Experiment: N4 MOI10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_02853 and OHPLBJKB_02854 overlap by 4 nucleotides OHPLBJKB_02854 and OHPLBJKB_02855 overlap by 1 nucleotides OHPLBJKB_02855 and OHPLBJKB_02856 are separated by 67 nucleotides OHPLBJKB_02856 and OHPLBJKB_02857 overlap by 37 nucleotides OHPLBJKB_02857 and OHPLBJKB_02858 are separated by 7 nucleotides OHPLBJKB_02858 and OHPLBJKB_02859 are separated by 32 nucleotides
OHPLBJKB_02853: OHPLBJKB_02853 - DNA adenine methylase, at 2,980,586 to 2,981,443
_02853
OHPLBJKB_02854: OHPLBJKB_02854 - putative protein YbiI, at 2,981,440 to 2,981,667
_02854
OHPLBJKB_02855: OHPLBJKB_02855 - hypothetical protein, at 2,981,667 to 2,981,900
_02855
OHPLBJKB_02856: OHPLBJKB_02856 - hypothetical protein, at 2,981,968 to 2,982,309
_02856
OHPLBJKB_02857: OHPLBJKB_02857 - hypothetical protein, at 2,982,273 to 2,982,473
_02857
OHPLBJKB_02858: OHPLBJKB_02858 - hypothetical protein, at 2,982,481 to 2,982,990
_02858
OHPLBJKB_02859: OHPLBJKB_02859 - hypothetical protein, at 2,983,023 to 2,983,244
_02859
Position (kb)
2981
2982
2983 Strain fitness (log2 ratio)
-2
-1
0
1 at 2980.969 kb on + strand, within OHPLBJKB_02853 at 2981.038 kb on - strand, within OHPLBJKB_02853 at 2981.038 kb on - strand, within OHPLBJKB_02853 at 2981.162 kb on - strand, within OHPLBJKB_02853 at 2981.443 kb on + strand at 2981.444 kb on - strand at 2981.459 kb on + strand at 2981.496 kb on + strand, within OHPLBJKB_02854 at 2981.496 kb on + strand, within OHPLBJKB_02854 at 2981.497 kb on - strand, within OHPLBJKB_02854 at 2981.497 kb on - strand, within OHPLBJKB_02854 at 2981.644 kb on + strand, within OHPLBJKB_02854 at 2981.644 kb on + strand, within OHPLBJKB_02854 at 2981.644 kb on + strand, within OHPLBJKB_02854 at 2981.669 kb on - strand at 2981.775 kb on - strand, within OHPLBJKB_02855 at 2981.892 kb on - strand at 2981.892 kb on - strand at 2981.894 kb on + strand at 2981.895 kb on - strand at 2981.919 kb on + strand at 2981.962 kb on - strand at 2982.247 kb on - strand, within OHPLBJKB_02856 at 2982.247 kb on - strand, within OHPLBJKB_02856 at 2982.247 kb on - strand, within OHPLBJKB_02856 at 2982.333 kb on + strand, within OHPLBJKB_02857 at 2982.334 kb on - strand, within OHPLBJKB_02857 at 2982.436 kb on - strand, within OHPLBJKB_02857 at 2982.473 kb on + strand at 2982.473 kb on + strand at 2982.473 kb on + strand at 2982.606 kb on + strand, within OHPLBJKB_02858 at 2982.607 kb on - strand, within OHPLBJKB_02858 at 2982.607 kb on - strand, within OHPLBJKB_02858 at 2982.646 kb on + strand, within OHPLBJKB_02858 at 2982.707 kb on + strand, within OHPLBJKB_02858 at 2982.707 kb on + strand, within OHPLBJKB_02858 at 2982.708 kb on - strand, within OHPLBJKB_02858 at 2982.708 kb on - strand, within OHPLBJKB_02858 at 2982.708 kb on - strand, within OHPLBJKB_02858 at 2982.784 kb on + strand, within OHPLBJKB_02858 at 2982.785 kb on - strand, within OHPLBJKB_02858 at 2982.785 kb on - strand, within OHPLBJKB_02858 at 2982.841 kb on + strand, within OHPLBJKB_02858 at 2983.074 kb on + strand, within OHPLBJKB_02859 at 2983.074 kb on + strand, within OHPLBJKB_02859 at 2983.074 kb on + strand, within OHPLBJKB_02859 at 2983.075 kb on - strand, within OHPLBJKB_02859 at 2983.190 kb on - strand, within OHPLBJKB_02859 at 2983.199 kb on + strand, within OHPLBJKB_02859 at 2983.200 kb on - strand, within OHPLBJKB_02859 at 2983.236 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N4 MOI10 remove 2,980,969 + OHPLBJKB_02853 0.45 +0.7 2,981,038 - OHPLBJKB_02853 0.53 +0.0 2,981,038 - OHPLBJKB_02853 0.53 +0.6 2,981,162 - OHPLBJKB_02853 0.67 -2.2 2,981,443 + -0.0 2,981,444 - -0.2 2,981,459 + -0.3 2,981,496 + OHPLBJKB_02854 0.25 +0.1 2,981,496 + OHPLBJKB_02854 0.25 -0.3 2,981,497 - OHPLBJKB_02854 0.25 -0.3 2,981,497 - OHPLBJKB_02854 0.25 -0.8 2,981,644 + OHPLBJKB_02854 0.89 -1.0 2,981,644 + OHPLBJKB_02854 0.89 -0.6 2,981,644 + OHPLBJKB_02854 0.89 +0.5 2,981,669 - +0.3 2,981,775 - OHPLBJKB_02855 0.46 -0.8 2,981,892 - -0.4 2,981,892 - -2.3 2,981,894 + -0.2 2,981,895 - +1.1 2,981,919 + -0.7 2,981,962 - -0.3 2,982,247 - OHPLBJKB_02856 0.82 -2.1 2,982,247 - OHPLBJKB_02856 0.82 -0.3 2,982,247 - OHPLBJKB_02856 0.82 -0.7 2,982,333 + OHPLBJKB_02857 0.30 -0.1 2,982,334 - OHPLBJKB_02857 0.30 -0.4 2,982,436 - OHPLBJKB_02857 0.81 -1.1 2,982,473 + +0.1 2,982,473 + -0.8 2,982,473 + +0.1 2,982,606 + OHPLBJKB_02858 0.25 -0.3 2,982,607 - OHPLBJKB_02858 0.25 -0.7 2,982,607 - OHPLBJKB_02858 0.25 +0.2 2,982,646 + OHPLBJKB_02858 0.32 +0.7 2,982,707 + OHPLBJKB_02858 0.44 +0.4 2,982,707 + OHPLBJKB_02858 0.44 -1.3 2,982,708 - OHPLBJKB_02858 0.45 -1.1 2,982,708 - OHPLBJKB_02858 0.45 -0.4 2,982,708 - OHPLBJKB_02858 0.45 -0.3 2,982,784 + OHPLBJKB_02858 0.59 -0.5 2,982,785 - OHPLBJKB_02858 0.60 -0.7 2,982,785 - OHPLBJKB_02858 0.60 -0.8 2,982,841 + OHPLBJKB_02858 0.71 +0.7 2,983,074 + OHPLBJKB_02859 0.23 -0.0 2,983,074 + OHPLBJKB_02859 0.23 +0.2 2,983,074 + OHPLBJKB_02859 0.23 -2.7 2,983,075 - OHPLBJKB_02859 0.23 -2.3 2,983,190 - OHPLBJKB_02859 0.75 -0.8 2,983,199 + OHPLBJKB_02859 0.79 +0.4 2,983,200 - OHPLBJKB_02859 0.80 -0.4 2,983,236 - -0.2
Or see this region's nucleotide sequence