Experiment: Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS20995 and COO64_RS21000 are separated by 10 nucleotides COO64_RS21000 and COO64_RS21005 are separated by 14 nucleotides
COO64_RS20995: COO64_RS20995 - 5-guanidino-2-oxopentanoate decarboxylase, at 105,645 to 107,252
_RS20995
COO64_RS21000: COO64_RS21000 - acetate--CoA ligase family protein, at 107,263 to 109,353
_RS21000
COO64_RS21005: COO64_RS21005 - acyl-CoA dehydrogenase family protein, at 109,368 to 110,528
_RS21005
Position (kb)
107
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 106.327 kb on + strand, within COO64_RS20995 at 106.327 kb on + strand, within COO64_RS20995 at 106.327 kb on + strand, within COO64_RS20995 at 106.327 kb on + strand, within COO64_RS20995 at 106.766 kb on + strand, within COO64_RS20995 at 106.766 kb on + strand, within COO64_RS20995 at 106.766 kb on + strand, within COO64_RS20995 at 106.766 kb on + strand, within COO64_RS20995 at 106.767 kb on - strand, within COO64_RS20995 at 106.767 kb on - strand, within COO64_RS20995 at 107.146 kb on + strand at 107.147 kb on - strand at 107.147 kb on - strand at 107.147 kb on - strand at 107.197 kb on + strand at 107.197 kb on + strand at 107.198 kb on - strand at 107.198 kb on - strand at 107.264 kb on + strand at 107.264 kb on + strand at 107.264 kb on + strand at 107.264 kb on + strand at 107.264 kb on + strand at 107.264 kb on + strand at 107.264 kb on + strand at 107.266 kb on + strand at 107.266 kb on + strand at 107.266 kb on + strand at 107.266 kb on + strand at 107.267 kb on - strand at 107.267 kb on - strand at 107.267 kb on - strand at 107.372 kb on + strand at 107.660 kb on - strand, within COO64_RS21000 at 107.672 kb on + strand, within COO64_RS21000 at 108.162 kb on - strand, within COO64_RS21000 at 108.162 kb on - strand, within COO64_RS21000 at 108.338 kb on + strand, within COO64_RS21000 at 108.339 kb on - strand, within COO64_RS21000 at 108.339 kb on - strand, within COO64_RS21000 at 108.416 kb on + strand, within COO64_RS21000 at 108.417 kb on - strand, within COO64_RS21000 at 108.417 kb on - strand, within COO64_RS21000 at 108.824 kb on + strand, within COO64_RS21000 at 108.824 kb on + strand, within COO64_RS21000 at 108.824 kb on + strand, within COO64_RS21000 at 108.825 kb on - strand, within COO64_RS21000 at 108.825 kb on - strand, within COO64_RS21000 at 108.825 kb on - strand, within COO64_RS21000 at 108.863 kb on + strand, within COO64_RS21000 at 108.864 kb on - strand, within COO64_RS21000 at 108.957 kb on - strand, within COO64_RS21000 at 108.971 kb on + strand, within COO64_RS21000 at 108.971 kb on + strand, within COO64_RS21000 at 108.972 kb on - strand, within COO64_RS21000 at 109.071 kb on - strand, within COO64_RS21000 at 109.147 kb on - strand at 109.151 kb on - strand at 109.508 kb on - strand, within COO64_RS21005 at 109.687 kb on + strand, within COO64_RS21005 at 109.687 kb on + strand, within COO64_RS21005 at 109.687 kb on + strand, within COO64_RS21005 at 109.687 kb on + strand, within COO64_RS21005 at 109.688 kb on - strand, within COO64_RS21005 at 109.900 kb on + strand, within COO64_RS21005 at 110.008 kb on + strand, within COO64_RS21005 at 110.008 kb on + strand, within COO64_RS21005 at 110.008 kb on + strand, within COO64_RS21005 at 110.321 kb on - strand, within COO64_RS21005 at 110.321 kb on - strand, within COO64_RS21005
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9 remove 106,327 + COO64_RS20995 0.42 +0.4 106,327 + COO64_RS20995 0.42 +1.1 106,327 + COO64_RS20995 0.42 +0.4 106,327 + COO64_RS20995 0.42 +0.1 106,766 + COO64_RS20995 0.70 +0.6 106,766 + COO64_RS20995 0.70 -0.8 106,766 + COO64_RS20995 0.70 +0.2 106,766 + COO64_RS20995 0.70 -0.7 106,767 - COO64_RS20995 0.70 -0.9 106,767 - COO64_RS20995 0.70 +1.3 107,146 + -0.8 107,147 - -0.5 107,147 - +1.5 107,147 - +0.6 107,197 + +0.6 107,197 + -0.3 107,198 - -0.2 107,198 - -0.0 107,264 + -0.6 107,264 + +0.5 107,264 + -0.8 107,264 + -0.3 107,264 + -1.3 107,264 + -0.2 107,264 + +1.0 107,266 + -1.1 107,266 + -0.4 107,266 + -0.3 107,266 + +0.1 107,267 - -0.3 107,267 - -0.3 107,267 - +0.3 107,372 + -0.2 107,660 - COO64_RS21000 0.19 -0.2 107,672 + COO64_RS21000 0.20 +0.1 108,162 - COO64_RS21000 0.43 -0.9 108,162 - COO64_RS21000 0.43 -1.8 108,338 + COO64_RS21000 0.51 +0.4 108,339 - COO64_RS21000 0.51 -0.7 108,339 - COO64_RS21000 0.51 -0.4 108,416 + COO64_RS21000 0.55 -0.1 108,417 - COO64_RS21000 0.55 -0.3 108,417 - COO64_RS21000 0.55 +1.8 108,824 + COO64_RS21000 0.75 +0.6 108,824 + COO64_RS21000 0.75 -1.5 108,824 + COO64_RS21000 0.75 -0.6 108,825 - COO64_RS21000 0.75 +0.5 108,825 - COO64_RS21000 0.75 -1.2 108,825 - COO64_RS21000 0.75 +0.4 108,863 + COO64_RS21000 0.77 +1.2 108,864 - COO64_RS21000 0.77 -2.6 108,957 - COO64_RS21000 0.81 -0.7 108,971 + COO64_RS21000 0.82 +0.0 108,971 + COO64_RS21000 0.82 +0.1 108,972 - COO64_RS21000 0.82 +0.1 109,071 - COO64_RS21000 0.86 -2.1 109,147 - -1.6 109,151 - -0.3 109,508 - COO64_RS21005 0.12 -0.3 109,687 + COO64_RS21005 0.27 -0.1 109,687 + COO64_RS21005 0.27 -0.2 109,687 + COO64_RS21005 0.27 -0.2 109,687 + COO64_RS21005 0.27 -0.2 109,688 - COO64_RS21005 0.28 -0.8 109,900 + COO64_RS21005 0.46 +0.9 110,008 + COO64_RS21005 0.55 +1.0 110,008 + COO64_RS21005 0.55 +2.0 110,008 + COO64_RS21005 0.55 -0.4 110,321 - COO64_RS21005 0.82 +0.2 110,321 - COO64_RS21005 0.82 +0.5
Or see this region's nucleotide sequence