1.3
1,648,252 - -0.1
1,648,306 - +1.0
1,648,512 + +0.3
1,648,549 - -0.8
1,648,676 - BNILDI_08265 0.19 -0.4
1,648,893 + BNILDI_08265 0.55 -1.0
1,648,899 + BNILDI_08265 0.56 -1.0
1,649,073 - BNILDI_08265 0.85 -0.5
1,649,088 - BNILDI_08265 0.88 -0.6
1,649,194 - +0.4
1,649,194 - +1.3
1,649,217 + +1.3
1,649,242 + +0.1
1,649,322 + -0.7
1,649,324 + -0.1
1,649,425 + +0.9
1,649,609 - -1.4
1,650,044 + espX1 BNILDI_08270 0.36 -0.7

Or see this region's nucleotide sequence