Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_02856

Experiment: N4 MOI10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_02853 and OHPLBJKB_02854 overlap by 4 nucleotidesOHPLBJKB_02854 and OHPLBJKB_02855 overlap by 1 nucleotidesOHPLBJKB_02855 and OHPLBJKB_02856 are separated by 67 nucleotidesOHPLBJKB_02856 and OHPLBJKB_02857 overlap by 37 nucleotidesOHPLBJKB_02857 and OHPLBJKB_02858 are separated by 7 nucleotidesOHPLBJKB_02858 and OHPLBJKB_02859 are separated by 32 nucleotides OHPLBJKB_02853: OHPLBJKB_02853 - DNA adenine methylase, at 2,980,586 to 2,981,443 _02853 OHPLBJKB_02854: OHPLBJKB_02854 - putative protein YbiI, at 2,981,440 to 2,981,667 _02854 OHPLBJKB_02855: OHPLBJKB_02855 - hypothetical protein, at 2,981,667 to 2,981,900 _02855 OHPLBJKB_02856: OHPLBJKB_02856 - hypothetical protein, at 2,981,968 to 2,982,309 _02856 OHPLBJKB_02857: OHPLBJKB_02857 - hypothetical protein, at 2,982,273 to 2,982,473 _02857 OHPLBJKB_02858: OHPLBJKB_02858 - hypothetical protein, at 2,982,481 to 2,982,990 _02858 OHPLBJKB_02859: OHPLBJKB_02859 - hypothetical protein, at 2,983,023 to 2,983,244 _02859 Position (kb) 2981 2982 2983Strain fitness (log2 ratio) -2 -1 0 1at 2980.969 kb on + strand, within OHPLBJKB_02853at 2981.038 kb on - strand, within OHPLBJKB_02853at 2981.038 kb on - strand, within OHPLBJKB_02853at 2981.162 kb on - strand, within OHPLBJKB_02853at 2981.443 kb on + strandat 2981.444 kb on - strandat 2981.459 kb on + strandat 2981.496 kb on + strand, within OHPLBJKB_02854at 2981.496 kb on + strand, within OHPLBJKB_02854at 2981.497 kb on - strand, within OHPLBJKB_02854at 2981.497 kb on - strand, within OHPLBJKB_02854at 2981.644 kb on + strand, within OHPLBJKB_02854at 2981.644 kb on + strand, within OHPLBJKB_02854at 2981.644 kb on + strand, within OHPLBJKB_02854at 2981.669 kb on - strandat 2981.775 kb on - strand, within OHPLBJKB_02855at 2981.892 kb on - strandat 2981.892 kb on - strandat 2981.894 kb on + strandat 2981.895 kb on - strandat 2981.919 kb on + strandat 2981.962 kb on - strandat 2982.247 kb on - strand, within OHPLBJKB_02856at 2982.247 kb on - strand, within OHPLBJKB_02856at 2982.247 kb on - strand, within OHPLBJKB_02856at 2982.333 kb on + strand, within OHPLBJKB_02857at 2982.334 kb on - strand, within OHPLBJKB_02857at 2982.436 kb on - strand, within OHPLBJKB_02857at 2982.473 kb on + strandat 2982.473 kb on + strandat 2982.473 kb on + strandat 2982.606 kb on + strand, within OHPLBJKB_02858at 2982.607 kb on - strand, within OHPLBJKB_02858at 2982.607 kb on - strand, within OHPLBJKB_02858at 2982.646 kb on + strand, within OHPLBJKB_02858at 2982.707 kb on + strand, within OHPLBJKB_02858at 2982.707 kb on + strand, within OHPLBJKB_02858at 2982.708 kb on - strand, within OHPLBJKB_02858at 2982.708 kb on - strand, within OHPLBJKB_02858at 2982.708 kb on - strand, within OHPLBJKB_02858at 2982.784 kb on + strand, within OHPLBJKB_02858at 2982.785 kb on - strand, within OHPLBJKB_02858at 2982.785 kb on - strand, within OHPLBJKB_02858at 2982.841 kb on + strand, within OHPLBJKB_02858at 2983.074 kb on + strand, within OHPLBJKB_02859at 2983.074 kb on + strand, within OHPLBJKB_02859at 2983.074 kb on + strand, within OHPLBJKB_02859at 2983.075 kb on - strand, within OHPLBJKB_02859at 2983.190 kb on - strand, within OHPLBJKB_02859at 2983.199 kb on + strand, within OHPLBJKB_02859at 2983.200 kb on - strand, within OHPLBJKB_02859at 2983.236 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4 MOI10
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2,980,969 + OHPLBJKB_02853 0.45 +0.7
2,981,038 - OHPLBJKB_02853 0.53 +0.0
2,981,038 - OHPLBJKB_02853 0.53 +0.6
2,981,162 - OHPLBJKB_02853 0.67 -2.2
2,981,443 + -0.0
2,981,444 - -0.2
2,981,459 + -0.3
2,981,496 + OHPLBJKB_02854 0.25 +0.1
2,981,496 + OHPLBJKB_02854 0.25 -0.3
2,981,497 - OHPLBJKB_02854 0.25 -0.3
2,981,497 - OHPLBJKB_02854 0.25 -0.8
2,981,644 + OHPLBJKB_02854 0.89 -1.0
2,981,644 + OHPLBJKB_02854 0.89 -0.6
2,981,644 + OHPLBJKB_02854 0.89 +0.5
2,981,669 - +0.3
2,981,775 - OHPLBJKB_02855 0.46 -0.8
2,981,892 - -0.4
2,981,892 - -2.3
2,981,894 + -0.2
2,981,895 - +1.1
2,981,919 + -0.7
2,981,962 - -0.3
2,982,247 - OHPLBJKB_02856 0.82 -2.1
2,982,247 - OHPLBJKB_02856 0.82 -0.3
2,982,247 - OHPLBJKB_02856 0.82 -0.7
2,982,333 + OHPLBJKB_02857 0.30 -0.1
2,982,334 - OHPLBJKB_02857 0.30 -0.4
2,982,436 - OHPLBJKB_02857 0.81 -1.1
2,982,473 + +0.1
2,982,473 + -0.8
2,982,473 + +0.1
2,982,606 + OHPLBJKB_02858 0.25 -0.3
2,982,607 - OHPLBJKB_02858 0.25 -0.7
2,982,607 - OHPLBJKB_02858 0.25 +0.2
2,982,646 + OHPLBJKB_02858 0.32 +0.7
2,982,707 + OHPLBJKB_02858 0.44 +0.4
2,982,707 + OHPLBJKB_02858 0.44 -1.3
2,982,708 - OHPLBJKB_02858 0.45 -1.1
2,982,708 - OHPLBJKB_02858 0.45 -0.4
2,982,708 - OHPLBJKB_02858 0.45 -0.3
2,982,784 + OHPLBJKB_02858 0.59 -0.5
2,982,785 - OHPLBJKB_02858 0.60 -0.7
2,982,785 - OHPLBJKB_02858 0.60 -0.8
2,982,841 + OHPLBJKB_02858 0.71 +0.7
2,983,074 + OHPLBJKB_02859 0.23 -0.0
2,983,074 + OHPLBJKB_02859 0.23 +0.2
2,983,074 + OHPLBJKB_02859 0.23 -2.7
2,983,075 - OHPLBJKB_02859 0.23 -2.3
2,983,190 - OHPLBJKB_02859 0.75 -0.8
2,983,199 + OHPLBJKB_02859 0.79 +0.4
2,983,200 - OHPLBJKB_02859 0.80 -0.4
2,983,236 - -0.2

Or see this region's nucleotide sequence