Strain Fitness in Escherichia coli ECRC62 around BNILDI_00550

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyghD and BNILDI_00545 are separated by 38 nucleotidesBNILDI_00545 and gspS2 are separated by 158 nucleotidesgspS2 and pppA are separated by 65 nucleotides BNILDI_00540: yghD - GspM family type II secretion system protein YghD, at 100,771 to 101,307 yghD BNILDI_00545: BNILDI_00545 - Type II secretion protein GspC, at 101,346 to 101,711 _00545 BNILDI_00550: gspS2 - Pilotin AspS 2, at 101,870 to 102,280 gspS2 BNILDI_00555: pppA - prepilin peptidase PppA, at 102,346 to 103,155 pppA Position (kb) 101 102 103Strain fitness (log2 ratio) -2 -1 0 1at 100.930 kb on - strand, within yghDat 101.043 kb on + strand, within yghDat 101.150 kb on + strand, within yghDat 101.150 kb on + strand, within yghDat 101.151 kb on - strand, within yghDat 101.151 kb on - strand, within yghDat 101.151 kb on - strand, within yghDat 101.151 kb on - strand, within yghDat 101.188 kb on + strand, within yghDat 101.311 kb on - strandat 101.360 kb on + strandat 101.401 kb on + strand, within BNILDI_00545at 101.401 kb on + strand, within BNILDI_00545at 101.402 kb on - strand, within BNILDI_00545at 101.478 kb on - strand, within BNILDI_00545at 101.700 kb on - strandat 101.856 kb on - strandat 101.935 kb on + strand, within gspS2at 102.070 kb on + strand, within gspS2at 102.249 kb on - strandat 102.365 kb on + strandat 102.445 kb on + strand, within pppAat 102.446 kb on - strand, within pppAat 102.446 kb on - strand, within pppAat 102.507 kb on + strand, within pppAat 102.508 kb on - strand, within pppAat 102.508 kb on - strand, within pppAat 102.508 kb on - strand, within pppAat 102.511 kb on + strand, within pppAat 102.512 kb on - strand, within pppAat 102.512 kb on - strand, within pppAat 102.512 kb on - strand, within pppAat 102.526 kb on + strand, within pppAat 102.527 kb on - strand, within pppAat 102.529 kb on + strand, within pppAat 102.530 kb on - strand, within pppAat 102.530 kb on - strand, within pppAat 102.764 kb on - strand, within pppAat 102.835 kb on + strand, within pppAat 102.922 kb on - strand, within pppAat 102.985 kb on + strand, within pppAat 103.176 kb on - strandat 103.257 kb on + strandat 103.258 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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100,930 - yghD BNILDI_00540 0.30 -1.3
101,043 + yghD BNILDI_00540 0.51 -1.2
101,150 + yghD BNILDI_00540 0.71 -0.5
101,150 + yghD BNILDI_00540 0.71 +0.1
101,151 - yghD BNILDI_00540 0.71 -0.2
101,151 - yghD BNILDI_00540 0.71 +0.2
101,151 - yghD BNILDI_00540 0.71 +0.1
101,151 - yghD BNILDI_00540 0.71 +0.2
101,188 + yghD BNILDI_00540 0.78 -0.6
101,311 - +0.5
101,360 + -0.4
101,401 + BNILDI_00545 0.15 +1.0
101,401 + BNILDI_00545 0.15 -0.1
101,402 - BNILDI_00545 0.15 +0.1
101,478 - BNILDI_00545 0.36 +0.3
101,700 - -0.1
101,856 - -0.9
101,935 + gspS2 BNILDI_00550 0.16 -0.8
102,070 + gspS2 BNILDI_00550 0.49 +0.9
102,249 - -0.1
102,365 + -1.7
102,445 + pppA BNILDI_00555 0.12 -0.1
102,446 - pppA BNILDI_00555 0.12 +0.2
102,446 - pppA BNILDI_00555 0.12 -0.2
102,507 + pppA BNILDI_00555 0.20 +0.0
102,508 - pppA BNILDI_00555 0.20 +0.2
102,508 - pppA BNILDI_00555 0.20 -1.7
102,508 - pppA BNILDI_00555 0.20 +0.0
102,511 + pppA BNILDI_00555 0.20 -0.3
102,512 - pppA BNILDI_00555 0.20 -0.4
102,512 - pppA BNILDI_00555 0.20 -1.3
102,512 - pppA BNILDI_00555 0.20 -1.6
102,526 + pppA BNILDI_00555 0.22 +0.3
102,527 - pppA BNILDI_00555 0.22 -0.6
102,529 + pppA BNILDI_00555 0.23 -0.7
102,530 - pppA BNILDI_00555 0.23 +0.3
102,530 - pppA BNILDI_00555 0.23 -0.7
102,764 - pppA BNILDI_00555 0.52 +0.4
102,835 + pppA BNILDI_00555 0.60 -2.3
102,922 - pppA BNILDI_00555 0.71 -0.3
102,985 + pppA BNILDI_00555 0.79 -0.2
103,176 - +0.7
103,257 + +0.7
103,258 - -0.5

Or see this region's nucleotide sequence