Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3077 and GFF3078 are separated by 67 nucleotides GFF3078 and GFF3079 are separated by 56 nucleotides GFF3079 and GFF3080 are separated by 95 nucleotides GFF3080 and GFF3081 are separated by 11 nucleotides
GFF3077 - Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), at 153,807 to 155,426
GFF3077
GFF3078 - FIG00581584: hypothetical protein, at 155,494 to 156,303
GFF3078
GFF3079 - Transcriptional regulator, TetR family, at 156,360 to 156,932
GFF3079
GFF3080 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 157,028 to 157,489
GFF3080
GFF3081 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 157,501 to 159,765
GFF3081
Position (kb)
156
157 Strain fitness (log2 ratio)
-2
-1
0
1 at 155.389 kb on + strand at 155.390 kb on - strand at 155.390 kb on - strand at 155.390 kb on - strand at 155.402 kb on - strand at 155.402 kb on - strand at 155.454 kb on + strand at 155.454 kb on + strand at 155.454 kb on + strand at 155.454 kb on + strand at 155.455 kb on - strand at 155.455 kb on - strand at 155.471 kb on + strand at 155.471 kb on + strand at 155.471 kb on + strand at 155.471 kb on + strand at 155.471 kb on + strand at 155.472 kb on - strand at 155.548 kb on + strand at 155.549 kb on - strand at 155.549 kb on - strand at 155.549 kb on - strand at 155.549 kb on - strand at 155.549 kb on - strand at 155.549 kb on - strand at 155.549 kb on - strand at 155.755 kb on + strand, within GFF3078 at 155.756 kb on - strand, within GFF3078 at 155.756 kb on - strand, within GFF3078 at 155.756 kb on - strand, within GFF3078 at 155.848 kb on + strand, within GFF3078 at 155.848 kb on + strand, within GFF3078 at 155.848 kb on + strand, within GFF3078 at 155.848 kb on + strand, within GFF3078 at 155.849 kb on - strand, within GFF3078 at 156.172 kb on + strand, within GFF3078 at 156.172 kb on + strand, within GFF3078 at 156.172 kb on + strand, within GFF3078 at 156.172 kb on + strand, within GFF3078 at 156.173 kb on - strand, within GFF3078 at 156.280 kb on + strand at 156.280 kb on + strand at 156.281 kb on - strand at 156.281 kb on - strand at 156.333 kb on + strand at 156.485 kb on + strand, within GFF3079 at 156.485 kb on + strand, within GFF3079 at 156.486 kb on - strand, within GFF3079 at 156.486 kb on - strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.492 kb on + strand, within GFF3079 at 156.493 kb on - strand, within GFF3079 at 156.493 kb on - strand, within GFF3079 at 156.493 kb on - strand, within GFF3079 at 156.493 kb on - strand, within GFF3079 at 156.493 kb on - strand, within GFF3079 at 156.498 kb on + strand, within GFF3079 at 156.498 kb on + strand, within GFF3079 at 156.499 kb on - strand, within GFF3079 at 156.499 kb on - strand, within GFF3079 at 156.499 kb on - strand, within GFF3079 at 156.499 kb on - strand, within GFF3079 at 156.499 kb on - strand, within GFF3079 at 156.534 kb on + strand, within GFF3079 at 156.534 kb on + strand, within GFF3079 at 156.535 kb on - strand, within GFF3079 at 156.535 kb on - strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.682 kb on - strand, within GFF3079 at 156.682 kb on - strand, within GFF3079 at 156.684 kb on + strand, within GFF3079 at 156.684 kb on + strand, within GFF3079 at 156.684 kb on + strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.969 kb on - strand at 156.995 kb on + strand at 156.996 kb on - strand at 156.996 kb on - strand at 157.002 kb on - strand at 157.002 kb on - strand at 157.439 kb on + strand, within GFF3080 at 157.439 kb on + strand, within GFF3080 at 157.440 kb on - strand, within GFF3080 at 157.440 kb on - strand, within GFF3080 at 157.440 kb on - strand, within GFF3080 at 157.652 kb on - strand at 157.885 kb on + strand, within GFF3081 at 157.885 kb on + strand, within GFF3081 at 157.886 kb on - strand, within GFF3081 at 157.886 kb on - strand, within GFF3081
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 155,389 + +0.1 155,390 - -1.4 155,390 - +0.0 155,390 - +0.4 155,402 - +1.2 155,402 - -0.0 155,454 + -2.5 155,454 + -0.6 155,454 + -1.8 155,454 + -0.1 155,455 - -0.9 155,455 - -0.0 155,471 + +0.1 155,471 + -0.5 155,471 + -0.8 155,471 + +0.4 155,471 + +0.3 155,472 - +0.3 155,548 + -0.1 155,549 - +0.2 155,549 - +0.3 155,549 - -0.4 155,549 - +1.3 155,549 - +0.7 155,549 - -0.6 155,549 - +0.1 155,755 + GFF3078 0.32 +1.1 155,756 - GFF3078 0.32 -0.7 155,756 - GFF3078 0.32 +0.8 155,756 - GFF3078 0.32 -0.3 155,848 + GFF3078 0.44 +0.4 155,848 + GFF3078 0.44 +0.6 155,848 + GFF3078 0.44 -0.8 155,848 + GFF3078 0.44 +0.6 155,849 - GFF3078 0.44 -0.6 156,172 + GFF3078 0.84 +0.2 156,172 + GFF3078 0.84 +0.3 156,172 + GFF3078 0.84 +1.3 156,172 + GFF3078 0.84 +0.2 156,173 - GFF3078 0.84 +0.2 156,280 + +0.1 156,280 + -0.0 156,281 - -0.1 156,281 - -0.2 156,333 + -2.1 156,485 + GFF3079 0.22 +0.1 156,485 + GFF3079 0.22 -1.4 156,486 - GFF3079 0.22 +0.2 156,486 - GFF3079 0.22 -0.0 156,492 + GFF3079 0.23 -0.6 156,492 + GFF3079 0.23 +0.1 156,492 + GFF3079 0.23 -0.0 156,492 + GFF3079 0.23 -0.9 156,492 + GFF3079 0.23 -1.9 156,492 + GFF3079 0.23 -0.6 156,492 + GFF3079 0.23 +0.2 156,492 + GFF3079 0.23 +0.6 156,492 + GFF3079 0.23 +1.1 156,493 - GFF3079 0.23 -0.0 156,493 - GFF3079 0.23 +1.3 156,493 - GFF3079 0.23 +0.4 156,493 - GFF3079 0.23 +1.1 156,493 - GFF3079 0.23 +0.2 156,498 + GFF3079 0.24 +0.0 156,498 + GFF3079 0.24 +0.1 156,499 - GFF3079 0.24 -0.1 156,499 - GFF3079 0.24 +0.1 156,499 - GFF3079 0.24 +0.1 156,499 - GFF3079 0.24 -0.7 156,499 - GFF3079 0.24 +0.4 156,534 + GFF3079 0.30 -0.3 156,534 + GFF3079 0.30 -0.5 156,535 - GFF3079 0.31 +0.5 156,535 - GFF3079 0.31 -0.3 156,681 + GFF3079 0.56 -1.8 156,681 + GFF3079 0.56 -0.6 156,681 + GFF3079 0.56 -0.3 156,681 + GFF3079 0.56 -0.5 156,682 - GFF3079 0.56 +0.1 156,682 - GFF3079 0.56 -0.7 156,684 + GFF3079 0.57 -0.0 156,684 + GFF3079 0.57 +0.1 156,684 + GFF3079 0.57 -0.7 156,685 - GFF3079 0.57 -0.2 156,685 - GFF3079 0.57 +0.2 156,685 - GFF3079 0.57 -0.4 156,685 - GFF3079 0.57 +0.3 156,685 - GFF3079 0.57 -2.0 156,969 - -0.0 156,995 + +0.1 156,996 - -0.2 156,996 - +0.6 157,002 - -0.3 157,002 - +0.8 157,439 + GFF3080 0.89 +0.0 157,439 + GFF3080 0.89 -0.5 157,440 - GFF3080 0.89 +0.7 157,440 - GFF3080 0.89 -1.3 157,440 - GFF3080 0.89 -0.2 157,652 - -0.1 157,885 + GFF3081 0.17 +0.4 157,885 + GFF3081 0.17 -0.3 157,886 - GFF3081 0.17 -0.4 157,886 - GFF3081 0.17 +0.4
Or see this region's nucleotide sequence