Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS13600 and gshB are separated by 109 nucleotides gshB and pilG are separated by 314 nucleotides pilG and pilH are separated by 42 nucleotides
COO64_RS13600: COO64_RS13600 - energy transducer TonB, at 271,400 to 272,305
_RS13600
COO64_RS13605: gshB - glutathione synthase, at 272,415 to 273,368
gshB
COO64_RS13610: pilG - twitching motility response regulator PilG, at 273,683 to 274,093
pilG
COO64_RS13615: pilH - twitching motility response regulator PilH, at 274,136 to 274,501
pilH
Position (kb) 272
273
274 Strain fitness (log2 ratio) -2
-1
0
1
2 at 271.441 kb on + strand at 271.442 kb on - strand at 271.511 kb on + strand, within COO64_RS13600 at 271.511 kb on + strand, within COO64_RS13600 at 271.511 kb on + strand, within COO64_RS13600 at 271.511 kb on - strand, within COO64_RS13600 at 271.512 kb on - strand, within COO64_RS13600 at 271.512 kb on - strand, within COO64_RS13600 at 271.512 kb on - strand, within COO64_RS13600 at 271.512 kb on - strand, within COO64_RS13600 at 271.575 kb on + strand, within COO64_RS13600 at 271.575 kb on + strand, within COO64_RS13600 at 271.575 kb on + strand, within COO64_RS13600 at 271.576 kb on - strand, within COO64_RS13600 at 271.576 kb on - strand, within COO64_RS13600 at 271.576 kb on - strand, within COO64_RS13600 at 271.623 kb on + strand, within COO64_RS13600 at 271.653 kb on + strand, within COO64_RS13600 at 271.654 kb on - strand, within COO64_RS13600 at 271.701 kb on + strand, within COO64_RS13600 at 271.701 kb on + strand, within COO64_RS13600 at 271.701 kb on + strand, within COO64_RS13600 at 271.702 kb on - strand, within COO64_RS13600 at 271.702 kb on - strand, within COO64_RS13600 at 271.767 kb on + strand, within COO64_RS13600 at 271.767 kb on + strand, within COO64_RS13600 at 271.768 kb on - strand, within COO64_RS13600 at 271.768 kb on - strand, within COO64_RS13600 at 271.768 kb on - strand, within COO64_RS13600 at 271.768 kb on - strand, within COO64_RS13600 at 271.994 kb on - strand, within COO64_RS13600 at 271.994 kb on - strand, within COO64_RS13600 at 272.091 kb on + strand, within COO64_RS13600 at 272.092 kb on - strand, within COO64_RS13600 at 272.092 kb on - strand, within COO64_RS13600 at 272.092 kb on - strand, within COO64_RS13600 at 272.233 kb on + strand at 272.233 kb on + strand at 272.233 kb on + strand at 272.233 kb on + strand at 272.234 kb on - strand at 272.259 kb on - strand at 272.310 kb on + strand at 272.363 kb on + strand at 272.767 kb on + strand, within gshB at 272.767 kb on + strand, within gshB at 273.067 kb on + strand, within gshB at 273.067 kb on + strand, within gshB at 273.068 kb on - strand, within gshB at 273.068 kb on - strand, within gshB at 273.068 kb on - strand, within gshB at 273.068 kb on - strand, within gshB at 273.076 kb on + strand, within gshB at 273.235 kb on + strand, within gshB at 273.235 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.256 kb on + strand, within gshB at 273.257 kb on - strand, within gshB at 273.257 kb on - strand, within gshB at 273.257 kb on - strand, within gshB at 273.317 kb on + strand at 273.320 kb on - strand at 273.373 kb on + strand at 273.374 kb on - strand at 273.374 kb on - strand at 273.374 kb on - strand at 273.529 kb on - strand at 273.584 kb on + strand at 273.584 kb on + strand at 273.584 kb on + strand at 273.584 kb on + strand at 273.633 kb on + strand at 273.643 kb on + strand at 273.643 kb on + strand at 273.644 kb on - strand at 273.644 kb on - strand at 273.644 kb on - strand at 273.644 kb on - strand at 273.644 kb on - strand at 273.658 kb on - strand at 273.673 kb on + strand at 273.673 kb on + strand at 273.674 kb on - strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.682 kb on + strand at 273.683 kb on - strand at 273.756 kb on + strand, within pilG at 273.756 kb on + strand, within pilG at 273.756 kb on + strand, within pilG at 273.756 kb on + strand, within pilG at 273.756 kb on + strand, within pilG at 273.757 kb on - strand, within pilG at 273.830 kb on + strand, within pilG at 273.830 kb on + strand, within pilG at 273.831 kb on - strand, within pilG at 274.115 kb on + strand at 274.115 kb on + strand at 274.116 kb on - strand at 274.178 kb on + strand, within pilH at 274.178 kb on + strand, within pilH at 274.178 kb on + strand, within pilH at 274.192 kb on + strand, within pilH at 274.192 kb on + strand, within pilH at 274.193 kb on - strand, within pilH
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 271,441 + -1.4 271,442 - +0.2 271,511 + COO64_RS13600 0.12 +0.6 271,511 + COO64_RS13600 0.12 +0.4 271,511 + COO64_RS13600 0.12 -0.6 271,511 - COO64_RS13600 0.12 +0.3 271,512 - COO64_RS13600 0.12 +1.0 271,512 - COO64_RS13600 0.12 +1.4 271,512 - COO64_RS13600 0.12 -0.0 271,512 - COO64_RS13600 0.12 -1.6 271,575 + COO64_RS13600 0.19 -0.4 271,575 + COO64_RS13600 0.19 -0.4 271,575 + COO64_RS13600 0.19 +0.3 271,576 - COO64_RS13600 0.19 -0.0 271,576 - COO64_RS13600 0.19 +1.0 271,576 - COO64_RS13600 0.19 -0.2 271,623 + COO64_RS13600 0.25 -1.4 271,653 + COO64_RS13600 0.28 +0.5 271,654 - COO64_RS13600 0.28 -0.6 271,701 + COO64_RS13600 0.33 -0.9 271,701 + COO64_RS13600 0.33 +0.1 271,701 + COO64_RS13600 0.33 -0.4 271,702 - COO64_RS13600 0.33 +0.8 271,702 - COO64_RS13600 0.33 +0.8 271,767 + COO64_RS13600 0.41 -0.0 271,767 + COO64_RS13600 0.41 -1.2 271,768 - COO64_RS13600 0.41 +0.4 271,768 - COO64_RS13600 0.41 +1.8 271,768 - COO64_RS13600 0.41 +0.6 271,768 - COO64_RS13600 0.41 +0.4 271,994 - COO64_RS13600 0.66 +0.1 271,994 - COO64_RS13600 0.66 -0.3 272,091 + COO64_RS13600 0.76 +0.3 272,092 - COO64_RS13600 0.76 +0.1 272,092 - COO64_RS13600 0.76 +1.4 272,092 - COO64_RS13600 0.76 +0.8 272,233 + -2.1 272,233 + +0.2 272,233 + +0.5 272,233 + +0.0 272,234 - -0.7 272,259 - -0.0 272,310 + -0.1 272,363 + +2.4 272,767 + gshB COO64_RS13605 0.37 -1.0 272,767 + gshB COO64_RS13605 0.37 -0.5 273,067 + gshB COO64_RS13605 0.68 -0.0 273,067 + gshB COO64_RS13605 0.68 +2.4 273,068 - gshB COO64_RS13605 0.68 +1.4 273,068 - gshB COO64_RS13605 0.68 -2.1 273,068 - gshB COO64_RS13605 0.68 -0.6 273,068 - gshB COO64_RS13605 0.68 -0.9 273,076 + gshB COO64_RS13605 0.69 +0.6 273,235 + gshB COO64_RS13605 0.86 -0.4 273,235 + gshB COO64_RS13605 0.86 +1.2 273,256 + gshB COO64_RS13605 0.88 -0.4 273,256 + gshB COO64_RS13605 0.88 -0.0 273,256 + gshB COO64_RS13605 0.88 -0.9 273,256 + gshB COO64_RS13605 0.88 +1.0 273,256 + gshB COO64_RS13605 0.88 -0.8 273,256 + gshB COO64_RS13605 0.88 -1.0 273,256 + gshB COO64_RS13605 0.88 +0.3 273,257 - gshB COO64_RS13605 0.88 +0.4 273,257 - gshB COO64_RS13605 0.88 +1.2 273,257 - gshB COO64_RS13605 0.88 +0.4 273,317 + +1.0 273,320 - -1.1 273,373 + +0.6 273,374 - +0.2 273,374 - +0.0 273,374 - -0.6 273,529 - -0.7 273,584 + +0.3 273,584 + +0.5 273,584 + +0.6 273,584 + -1.1 273,633 + -0.6 273,643 + -0.2 273,643 + +0.2 273,644 - +0.6 273,644 - -1.1 273,644 - -0.8 273,644 - +0.5 273,644 - +0.6 273,658 - -1.7 273,673 + +0.6 273,673 + -0.4 273,674 - -0.6 273,682 + +0.2 273,682 + +0.7 273,682 + -1.4 273,682 + -0.6 273,682 + -0.2 273,682 + +0.6 273,682 + -0.1 273,682 + -0.8 273,683 - -0.4 273,756 + pilG COO64_RS13610 0.18 +0.1 273,756 + pilG COO64_RS13610 0.18 -0.9 273,756 + pilG COO64_RS13610 0.18 +0.5 273,756 + pilG COO64_RS13610 0.18 -0.8 273,756 + pilG COO64_RS13610 0.18 -0.1 273,757 - pilG COO64_RS13610 0.18 +0.9 273,830 + pilG COO64_RS13610 0.36 +0.3 273,830 + pilG COO64_RS13610 0.36 +0.5 273,831 - pilG COO64_RS13610 0.36 -0.6 274,115 + -0.4 274,115 + +0.6 274,116 - +0.7 274,178 + pilH COO64_RS13615 0.11 +0.9 274,178 + pilH COO64_RS13615 0.11 +1.2 274,178 + pilH COO64_RS13615 0.11 -1.1 274,192 + pilH COO64_RS13615 0.15 +0.3 274,192 + pilH COO64_RS13615 0.15 -0.8 274,193 - pilH COO64_RS13615 0.16 -1.5
Or see this region's nucleotide sequence