Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_03089

Experiment: N4 MOI10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_03087 and OHPLBJKB_03088 are separated by 14 nucleotidesOHPLBJKB_03088 and OHPLBJKB_03089 overlap by 4 nucleotidesOHPLBJKB_03089 and OHPLBJKB_03090 are separated by 10 nucleotides OHPLBJKB_03087: OHPLBJKB_03087 - [Citrate [pro-3S]-lyase] ligase, at 3,214,702 to 3,215,760 _03087 OHPLBJKB_03088: OHPLBJKB_03088 - Citrate lyase acyl carrier protein, at 3,215,775 to 3,216,071 _03088 OHPLBJKB_03089: OHPLBJKB_03089 - Citrate lyase subunit beta, at 3,216,068 to 3,216,976 _03089 OHPLBJKB_03090: OHPLBJKB_03090 - Citrate lyase alpha chain, at 3,216,987 to 3,218,519 _03090 Position (kb) 3216 3217Strain fitness (log2 ratio) -2 -1 0 1 2at 3215.291 kb on + strand, within OHPLBJKB_03087at 3215.371 kb on + strand, within OHPLBJKB_03087at 3215.371 kb on + strand, within OHPLBJKB_03087at 3215.371 kb on + strand, within OHPLBJKB_03087at 3215.372 kb on - strand, within OHPLBJKB_03087at 3215.407 kb on + strand, within OHPLBJKB_03087at 3215.407 kb on + strand, within OHPLBJKB_03087at 3215.407 kb on + strand, within OHPLBJKB_03087at 3215.408 kb on - strand, within OHPLBJKB_03087at 3215.462 kb on + strand, within OHPLBJKB_03087at 3215.462 kb on + strand, within OHPLBJKB_03087at 3215.463 kb on - strand, within OHPLBJKB_03087at 3215.513 kb on - strand, within OHPLBJKB_03087at 3215.524 kb on - strand, within OHPLBJKB_03087at 3215.783 kb on - strandat 3215.940 kb on + strand, within OHPLBJKB_03088at 3215.999 kb on - strand, within OHPLBJKB_03088at 3215.999 kb on - strand, within OHPLBJKB_03088at 3216.094 kb on + strandat 3216.094 kb on + strandat 3216.095 kb on - strandat 3216.566 kb on + strand, within OHPLBJKB_03089at 3216.566 kb on + strand, within OHPLBJKB_03089at 3216.566 kb on + strand, within OHPLBJKB_03089at 3216.567 kb on - strand, within OHPLBJKB_03089at 3216.567 kb on - strand, within OHPLBJKB_03089at 3216.567 kb on - strand, within OHPLBJKB_03089at 3216.584 kb on - strand, within OHPLBJKB_03089at 3216.841 kb on - strand, within OHPLBJKB_03089at 3217.223 kb on + strand, within OHPLBJKB_03090at 3217.309 kb on + strand, within OHPLBJKB_03090at 3217.344 kb on + strand, within OHPLBJKB_03090at 3217.344 kb on + strand, within OHPLBJKB_03090at 3217.344 kb on + strand, within OHPLBJKB_03090at 3217.344 kb on + strand, within OHPLBJKB_03090at 3217.345 kb on - strand, within OHPLBJKB_03090at 3217.345 kb on - strand, within OHPLBJKB_03090at 3217.345 kb on - strand, within OHPLBJKB_03090at 3217.345 kb on - strand, within OHPLBJKB_03090at 3217.444 kb on + strand, within OHPLBJKB_03090at 3217.521 kb on + strand, within OHPLBJKB_03090at 3217.718 kb on + strand, within OHPLBJKB_03090

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Per-strain Table

Position Strand Gene LocusTag Fraction N4 MOI10
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3,215,291 + OHPLBJKB_03087 0.56 -0.0
3,215,371 + OHPLBJKB_03087 0.63 +0.5
3,215,371 + OHPLBJKB_03087 0.63 -0.6
3,215,371 + OHPLBJKB_03087 0.63 -0.0
3,215,372 - OHPLBJKB_03087 0.63 -0.5
3,215,407 + OHPLBJKB_03087 0.67 -0.6
3,215,407 + OHPLBJKB_03087 0.67 +0.6
3,215,407 + OHPLBJKB_03087 0.67 +0.9
3,215,408 - OHPLBJKB_03087 0.67 -0.2
3,215,462 + OHPLBJKB_03087 0.72 -0.2
3,215,462 + OHPLBJKB_03087 0.72 +0.4
3,215,463 - OHPLBJKB_03087 0.72 -0.4
3,215,513 - OHPLBJKB_03087 0.77 +0.3
3,215,524 - OHPLBJKB_03087 0.78 +0.6
3,215,783 - +1.8
3,215,940 + OHPLBJKB_03088 0.56 -1.1
3,215,999 - OHPLBJKB_03088 0.75 -0.2
3,215,999 - OHPLBJKB_03088 0.75 +0.9
3,216,094 + +1.0
3,216,094 + +0.2
3,216,095 - +0.4
3,216,566 + OHPLBJKB_03089 0.55 -0.0
3,216,566 + OHPLBJKB_03089 0.55 -1.6
3,216,566 + OHPLBJKB_03089 0.55 -0.8
3,216,567 - OHPLBJKB_03089 0.55 -1.1
3,216,567 - OHPLBJKB_03089 0.55 +1.0
3,216,567 - OHPLBJKB_03089 0.55 -2.5
3,216,584 - OHPLBJKB_03089 0.57 +0.7
3,216,841 - OHPLBJKB_03089 0.85 -1.0
3,217,223 + OHPLBJKB_03090 0.15 -1.0
3,217,309 + OHPLBJKB_03090 0.21 +0.3
3,217,344 + OHPLBJKB_03090 0.23 +0.7
3,217,344 + OHPLBJKB_03090 0.23 -0.9
3,217,344 + OHPLBJKB_03090 0.23 -0.8
3,217,344 + OHPLBJKB_03090 0.23 -0.1
3,217,345 - OHPLBJKB_03090 0.23 +1.2
3,217,345 - OHPLBJKB_03090 0.23 -0.3
3,217,345 - OHPLBJKB_03090 0.23 -1.3
3,217,345 - OHPLBJKB_03090 0.23 -0.0
3,217,444 + OHPLBJKB_03090 0.30 -0.3
3,217,521 + OHPLBJKB_03090 0.35 +0.0
3,217,718 + OHPLBJKB_03090 0.48 +1.7

Or see this region's nucleotide sequence