Strain Fitness in Escherichia coli ECRC102 around NIAGMN_13455

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaL and rfaC overlap by 4 nucleotidesrfaC and rfaF are separated by 3 nucleotidesrfaF and rfaD are separated by 9 nucleotides NIAGMN_13445: rfaL - O-antigen ligase RfaL, at 2,656,723 to 2,657,931 rfaL NIAGMN_13450: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 2,657,928 to 2,658,920 rfaC NIAGMN_13455: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 2,658,924 to 2,659,970 rfaF NIAGMN_13460: rfaD - ADP-glyceromanno-heptose 6-epimerase, at 2,659,980 to 2,660,912 rfaD Position (kb) 2658 2659 2660Strain fitness (log2 ratio) -1 0 1at 2658.940 kb on - strandat 2658.940 kb on - strandat 2658.945 kb on + strandat 2658.967 kb on - strandat 2658.981 kb on - strandat 2659.466 kb on - strand, within rfaFat 2659.504 kb on - strand, within rfaFat 2659.504 kb on - strand, within rfaFat 2659.612 kb on - strand, within rfaFat 2659.631 kb on - strand, within rfaFat 2659.687 kb on - strand, within rfaFat 2659.840 kb on - strand, within rfaFat 2659.840 kb on - strand, within rfaFat 2659.982 kb on - strandat 2659.982 kb on - strandat 2660.931 kb on - strandat 2660.967 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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2,658,940 - +0.5
2,658,940 - +1.7
2,658,945 + +0.2
2,658,967 - -0.1
2,658,981 - +1.7
2,659,466 - rfaF NIAGMN_13455 0.52 -0.6
2,659,504 - rfaF NIAGMN_13455 0.55 -0.3
2,659,504 - rfaF NIAGMN_13455 0.55 +1.7
2,659,612 - rfaF NIAGMN_13455 0.66 +1.7
2,659,631 - rfaF NIAGMN_13455 0.68 +1.7
2,659,687 - rfaF NIAGMN_13455 0.73 -0.6
2,659,840 - rfaF NIAGMN_13455 0.87 -0.6
2,659,840 - rfaF NIAGMN_13455 0.87 +0.2
2,659,982 - -0.7
2,659,982 - +0.5
2,660,931 - -0.3
2,660,967 - -1.5

Or see this region's nucleotide sequence