Strain Fitness in Pseudomonas sp. SVBP6 around COO64_RS04930

Experiment: supernatant control, M9 with 0.25X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCOO64_RS26850 and COO64_RS04925 are separated by 73 nucleotidesCOO64_RS04925 and dsbC are separated by 174 nucleotidesdsbC and COO64_RS04935 are separated by 79 nucleotides COO64_RS26850: COO64_RS26850 - hypothetical protein, at 476,669 to 476,983 _RS26850 COO64_RS04925: COO64_RS04925 - hypothetical protein, at 477,057 to 477,515 _RS04925 COO64_RS04930: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 477,690 to 478,418 dsbC COO64_RS04935: COO64_RS04935 - homoserine dehydrogenase, at 478,498 to 479,802 _RS04935 Position (kb) 477 478 479Strain fitness (log2 ratio) -2 -1 0 1at 476.722 kb on + strand, within COO64_RS26850at 476.741 kb on + strand, within COO64_RS26850at 476.741 kb on + strand, within COO64_RS26850at 476.741 kb on + strand, within COO64_RS26850at 476.742 kb on - strand, within COO64_RS26850at 476.796 kb on - strand, within COO64_RS26850at 476.807 kb on + strand, within COO64_RS26850at 476.839 kb on + strand, within COO64_RS26850at 476.839 kb on + strand, within COO64_RS26850at 476.840 kb on - strand, within COO64_RS26850at 476.840 kb on - strand, within COO64_RS26850at 476.875 kb on + strand, within COO64_RS26850at 476.883 kb on - strand, within COO64_RS26850at 476.914 kb on - strand, within COO64_RS26850at 477.041 kb on - strandat 477.041 kb on - strandat 477.136 kb on + strand, within COO64_RS04925at 477.137 kb on - strand, within COO64_RS04925at 477.172 kb on + strand, within COO64_RS04925at 477.173 kb on - strand, within COO64_RS04925at 477.176 kb on + strand, within COO64_RS04925at 477.176 kb on + strand, within COO64_RS04925at 477.177 kb on - strand, within COO64_RS04925at 477.177 kb on - strand, within COO64_RS04925at 477.186 kb on - strand, within COO64_RS04925at 477.186 kb on - strand, within COO64_RS04925at 477.237 kb on + strand, within COO64_RS04925at 477.237 kb on + strand, within COO64_RS04925at 477.238 kb on - strand, within COO64_RS04925at 477.238 kb on - strand, within COO64_RS04925at 477.238 kb on - strand, within COO64_RS04925at 477.442 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.451 kb on + strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.452 kb on - strand, within COO64_RS04925at 477.470 kb on + strandat 477.470 kb on + strandat 477.471 kb on - strandat 477.471 kb on - strandat 477.471 kb on - strandat 477.471 kb on - strandat 477.480 kb on + strandat 477.480 kb on + strandat 477.481 kb on - strandat 477.481 kb on - strandat 477.513 kb on + strandat 477.514 kb on - strandat 477.514 kb on - strandat 477.541 kb on - strandat 477.541 kb on - strandat 477.541 kb on - strandat 477.595 kb on - strandat 477.737 kb on + strandat 477.738 kb on - strandat 477.738 kb on - strandat 477.738 kb on - strandat 477.849 kb on + strand, within dsbCat 477.849 kb on + strand, within dsbCat 477.849 kb on + strand, within dsbCat 477.849 kb on + strand, within dsbCat 477.850 kb on - strand, within dsbCat 477.850 kb on - strand, within dsbCat 477.850 kb on - strand, within dsbCat 477.850 kb on - strand, within dsbCat 477.850 kb on - strand, within dsbCat 477.882 kb on + strand, within dsbCat 477.882 kb on + strand, within dsbCat 477.883 kb on - strand, within dsbCat 477.883 kb on - strand, within dsbCat 477.883 kb on - strand, within dsbCat 478.026 kb on + strand, within dsbCat 478.026 kb on + strand, within dsbCat 478.026 kb on + strand, within dsbCat 478.067 kb on + strand, within dsbCat 478.067 kb on + strand, within dsbCat 478.111 kb on + strand, within dsbCat 478.111 kb on + strand, within dsbCat 478.146 kb on + strand, within dsbCat 478.146 kb on + strand, within dsbCat 478.416 kb on + strandat 478.416 kb on + strandat 478.416 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB
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476,722 + COO64_RS26850 0.17 -0.2
476,741 + COO64_RS26850 0.23 +0.3
476,741 + COO64_RS26850 0.23 -0.3
476,741 + COO64_RS26850 0.23 -0.3
476,742 - COO64_RS26850 0.23 +1.6
476,796 - COO64_RS26850 0.40 +0.2
476,807 + COO64_RS26850 0.44 +0.2
476,839 + COO64_RS26850 0.54 -0.4
476,839 + COO64_RS26850 0.54 -0.0
476,840 - COO64_RS26850 0.54 -0.0
476,840 - COO64_RS26850 0.54 -0.2
476,875 + COO64_RS26850 0.65 -0.5
476,883 - COO64_RS26850 0.68 -0.5
476,914 - COO64_RS26850 0.78 +1.1
477,041 - +0.6
477,041 - +0.2
477,136 + COO64_RS04925 0.17 +0.0
477,137 - COO64_RS04925 0.17 -0.8
477,172 + COO64_RS04925 0.25 -0.1
477,173 - COO64_RS04925 0.25 +0.4
477,176 + COO64_RS04925 0.26 -0.0
477,176 + COO64_RS04925 0.26 +0.2
477,177 - COO64_RS04925 0.26 -0.6
477,177 - COO64_RS04925 0.26 -0.6
477,186 - COO64_RS04925 0.28 -0.5
477,186 - COO64_RS04925 0.28 +0.5
477,237 + COO64_RS04925 0.39 -0.2
477,237 + COO64_RS04925 0.39 +0.7
477,238 - COO64_RS04925 0.39 +0.0
477,238 - COO64_RS04925 0.39 -0.6
477,238 - COO64_RS04925 0.39 +1.0
477,442 + COO64_RS04925 0.84 +0.4
477,451 + COO64_RS04925 0.86 +0.9
477,451 + COO64_RS04925 0.86 -1.1
477,451 + COO64_RS04925 0.86 +0.4
477,451 + COO64_RS04925 0.86 +0.3
477,451 + COO64_RS04925 0.86 +0.9
477,451 + COO64_RS04925 0.86 -0.1
477,451 + COO64_RS04925 0.86 +0.5
477,451 + COO64_RS04925 0.86 -0.0
477,452 - COO64_RS04925 0.86 -0.0
477,452 - COO64_RS04925 0.86 -1.7
477,452 - COO64_RS04925 0.86 -0.5
477,452 - COO64_RS04925 0.86 +0.2
477,452 - COO64_RS04925 0.86 -0.6
477,452 - COO64_RS04925 0.86 -0.1
477,452 - COO64_RS04925 0.86 +0.0
477,452 - COO64_RS04925 0.86 +1.0
477,452 - COO64_RS04925 0.86 -0.3
477,452 - COO64_RS04925 0.86 -0.9
477,470 + -0.6
477,470 + -0.7
477,471 - +0.5
477,471 - -0.9
477,471 - +0.5
477,471 - +0.2
477,480 + +0.9
477,480 + -0.5
477,481 - +0.2
477,481 - +0.3
477,513 + -0.9
477,514 - +1.3
477,514 - -0.0
477,541 - -0.9
477,541 - -1.5
477,541 - +0.0
477,595 - +0.1
477,737 + -1.3
477,738 - +0.6
477,738 - +1.0
477,738 - -1.8
477,849 + dsbC COO64_RS04930 0.22 -0.0
477,849 + dsbC COO64_RS04930 0.22 -0.2
477,849 + dsbC COO64_RS04930 0.22 -0.4
477,849 + dsbC COO64_RS04930 0.22 -0.6
477,850 - dsbC COO64_RS04930 0.22 -1.4
477,850 - dsbC COO64_RS04930 0.22 +0.6
477,850 - dsbC COO64_RS04930 0.22 -2.1
477,850 - dsbC COO64_RS04930 0.22 -1.7
477,850 - dsbC COO64_RS04930 0.22 +1.0
477,882 + dsbC COO64_RS04930 0.26 -0.7
477,882 + dsbC COO64_RS04930 0.26 -0.4
477,883 - dsbC COO64_RS04930 0.26 -1.5
477,883 - dsbC COO64_RS04930 0.26 +0.1
477,883 - dsbC COO64_RS04930 0.26 -0.8
478,026 + dsbC COO64_RS04930 0.46 -0.4
478,026 + dsbC COO64_RS04930 0.46 -0.8
478,026 + dsbC COO64_RS04930 0.46 -0.5
478,067 + dsbC COO64_RS04930 0.52 -0.6
478,067 + dsbC COO64_RS04930 0.52 -0.6
478,111 + dsbC COO64_RS04930 0.58 -0.6
478,111 + dsbC COO64_RS04930 0.58 -1.5
478,146 + dsbC COO64_RS04930 0.63 -0.2
478,146 + dsbC COO64_RS04930 0.63 -0.5
478,416 + +0.2
478,416 + -0.9
478,416 + -0.7

Or see this region's nucleotide sequence